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Species: Candida auris
Genetic Insert: HYG 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, C. auris LEU2 2 of 2
Vector Backbone Description: Backbone Size:2242; Vector Backbone:pUC19; Vector Types:Bacterial Expression, Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34730409
Proper citation: RRID:Addgene_174433 Copy
Species: Synthetic
Genetic Insert: Candida optimized monomeric GFP
Vector Backbone Description: Backbone Size:2242; Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34730409
Proper citation: RRID:Addgene_174434 Copy
Species: Other
Genetic Insert: EPSUD
Vector Backbone Description: Vector Backbone:pHFX-B60; Vector Types:archaeal gene knockout vector for Haloferax mediterranei; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34433872
Proper citation: RRID:Addgene_174390 Copy
Species: Other
Genetic Insert: a crRNA targeting the template strand
Vector Backbone Description: Backbone Size:7876; Vector Backbone:pWL502; Vector Types:Bacterial Expression, CRISPR, archaeal expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34433872
Proper citation: RRID:Addgene_174383 Copy
Genetic Insert: pyrF-NT
Vector Backbone Description: Vector Backbone:pBM23; Vector Types:Bacterial Expression, Template for cloning fragments for chromosomal integration into Haloferax mediterranei; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34433872
Proper citation: RRID:Addgene_174384 Copy
Genetic Insert: pyrF
Vector Backbone Description: Vector Backbone:pMD18; Vector Types:Bacterial Expression, Template for cloning fragments for chromosomal integration into Haloferax mediterranei; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34433872
Proper citation: RRID:Addgene_174388 Copy
Species: Drosophila melanogaster
Genetic Insert: Drp1
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30531964
Proper citation: RRID:Addgene_174421 Copy
Species: Other
Genetic Insert: PAM-spacer
Vector Backbone Description: Backbone Size:7876; Vector Backbone:pWL502; Vector Types:Bacterial Expression, CRISPR, archaeal expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34433872
Proper citation: RRID:Addgene_174382 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL1 promoter + polylinker + CYC1 terminator
Vector Backbone Description: Backbone Marker:Sikorski & Hieter, Genetics 122:19-27 (1989); Backbone Size:2691; Vector Backbone:pRS406; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Comments: Mumberg D, Muller R, Funk M. Nucleic Acids Res. 25:5767-8 (1994).
Proper citation: RRID:Addgene_17437 Copy
Species: Rhabdomys pumilio
Genetic Insert: Centromere protein C
Vector Backbone Description: Vector Backbone:pIVT; Vector Types:in vitro transcription template; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34433012
Proper citation: RRID:Addgene_174446 Copy
Species: Mus musculus
Genetic Insert: JMJD3
Vector Backbone Description: Backbone Size:4400; Vector Backbone:pCS2+ NLSMT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18003914
Comments: Full length mouse JMJD3 with a C-terminal FLAG was PCR-amplified from MGC clone BC075632 and cloned into the XhoI-XbaI sites downstream of the CMV promoter and in frame with the N-terminal 6myc tag in the pCS2+ NLSMT vector.
Proper citation: RRID:Addgene_17440 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2349; Vector Backbone:pENTR1A; Vector Types:Mammalian Expression, Bacterial Expression, Yeast Expression, Insect Expression, Plant Expression, modified Gateway Entry vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18211686
Comments: Part of a modified Gateway Entry vector system for epitope tagging. Clone your gene of interest into this vector using the BamHI and NotI cloning sites to obtain a N-terminal STREP tagged Entry vector. The resulting Entry vector can be recombined into Gateway Destination vectors for bacteria, insect mammalian, plant or yeast expression via the Gateway LR reaction.
The tag is present in the Entry cassette to avoid the presence of att-derived protein linker sequences between the tag and gene or interest corresponding to the attB recombination site. A stop codon is already present within the vector (see plasmid map).
GenBank accession number: EU334816
Proper citation: RRID:Addgene_17441 Copy
Vector Backbone Description: Backbone Marker:Philippa Marrack Lab; Backbone Size:6063; Vector Backbone:MSCV-IRES-Thy1.1; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16873067
Comments: Recombination sites were added to MSCV-IRES-Thy1.1 for subcloning from pENTR vectors.
Proper citation: RRID:Addgene_17442 Copy
Species: Streptococcus pyogenes
Genetic Insert: Cas9 Endonuclease Dead
Vector Backbone Description: Vector Backbone:pIVT; Vector Types:in vitro transcription template; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34433012
Proper citation: RRID:Addgene_174442 Copy
Species: Homo sapiens
Genetic Insert: FOXP3
Vector Backbone Description: Backbone Size:6063; Vector Backbone:MSCV-IRES-Thy1.1 DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16873067
Proper citation: RRID:Addgene_17443 Copy
Genetic Insert: GFP
Vector Backbone Description: Backbone Size:8591; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19657394
Proper citation: RRID:Addgene_17446 Copy
Genetic Insert: GFP
Vector Backbone Description: Backbone Size:8068; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19657394
Proper citation: RRID:Addgene_17447 Copy
Species: Xenopus laevis
Genetic Insert: ePAB
Vector Backbone Description: Vector Backbone:pcDNA5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34213414
Proper citation: RRID:Addgene_174359 Copy
Species: Other
Genetic Insert: Sirius with golgi apparatus-targeting sequence
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pFX; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31735740
Proper citation: RRID:Addgene_174469 Copy
Species: Other
Genetic Insert: Sirius with endoplasmic reticulum-targeting sequence
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pFX; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31735740
Proper citation: RRID:Addgene_174464 Copy
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