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  • Organism:e. coli (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pAW216
 
Resource Report
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RRID:Addgene_113238 Cas1 E. coli Streptomycin PMID:28729350 Backbone Size:4000; Vector Backbone:pCDF1b; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin 2026-08-15 01:02:10 0
pAW156
 
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RRID:Addgene_113185 Cas1 E. coli Streptomycin PMID:28729350 Backbone Size:4000; Vector Backbone:pCDF1b; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin K259E 2026-08-15 01:02:09 0
pAW210
 
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RRID:Addgene_113189 Cas1 E. coli Streptomycin PMID:28729350 Backbone Size:4000; Vector Backbone:pCDF1b; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin H208A 2026-08-15 01:02:09 0
pAW188
 
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RRID:Addgene_113178 Cas1 E. coli Streptomycin PMID:28729350 Backbone Size:4000; Vector Backbone:pCDF1b; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin R131A 2026-08-15 01:02:09 0
pAW158
 
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RRID:Addgene_113177 Cas1 E. coli Streptomycin PMID:28729350 Backbone Size:4000; Vector Backbone:pCDF1b; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin R112E 2026-08-15 01:02:09 0
pAW152
 
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RRID:Addgene_113175 Cas1 E. coli Streptomycin PMID:28729350 Backbone Size:4000; Vector Backbone:pCDF1b; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin K12E 2026-08-15 01:02:09 0
pAW185
 
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RRID:Addgene_113179 Cas1 E. coli Streptomycin PMID:28729350 Backbone Size:4000; Vector Backbone:pCDF1b; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin R132A 2026-08-15 01:02:09 0
pAW189
 
Resource Report
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RRID:Addgene_113180 Cas1 E. coli Streptomycin PMID:28729350 Backbone Size:4000; Vector Backbone:pCDF1b; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin Q136A 2026-08-15 01:02:09 0
aav-SYN-tdNfsB(F124W)-mCherry (eNTR)
 
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RRID:Addgene_113760 tdNfsB(F124W)-mCherry E. coli Ampicillin PMID:30111097 Vector Backbone:AAV2; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin F124W 2026-08-15 01:02:15 0
aav-CAG-tdNfsB(F124W)-mCherry (eNTR)
 
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RRID:Addgene_113761 tdNfsB(F124W)-mCherry E. coli Ampicillin PMID:30111097 Vector Backbone:AAV2; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin F124W 2026-08-15 01:02:15 0
aav-SYN-FLEX-tdNfsB(F124W)-mCherry (eNTR)
 
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RRID:Addgene_113763 tdNfsB(F124W)-mCherry E. coli Ampicillin PMID:30111097 Sternson Lab recommendations for propagation of AAV-FLEX plasmids: "These vectors are prone to recombination. This is a well known issue with these AAV vectors and is due to the inverted terminal repeats (ITRs) required for AAV production. To minimize recombination, we propagate these plasmids in NEB Stable cells. Also, to minimize recombination, cells should be cultured at 30 C. Note that these cultures will grow slowly (20 h for minipreps). Better yields and culture times are obtained with 2xYT as the media. This is strongly recommended. Because recombination may still happen occasionally, we do a panel of restriction digestions to assess whether the ITRs are in tact. Separate digestions with PvuII, Sma1, and SnaB1 should be performed." Backbone Marker:Scott Sternson; Vector Backbone:AAV2; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin F124W 2026-08-15 01:02:15 0
psgRNAc
 
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1+ mentions
RRID:Addgene_114006 gRNA E. coli Chloramphenicol PMID:29765036 Vector Backbone:psgRNA; Vector Types:; Bacterial Resistance:Chloramphenicol 2026-08-15 01:02:18 2
pKSJ375
 
Resource Report
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RRID:Addgene_103064 colicin E1 immunity protein E. coli Ampicillin PMID:27795317 This protein was never expressed independently. It was cloned in order to subsequently clone a colicin gene, which would be lethal in the absence of the immunity protein. The cloned fragment was from base pair 500 to base pair 48 of ColE1, which encompasses the E1 immunity protein promoter and the gene. Backbone Marker:Novagen/EMD Millipore; Backbone Size:5227; Vector Backbone:pET52-b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:00:29 0
pMH-09
 
Resource Report
Resource Website
RRID:Addgene_158437 MotA E. coli Chloramphenicol PMID:28974721 Vector Backbone:pBAD33; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol 2026-08-15 01:08:17 0
pMH-25
 
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RRID:Addgene_158436 MotA E. coli Chloramphenicol PMID:28974721 Vector Backbone:pBAD33; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol 2026-08-15 01:08:16 0
pMH-06
 
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RRID:Addgene_158434 MotA E. coli Chloramphenicol PMID:28974721 Vector Backbone:pBAD33; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol 2026-08-15 01:08:17 0
pCMV Lex VP16 HA (P#1708)
 
Resource Report
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RRID:Addgene_14593 LexA E. coli Ampicillin PMID:15203006 Backbone Marker:Invitrogen; Backbone Size:5600; Vector Backbone:pcDNA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:07:02 0
pCMV Lex HA (P#1706)
 
Resource Report
Resource Website
RRID:Addgene_14591 LexA E. coli Ampicillin PMID:15203006 Backbone Marker:Invitrogen; Backbone Size:5600; Vector Backbone:pcDNA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:07:01 0
pAc5.1B-lambdaN-HA-MBP_D
 
Resource Report
Resource Website
RRID:Addgene_146120 MBP E. coli Ampicillin PMID:16581772 Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid. Vector Backbone:Unknown; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:07:04 0
pKDsgRNA-mqo
 
Resource Report
Resource Website
RRID:Addgene_154921 gRNA targeting mqo E. coli Spectinomycin PMID:33346713 Vector Backbone:pKD46; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Spectinomycin 2026-08-15 01:07:56 0

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