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Species: Synthetic
Genetic Insert: Caprola_06-mEGFP
Vector Backbone Description: Backbone Size:7700; Vector Backbone:pLKO.1; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38386755
Proper citation: RRID:Addgene_194693 Copy
Species: Arabidopsis thaliana
Genetic Insert: HR1b
Vector Backbone Description: Backbone Marker:Diego Orzaez Lab; Backbone Size:6369; Vector Backbone:pDGB3_alpha2; Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:38446628
Comments: Rojas-Pierce Lab plasmid pMRP1201, Design 98
Proper citation: RRID:Addgene_211847 Copy
Genetic Insert: shCdh2.1
Vector Backbone Description: Vector Backbone:pLKO.1; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37707499
Proper citation: RRID:Addgene_209099 Copy
Genetic Insert: Plastid transit peptide fused sfGFP
Vector Backbone Description: Vector Backbone:pGWB508; Vector Types:Bacterial Expression, Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:36705704
Proper citation: RRID:Addgene_213863 Copy
Species: Arabidopsis thaliana
Genetic Insert: GRANULE-BOUND STARCH SYNTHASE I (GBSSI)
Vector Backbone Description: Vector Backbone:pGWB605; Vector Types:Bacterial Expression, Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37792703
Proper citation: RRID:Addgene_213867 Copy
Species: Salmonella typhimurium
Genetic Insert: LRR-NEL
Vector Backbone Description: Backbone Marker:Diego Orzaez Lab; Backbone Size:6367; Vector Backbone:pDGB3_alpha1; Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:38446628
Comments: Rojas-Pierce Lab plasmid pMRP1183, Design 225
Proper citation: RRID:Addgene_211844 Copy
Species: Salmonella typhimurium
Genetic Insert: LRR-NEL
Vector Backbone Description: Backbone Marker:Diego Orzaez Lab; Backbone Size:6367; Vector Backbone:pDGB3_alpha1; Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:38446628
Comments: Rojas-Pierce Lab plasmid pMRP1145, Design 221
Proper citation: RRID:Addgene_211843 Copy
Species: Synthetic
Genetic Insert: LRR-NEL
Vector Backbone Description: Backbone Marker:Diego Orzaez Lab; Backbone Size:6367; Vector Backbone:pDGB3_alpha1; Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:38446628
Comments: Rojas-Pierce Lab plasmid pMRP1185, Design 241
Proper citation: RRID:Addgene_211842 Copy
Species: Salmonella typhimurium
Genetic Insert: LRR-NEL
Vector Backbone Description: Backbone Marker:Diego Orzaez Lab; Backbone Size:6367; Vector Backbone:pDGB3_alpha1; Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:38446628
Comments: Rojas-Pierce Lab plasmid pMRP1191, Design 224
Proper citation: RRID:Addgene_211841 Copy
Genetic Insert: mCherry-sfGFP
Vector Backbone Description: Vector Backbone:pGWB602; Vector Types:Bacterial Expression, Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:36705704
Proper citation: RRID:Addgene_213860 Copy
Species: Synthetic
Genetic Insert: Cepheid1b-ST
Vector Backbone Description: Backbone Marker:Addgene; Backbone Size:3619; Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37992174
Proper citation: RRID:Addgene_214967 Copy
Species: Synthetic
Genetic Insert: HR1b
Vector Backbone Description: Backbone Marker:Diego Orzaez Lab; Backbone Size:2105; Vector Backbone:pUPD2; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:38446628
Comments: Rojas-Pierce Lab plasmid pMRP1178
Proper citation: RRID:Addgene_211853 Copy
Species: E. coli, A. victoria
Genetic Insert: PancACe
Vector Backbone Description: Backbone Size:7300; Vector Backbone:pLJM1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39874963
Comments: Please visit https://doi.org/10.1101/2023.12.31.573774 for bioRxiv preprint.
Proper citation: RRID:Addgene_215708 Copy
Genetic Insert: CBE6b-SpCas9-2xUGI
Vector Backbone Description: Vector Backbone:pCMV with a BR322 origin; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38402281
Proper citation: RRID:Addgene_215820 Copy
Genetic Insert: CBE6d-SpCas9-2xUGI
Vector Backbone Description: Vector Backbone:pCMV with a BR322 origin; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38402281
Proper citation: RRID:Addgene_215822 Copy
Genetic Insert: CBE6a V106W-SpCas9-2xUGI
Vector Backbone Description: Vector Backbone:pCMV with a BR322 origin; Vector Types:in vitro Transcription Template; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38402281
Proper citation: RRID:Addgene_215824 Copy
Genetic Insert: CBE6d V106W-SpCas9-2xUGI
Vector Backbone Description: Vector Backbone:pCMV with a BR322 origin; Vector Types:in vitro Transcription Template; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38402281
Proper citation: RRID:Addgene_215827 Copy
Genetic Insert: CBE6c V106W-SpCas9-2xUGI
Vector Backbone Description: Vector Backbone:pCMV with a BR322 origin; Vector Types:in vitro Transcription Template; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38402281
Proper citation: RRID:Addgene_215826 Copy
Species: Salmonella typhimurium
Genetic Insert: LRR-NEL
Vector Backbone Description: Backbone Marker:Diego Orzaez Lab; Backbone Size:2105; Vector Backbone:pUPD2; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:38446628
Comments: Rojas-Pierce Lab plasmid pMRP1137
Proper citation: RRID:Addgene_211862 Copy
Species: Other
Genetic Insert: ER-TurboID-V5
Vector Backbone Description: Backbone Marker:PEL; Backbone Size:9019; Vector Backbone:pDest663; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags.
Proper citation: RRID:Addgene_214453 Copy
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