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Species: E. coli
Genetic Insert: Cas1
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCDF1b; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:28729350
Proper citation: RRID:Addgene_113238 Copy
Species: E. coli
Genetic Insert: Cas1
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCDF1b; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:28729350
Proper citation: RRID:Addgene_113185 Copy
Species: E. coli
Genetic Insert: Cas1
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCDF1b; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:28729350
Proper citation: RRID:Addgene_113189 Copy
Species: E. coli
Genetic Insert: Cas1
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCDF1b; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:28729350
Proper citation: RRID:Addgene_113178 Copy
Species: E. coli
Genetic Insert: Cas1
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCDF1b; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:28729350
Proper citation: RRID:Addgene_113177 Copy
Species: E. coli
Genetic Insert: Cas1
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCDF1b; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:28729350
Proper citation: RRID:Addgene_113175 Copy
Species: E. coli
Genetic Insert: Cas1
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCDF1b; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:28729350
Proper citation: RRID:Addgene_113179 Copy
Species: E. coli
Genetic Insert: Cas1
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCDF1b; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:28729350
Proper citation: RRID:Addgene_113180 Copy
Species: E. coli
Genetic Insert: tdNfsB(F124W)-mCherry
Vector Backbone Description: Vector Backbone:AAV2; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30111097
Proper citation: RRID:Addgene_113760 Copy
Species: E. coli
Genetic Insert: tdNfsB(F124W)-mCherry
Vector Backbone Description: Vector Backbone:AAV2; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30111097
Proper citation: RRID:Addgene_113761 Copy
Species: E. coli
Genetic Insert: tdNfsB(F124W)-mCherry
Vector Backbone Description: Backbone Marker:Scott Sternson; Vector Backbone:AAV2; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30111097
Comments: Sternson Lab recommendations for propagation of AAV-FLEX plasmids: "These vectors are prone to recombination. This is a well known issue with these AAV vectors and is due to the inverted terminal repeats (ITRs) required for AAV production. To minimize recombination, we propagate these plasmids in NEB Stable cells. Also, to minimize recombination, cells should be cultured at 30 C.
Note that these cultures will grow slowly (20 h for minipreps). Better yields and culture times are obtained with 2xYT as the media. This is strongly recommended.
Because recombination may still happen occasionally, we do a panel of restriction digestions to assess whether the ITRs are in tact. Separate digestions with PvuII, Sma1, and SnaB1 should be performed."
Proper citation: RRID:Addgene_113763 Copy
Species: E. coli
Genetic Insert: gRNA
Vector Backbone Description: Vector Backbone:psgRNA; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29765036
Proper citation: RRID:Addgene_114006 Copy
Species: E. coli
Genetic Insert: colicin E1 immunity protein
Vector Backbone Description: Backbone Marker:Novagen/EMD Millipore; Backbone Size:5227; Vector Backbone:pET52-b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27795317
Comments: This protein was never expressed independently. It was cloned in order to subsequently clone a colicin gene, which would be lethal in the absence of the immunity protein. The cloned fragment was from base pair 500 to base pair 48 of ColE1, which encompasses the E1 immunity protein promoter and the gene.
Proper citation: RRID:Addgene_103064 Copy
Species: E. coli
Genetic Insert: MotA
Vector Backbone Description: Vector Backbone:pBAD33; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28974721
Proper citation: RRID:Addgene_158437 Copy
Species: E. coli
Genetic Insert: MotA
Vector Backbone Description: Vector Backbone:pBAD33; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28974721
Proper citation: RRID:Addgene_158436 Copy
Species: E. coli
Genetic Insert: MotA
Vector Backbone Description: Vector Backbone:pBAD33; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28974721
Proper citation: RRID:Addgene_158434 Copy
Species: E. coli
Genetic Insert: LexA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5600; Vector Backbone:pcDNA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15203006
Proper citation: RRID:Addgene_14593 Copy
Species: E. coli
Genetic Insert: LexA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5600; Vector Backbone:pcDNA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15203006
Proper citation: RRID:Addgene_14591 Copy
Species: E. coli
Genetic Insert: MBP
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16581772
Comments: Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_146120 Copy
Species: E. coli
Genetic Insert: gRNA targeting mqo
Vector Backbone Description: Vector Backbone:pKD46; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33346713
Proper citation: RRID:Addgene_154921 Copy
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