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Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 nsp6
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725302.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid.
Proper citation: RRID:Addgene_149215 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 ORF10 protein
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725255.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid.
Proper citation: RRID:Addgene_149219 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 ORF7a protein
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009724395.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid.
Proper citation: RRID:Addgene_149192 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 E (envelope)
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009724392.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid.
Proper citation: RRID:Addgene_149193 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 nsp6
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725302.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid.
Proper citation: RRID:Addgene_149195 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 M (membrane)
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009724393.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid.
Proper citation: RRID:Addgene_149185 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 nsp10
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725306.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid.
Proper citation: RRID:Addgene_149186 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 ORF10 protein
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725255.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid.
Proper citation: RRID:Addgene_149187 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 M (membrane)
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009724393.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid.
Proper citation: RRID:Addgene_149220 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 nsp9
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725305.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid.
Proper citation: RRID:Addgene_149189 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 endoRNAse
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725310.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid.
Proper citation: RRID:Addgene_149223 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Hygromycin resistant gene
Vector Backbone Description: Backbone Size:2517; Vector Backbone:pDONR221; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_139555 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: mCherry-CaaX
Vector Backbone Description: Backbone Size:1811; Vector Backbone:pGT; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32576833
Proper citation: RRID:Addgene_139500 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pSTL1 24xPP7sl
Vector Backbone Description: Backbone Size:3950; Vector Backbone:pHIS; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32576833
Comments: Addgene's lab has found that this plasmid encodes 22 PP7 stem loops rather than the reported 24. Depositor noted that PP7 stem loop repeat region is prone to losing repeats over time and recommends verifying repeats by whole plasmid sequencing to avoid further loss of repeats.
Proper citation: RRID:Addgene_139487 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pHSP12 24xPP7sl
Vector Backbone Description: Backbone Size:3950; Vector Backbone:pHIS; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32576833
Comments: Addgene's lab has found that this plasmid encodes 22 PP7 stem loops rather than the reported 24. The depositor has confirmed that this can happen over time and recommends using more stable bacterial cell strains and performing full plasmid sequencing of these constructs when sub-cloning and transforming to avoid further loss of repeats.
Proper citation: RRID:Addgene_139490 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pGRE2 24xPP7sl
Vector Backbone Description: Backbone Size:3950; Vector Backbone:pHIS; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32576833
Comments: Addgene's lab has found that this plasmid encodes 22 PP7 stem loops rather than the reported 24. Depositor noted that PP7 stem loop repeat region is prone to losing repeats over time and recommends verifying repeats by whole plasmid sequencing to avoid further loss of repeats.
Proper citation: RRID:Addgene_139491 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pADH1 PP7∆FG-GFPenvy tCYC1
Vector Backbone Description: Backbone Size:3861; Vector Backbone:pSIVURA; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32576833
Proper citation: RRID:Addgene_139494 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pTEF PP7∆FG-GFPenvy tCYC1
Vector Backbone Description: Backbone Size:3861; Vector Backbone:pSIVURA; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32576833
Proper citation: RRID:Addgene_139495 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: VPH1
Vector Backbone Description: Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32584255
Comments: Parental strain JK93da.
Proper citation: RRID:Addgene_140149 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SEC7
Vector Backbone Description: Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32584255
Comments: Parental strain JK93da.
Proper citation: RRID:Addgene_140151 Copy
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