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Species: Synthetic
Genetic Insert: BE3
Vector Backbone Description: Backbone Marker:Mumberg et al. (Nucleic Acids Research, 1994, Vol. 22, No. 25); Backbone Size:6653; Vector Backbone:p415-GALL; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36326816
Proper citation: RRID:Addgene_172409 Copy
Species: Synthetic
Genetic Insert: mPlum
Vector Backbone Description: Vector Backbone:pMax; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24757057
Comments: For more information, see Piett, Pecen et al. 2021 Nat Protoc. (doi: 10.1038/s41596-021-00577-3).
This plasmid has been found to be prone to multimerization. Multimerization often does not impact plasmid function, but it may reduce transformation efficiencies. If you need to isolate the monomeric version, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid.
Proper citation: RRID:Addgene_177830 Copy
Species: Synthetic
Genetic Insert: firefly luciferase
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32128416
Proper citation: RRID:Addgene_192634 Copy
Species: Synthetic
Genetic Insert: mScarlet
Vector Backbone Description: Vector Backbone:pLenti Trono; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34738089
Proper citation: RRID:Addgene_185665 Copy
Species: Synthetic
Genetic Insert: mScarlet
Vector Backbone Description: Vector Backbone:pLenti Trono; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34738089
Proper citation: RRID:Addgene_185662 Copy
Species: Synthetic
Genetic Insert: Ezr sgRNA
Vector Backbone Description: Backbone Marker:Khakh lab; Backbone Size:5206; Vector Backbone:PZac2.1; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36378959
Proper citation: RRID:Addgene_179119 Copy
Species: Synthetic
Genetic Insert: Fermt2 sgRNA
Vector Backbone Description: Backbone Marker:Khakh lab; Backbone Size:5206; Vector Backbone:PZac2.1; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36378959
Proper citation: RRID:Addgene_179117 Copy
Species: Synthetic
Genetic Insert: mScarlet
Vector Backbone Description: Vector Backbone:pLenti Trono; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34738089
Proper citation: RRID:Addgene_185666 Copy
Species: Synthetic
Genetic Insert: gRNA cassette
Vector Backbone Description: Backbone Marker:Mumberg et al. (Nucleic Acids Research, 1994, Vol. 22, No. 25); Backbone Size:6145; Vector Backbone:p426; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36326816
Proper citation: RRID:Addgene_172517 Copy
Species: Synthetic
Genetic Insert: LexA-CIB1-biLINuS
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32128416
Proper citation: RRID:Addgene_192605 Copy
Species: Synthetic
Genetic Insert: eGFP-P2A-CRY2PHR-VPR
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Lentiviral, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32128416
Proper citation: RRID:Addgene_192610 Copy
Species: Synthetic
Genetic Insert: G-Flamp1-mut
Vector Backbone Description: Backbone Size:6342; Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36097007
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.02.27.482140v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_188568 Copy
Species: Synthetic
Genetic Insert: NEMOf
Vector Backbone Description: Backbone Size:5222; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37081094
Comments: Please visit https://doi.org/10.1101/2022.08.23.504677 for bioRxiv preprint.
Proper citation: RRID:Addgene_189932 Copy
Species: Synthetic
Genetic Insert: NEMOc
Vector Backbone Description: Backbone Size:5222; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37081094
Comments: Please visit https://doi.org/10.1101/2022.08.23.504677 for bioRxiv preprint.
Proper citation: RRID:Addgene_189933 Copy
Species: Synthetic
Genetic Insert: LSSA12
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36344833
Comments: 398 nm excitation, 511 nm emission.
Proper citation: RRID:Addgene_186545 Copy
Species: Synthetic
Genetic Insert: Hyperfolder YFP (3X)
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36344833
Comments: Generates a fusion protein of three hfYFP molecules (3xhfYFP) for high cytosolic expression
Proper citation: RRID:Addgene_186544 Copy
Species: Synthetic
Genetic Insert: LSSmGFP
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36344833
Proper citation: RRID:Addgene_186543 Copy
Species: Synthetic
Genetic Insert: mGreenLantern
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36344833
Comments: To improve ER localization compared to Addgene #164463, the Kozak sequence and ATG that were N-terminal to the FP have been deleted.
Proper citation: RRID:Addgene_186548 Copy
Species: Synthetic
Genetic Insert: FOLD6
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pBAD/His-B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36344833
Comments: Backbone is identical to that of Addgene #37129 (several amino acids between the FP insert and His-tag are removed).
Proper citation: RRID:Addgene_186547 Copy
Species: Synthetic
Genetic Insert: LSSA12
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pBAD/His-B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36344833
Comments: 398 nm excitation, 511 nm emission. Backbone is identical to that of Addgene #37129 (several amino acids between the FP insert and His-tag are removed).
Proper citation: RRID:Addgene_186546 Copy
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