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Species: Homo sapiens
Genetic Insert: Caspase 9 catalytic mutant
Vector Backbone Description: Backbone Size:3666; Vector Backbone:pET-23 b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10085063
Proper citation: RRID:Addgene_11830 Copy
Species: Homo sapiens
Genetic Insert: CENP-A
Vector Backbone Description: Backbone Marker:MBL, Nagoya, Aichi, Japan; Backbone Size:4656; Vector Backbone:pmKO1-MC1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18778791
Comments: Bgl II-KpnI fragment of pEGFP-AF8 (Addgene plasmid # 117803) was recloned into BamHI and KpnI site of pmKO1-MC1.
Note that the cloning strategy adds additional amino acids onto the C-terminus of the insert (STVPELQQRIRETREQSLNKRPRL), but this does not interfere with plasmid function. The extra sequence includes an MCS for adding tags to the C-terminus of the insert.
Proper citation: RRID:Addgene_118224 Copy
Species: Homo sapiens
Genetic Insert: Caspase 3
Vector Backbone Description: Backbone Size:3666; Vector Backbone:pET-23 b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9065443
Proper citation: RRID:Addgene_11821 Copy
Species: Homo sapiens
Genetic Insert: Caspase 9
Vector Backbone Description: Backbone Size:3666; Vector Backbone:pET-23 b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10085063
Proper citation: RRID:Addgene_11829 Copy
Species: Homo sapiens
Genetic Insert: Caspase 8 minus Death Effecter Domain catalytic mutant
Vector Backbone Description: Backbone Size:3666; Vector Backbone:pET-15 b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9065443
Proper citation: RRID:Addgene_11828 Copy
Species: Homo sapiens
Genetic Insert: Caspase 6
Vector Backbone Description: Backbone Size:3666; Vector Backbone:pET-23 b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9065443
Proper citation: RRID:Addgene_11823 Copy
Species: Homo sapiens
Genetic Insert: Caspase 7
Vector Backbone Description: Backbone Size:3666; Vector Backbone:pET-23 b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9065443
Proper citation: RRID:Addgene_11825 Copy
Species: Homo sapiens
Genetic Insert: GAG FMLV fused to spCAS9
Vector Backbone Description: Vector Backbone:pCDNA3-like; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30604748
Comments: Please visit https://www.biorxiv.org/content/early/2017/10/12/202010 for BioRxiv preprint. Note, there are some additional amino acids on the 3' end of the insert--depositor confirmed this is the version used in the paper and the plasmid should function as described.
Proper citation: RRID:Addgene_119942 Copy
Species: Homo sapiens
Genetic Insert: PAX8
Vector Backbone Description: Backbone Marker:promega; Backbone Size:4818; Vector Backbone:pGL3-Basic; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_11994 Copy
Species: Homo sapiens
Genetic Insert: Osteopontin
Vector Backbone Description: Backbone Marker:promega; Backbone Size:4818; Vector Backbone:pGL3-Basic; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_11997 Copy
Species: Homo sapiens
Genetic Insert: G3BP1
Vector Backbone Description: Backbone Size:7000; Vector Backbone:pHAGE-UbiC; Vector Types:Mammalian Expression, Bacterial Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30661979
Comments: Please visit https://www.biorxiv.org/content/10.1101/332502v1 for BioRxiv preprint
Proper citation: RRID:Addgene_119949 Copy
Species: Homo sapiens
Genetic Insert: DDX6
Vector Backbone Description: Backbone Size:7000; Vector Backbone:pHAGE-UbiC; Vector Types:Mammalian Expression, Bacterial Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30661979
Comments: Please visit https://www.biorxiv.org/content/10.1101/332502v1 for BioRxiv preprint
Proper citation: RRID:Addgene_119947 Copy
Species: Homo sapiens
Genetic Insert: V5
Vector Backbone Description: Backbone Size:2412; Vector Backbone:pFA6a; Vector Types:PCR Template; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32406907
Comments: Template plasmid for C-terminal PCR tagging of mammalian genes. For M1 and M2 tagging oligo design please visit www.pcr-tagging.com Please visit https://www.biorxiv.org/content/early/2018/11/20/473876 for BioRxiv preprint
Proper citation: RRID:Addgene_120010 Copy
Species: Homo sapiens
Genetic Insert: S-Tag
Vector Backbone Description: Backbone Size:2412; Vector Backbone:pFA6a; Vector Types:PCR Template; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32406907
Comments: Template plasmid for C-terminal PCR tagging of mammalian genes. For M1 and M2 tagging oligo design please visit www.pcr-tagging.com Please visit https://www.biorxiv.org/content/early/2018/11/20/473876 for BioRxiv preprint
Proper citation: RRID:Addgene_120011 Copy
Species: Homo sapiens
Genetic Insert: TagBFP
Vector Backbone Description: Backbone Size:2412; Vector Backbone:pFA6a; Vector Types:PCR Template; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32406907
Comments: Template plasmid for C-terminal PCR tagging of mammalian genes. For M1 and M2 tagging oligo design please visit www.pcr-tagging.com Please visit https://www.biorxiv.org/content/early/2018/11/20/473876 for BioRxiv preprint
Proper citation: RRID:Addgene_120013 Copy
Species: Homo sapiens
Genetic Insert: TNKS2 3'UTR
Vector Backbone Description: Backbone Marker:pIS2 derived from pRL-SV40 (Promega); Backbone Size:3700; Vector Backbone:pIS2; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16308420
Comments: TNKS2 3'UTR in renilla luciferase reporter plasmid. Wild-type microRNA recognition sites (seed matches): 1760-1765 and 2254-2259.
Proper citation: RRID:Addgene_11999 Copy
Species: Homo sapiens
Genetic Insert: CORO1C 3'UTR
Vector Backbone Description: Backbone Marker:pIS2 derived from pRL-SV40 (Promega); Backbone Size:3700; Vector Backbone:pIS2; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16308420
Comments: CORO1C 3'UTR in renilla luciferase reporter plasmid. Wild-type microRNA recognition sites (seed matches): 1701-1706 and 1737-1742.
Proper citation: RRID:Addgene_11998 Copy
Species: Homo sapiens
Genetic Insert: mTFP1
Vector Backbone Description: Backbone Size:2412; Vector Backbone:pFA6a; Vector Types:PCR Template; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32406907
Comments: Template plasmid for C-terminal PCR tagging of mammalian genes. For M1 and M2 tagging oligo design please visit www.pcr-tagging.com Please visit https://www.biorxiv.org/content/early/2018/11/20/473876 for BioRxiv preprint
Proper citation: RRID:Addgene_120015 Copy
Species: Homo sapiens
Genetic Insert: 3xHA
Vector Backbone Description: Backbone Size:2412; Vector Backbone:pFA6a; Vector Types:PCR Template; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32406907
Comments: Template plasmid for C-terminal PCR tagging of mammalian genes. For M1 and M2 tagging oligo design please visit www.pcr-tagging.com Please visit https://www.biorxiv.org/content/early/2018/11/20/473876 for BioRxiv preprint
Proper citation: RRID:Addgene_120007 Copy
Species: Homo sapiens
Genetic Insert: Strep-II
Vector Backbone Description: Backbone Size:2412; Vector Backbone:pFA6a; Vector Types:PCR Template; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32406907
Comments: Template plasmid for C-terminal PCR tagging of mammalian genes. For M1 and M2 tagging oligo design please visit www.pcr-tagging.com Please visit https://www.biorxiv.org/content/early/2018/11/20/473876 for BioRxiv preprint
Proper citation: RRID:Addgene_120008 Copy
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