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Species: Other
Genetic Insert: SECFP with endoplasmic reticulum-targeting sequence
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pFX; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31735740
Proper citation: RRID:Addgene_174465 Copy
Species: Other
Genetic Insert: protein A - hia5
Vector Backbone Description: Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35396487
Comments: The depositing laboratory recommends Addgene Plasmid 222303: prhaBAD-pA-Hia5, an improved version of this plasmid.
Protocol for purification and expression can be found at: dx.doi.org/10.17504/protocols.io.bv82n9ye
Application for DiMeLo-seq is described in: doi.org/10.1101/2021.07.06.451383
Protocol was adapted and modified from: doi.org/10.1126/science.aaz1646
Proper citation: RRID:Addgene_174372 Copy
Vector Backbone Description: Backbone Size:7373; Vector Backbone:pUC19; Vector Types:Vector for 2-step knockout by homologous recombination; Bacterial Resistance:Apramycin
Defining Citation: PMID:34890250
Comments: Also known as pSelAct. For cloning in homology arms, can use Gateway, restriction-ligation, or homology-based cloning such as Gibson/Infusion cloning. Please cite at minimum paper 1) and a secondary paper that is most appropriate to your system of interest (2 or 3).
1. First development of pSelAct for knockouts in Rhodococcus equi:
Geize, R. van der, Jong, W. de, Hessels, G. I., Grommen, A. W. F., Jacobs, A. A. C., and Dijkhuizen, L. 2008. A novel method to generate unmarked gene deletions in the intracellular pathogen Rhodococcus equi using 5-fluorocytosine conditional lethality. Nucleic Acids Res. 36:e151
2. First development of pSelAct with Gateway cassette for knockouts in Rhodococcus fasicans:
Savory, E. A., Weisberg, A. J., Stevens, D. M., Creason, A. L., Fuller, S. L., Pearce, E. M., and Chang, J. H. 2020. Phytopathogenic Rhodococcus Have Diverse Plasmids With Few Conserved Virulence Functions. Front Microbiol. 11:1022
3. Later referred to as pSelAct-KO after optimization for knockouts in Clavibacter:
Stevens, D. M., Tang, A., and Coaker, G. 2021. A Genetic Toolkit for Investigating Clavibacter Species: Markerless Deletion, Permissive Site Identification, and an Integrative Plasmid. Mol Plant-microbe Interactions. 34:1336–1345
Proper citation: RRID:Addgene_174374 Copy
Genetic Insert: TEV Protease
Vector Backbone Description: Vector Backbone:pB-CAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37053554
Proper citation: RRID:Addgene_174377 Copy
Genetic Insert: FLPo recombinase
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38212587
Proper citation: RRID:Addgene_174378 Copy
Genetic Insert: TEVP
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37053554
Proper citation: RRID:Addgene_174379 Copy
Species: Synthetic
Genetic Insert: RFP with PAM rich sequence upstream promoter
Vector Backbone Description: Backbone Size:3396; Vector Backbone:SC101; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33823546
Proper citation: RRID:Addgene_174369 Copy
Species: Candida auris
Genetic Insert: NAT 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, C. auris LEU2 2 of 2
Vector Backbone Description: Backbone Size:2242; Vector Backbone:pUC19; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34730409
Proper citation: RRID:Addgene_174405 Copy
Species: Candida auris
Genetic Insert: C. auris LEU2 1 of 2, pENO1, Cas9, NAT 1 of 2
Vector Backbone Description: Backbone Size:2242; Vector Backbone:pUC19; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34730409
Proper citation: RRID:Addgene_174409 Copy
Species: Streptomyces tendae
Genetic Insert: nikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
Vector Backbone Description: Backbone Size:3516; Vector Backbone:pCm No carrier; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:35101207
Proper citation: RRID:Addgene_174361 Copy
Species: Streptomyces tendae
Genetic Insert: nikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
Vector Backbone Description: Backbone Size:3831; Vector Backbone:pCm C-term SMT3; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:35101207
Proper citation: RRID:Addgene_174362 Copy
Species: Streptomyces tendae
Genetic Insert: nikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
Vector Backbone Description: Backbone Size:4710; Vector Backbone:pCm N-term MBP; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:35101207
Proper citation: RRID:Addgene_174364 Copy
Species: Streptomyces tendae
Genetic Insert: nikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
Vector Backbone Description: Backbone Size:4680; Vector Backbone:pCm C-term MBP; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:35101207
Proper citation: RRID:Addgene_174365 Copy
Species: Synthetic
Genetic Insert: Alpha N-terminal domain from the E. coli RNA polymerase fused to SYNZIP17
Vector Backbone Description: Backbone Size:1925; Vector Backbone:CloDF13; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33823546
Proper citation: RRID:Addgene_174367 Copy
Species: Salmonella enterica ser. Typhimurium
Genetic Insert: sarA
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31901521
Proper citation: RRID:Addgene_174400 Copy
Species: Salmonella enterica ser. Typhimurium
Genetic Insert: sarA
Vector Backbone Description: Vector Backbone:pWSK129; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31901521
Proper citation: RRID:Addgene_174401 Copy
Species: Lettuce, Rapeseed
Genetic Insert: DdCBE G1333-N
Vector Backbone Description: Backbone Size:4205; Vector Backbone:p3s; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34211132
Proper citation: RRID:Addgene_174556 Copy
Species: Lettuce, Rapeseed
Genetic Insert: DdCBE G1397-N
Vector Backbone Description: Backbone Size:4211; Vector Backbone:p3s; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34211132
Proper citation: RRID:Addgene_174558 Copy
Species: Lettuce, Rapeseed
Genetic Insert: DdCBE G1397-C
Vector Backbone Description: Backbone Size:4220; Vector Backbone:p3s; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34211132
Proper citation: RRID:Addgene_174559 Copy
Genetic Insert: Bcl2 and designed protein LD3
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34599176
Proper citation: RRID:Addgene_174550 Copy
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