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Species: E. coli
Genetic Insert: RssB (R115A)
Vector Backbone Description: Backbone Size:3787; Vector Backbone:pOKD5; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30975721
Proper citation: RRID:Addgene_229472 Copy
Species: E. coli
Genetic Insert: plsB, plsC, cdsA, pssA
Vector Backbone Description: Backbone Size:3162; Vector Backbone:pUC57-oriL191-oriR194; Vector Types:Cell-free expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40471122
Comments: Please visit https://doi.org/10.1101/2024.03.22.586135 for bioRxiv preprint.
Proper citation: RRID:Addgene_216483 Copy
Species: E. coli
Genetic Insert: DH10β atpi:slr0214:gidB
Vector Backbone Description: Vector Backbone:n/a; Vector Types:; Bacterial Resistance:Streptomycin
Defining Citation: PMID:39846505
Comments: Strain has resistance to streptomycin but not spectinomycin, use spectinomycin 100 µg/mL for plasmids with a streptomycin resistance cassette.
This strain methylates the first cytosine in the palindrome CGATCG.
Proper citation: RRID:Addgene_226851 Copy
Species: E. coli
Genetic Insert: mitoEcSTH
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:9227; Vector Backbone:pLVX-TetOne-Puro; Vector Types:Mammalian Expression, Lentiviral, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37884806
Proper citation: RRID:Addgene_218629 Copy
Species: E. coli
Genetic Insert: EcNR1GT pUltraBR MjY dtyrS dtyrTV::tolC tyrU::gentR lambda::ZeoR
Vector Backbone Description: Vector Backbone:n/a; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin (50 ug/mL), Gentamycin (10 ug/mL)
Defining Citation: PMID:30078635
Proper citation: RRID:Addgene_218770 Copy
Species: E. coli
Genetic Insert: EcTyrRS-VSMA*
Vector Backbone Description: Backbone Size:4091; Vector Backbone:pEVOL-tac; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:38913984
Comments: EcY-tRNA-TAG
After the plasmids are propagated, they need to be gel purified by agarose gel because the ATM strains contain a plasmid in their genome.
Proper citation: RRID:Addgene_218766 Copy
Species: E. coli
Genetic Insert: EcWRS-h9
Vector Backbone Description: Backbone Size:4166; Vector Backbone:pEvoltac; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28192410
Comments: EcW-tRNA-TGA
After the plasmids are propagated, they need to be gel purified by agarose gel because the ATM strains contain a plasmid in their genome.
Proper citation: RRID:Addgene_218765 Copy
Species: E. coli
Genetic Insert: EcNR1GT pUltraBR MjY dtyrS tyrTV::inact tyrU::inact lambda::tolC
Vector Backbone Description: Vector Backbone:n/a; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30078635
Proper citation: RRID:Addgene_218773 Copy
Species: E. coli
Genetic Insert: C321.ΔA pUltraBR-MjY ΔtyrS tyrTV::tolC tyrU::GentR lambda::ZeoR
Vector Backbone Description: Vector Backbone:n/a; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin (50 ug/mL), Gentamycin (10 ug/mL)
Defining Citation: PMID:38913984
Proper citation: RRID:Addgene_218774 Copy
Species: E. coli
Genetic Insert: EcNR1GT pUltraBR MjY dtyrS tyrTV::CUA tyrU::inact lambda::tolC
Vector Backbone Description: Vector Backbone:n/a; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30078635
Proper citation: RRID:Addgene_218771 Copy
Species: E. coli
Genetic Insert: EcNR1GT pUltraBR MjY dtyrS tyrTV::CUA tyrU::CUA lambda::tolC
Vector Backbone Description: Vector Backbone:n/a; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30078635
Proper citation: RRID:Addgene_218772 Copy
Species: E. coli
Genetic Insert: speG
Vector Backbone Description: Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:38885650
Proper citation: RRID:Addgene_220483 Copy
Species: E. coli
Genetic Insert: IS621 bridge RNA
Vector Backbone Description: Vector Backbone:pNP555 - ColE1 Ori; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:38926615
Comments: Please visit https://doi.org/10.1101/2024.01.24.577089 for bioRxiv preprint.
Proper citation: RRID:Addgene_222725 Copy
Species: E. coli
Genetic Insert: IS621 Helper (lacZ)
Vector Backbone Description: Vector Backbone:pNP1950 - p15A Ori; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:38926615
Comments: Please visit https://doi.org/10.1101/2024.01.24.577089 for bioRxiv preprint.
Proper citation: RRID:Addgene_222733 Copy
Species: E. coli
Genetic Insert: ThrRS
Vector Backbone Description: Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40209036
Comments: It is best to grow cells in strains with LacIq and in media supplemented 1% glucose for genetic stability. Please visit https://doi.org/10.1101/2024.06.19.599772 for bioRxiv preprint.
Proper citation: RRID:Addgene_224365 Copy
Species: E. coli
Genetic Insert: AlaRS
Vector Backbone Description: Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40209036
Comments: It is best to grow cells in strains with LacIq and in media supplemented 1% glucose for genetic stability. Please visit https://doi.org/10.1101/2024.06.19.599772 for bioRxiv preprint.
Proper citation: RRID:Addgene_224362 Copy
Species: E. coli
Genetic Insert: EF-G
Vector Backbone Description: Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40209036
Comments: It is best to grow cells in strains with LacIq and in media supplemented 1% glucose for genetic stability. Please visit https://doi.org/10.1101/2024.06.19.599772 for bioRxiv preprint.
Proper citation: RRID:Addgene_224369 Copy
Species: E. coli
Genetic Insert: IF3
Vector Backbone Description: Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40209036
Comments: It is best to grow cells in strains with LacIq and in media supplemented 1% glucose for genetic stability. Please visit https://doi.org/10.1101/2024.06.19.599772 for bioRxiv preprint.
Proper citation: RRID:Addgene_224368 Copy
Species: E. coli
Genetic Insert: RF3
Vector Backbone Description: Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40209036
Comments: It is best to grow cells in strains with LacIq and in media supplemented 1% glucose for genetic stability. Please visit https://doi.org/10.1101/2024.06.19.599772 for bioRxiv preprint.
Proper citation: RRID:Addgene_224373 Copy
Species: E. Coli
Genetic Insert: Paph(3')-I_[abr]
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_225770 Copy
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