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Plasmids are provided by Addgene and DGRC.

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pFX-SECFP-Sec61β
 
Resource Report
Resource Website 		RRID:Addgene_174465 SECFP with endoplasmic reticulum-targeting sequence Other Kanamycin PMID:31735740 Backbone Size:4000; Vector Backbone:pFX; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:10:40 0
pET-pA-Hia5
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_174372 protein A - hia5 Other Kanamycin PMID:35396487 The depositing laboratory recommends Addgene Plasmid 222303: prhaBAD-pA-Hia5, an improved version of this plasmid. Protocol for purification and expression can be found at: dx.doi.org/10.17504/protocols.io.bv82n9ye Application for DiMeLo-seq is described in: doi.org/10.1101/2021.07.06.451383 Protocol was adapted and modified from: doi.org/10.1126/science.aaz1646 Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:10:39 1
pSelAct-KO
 
Resource Report
Resource Website 		RRID:Addgene_174374 Apramycin PMID:34890250 Also known as pSelAct. For cloning in homology arms, can use Gateway, restriction-ligation, or homology-based cloning such as Gibson/Infusion cloning. Please cite at minimum paper 1) and a secondary paper that is most appropriate to your system of interest (2 or 3). 1. First development of pSelAct for knockouts in Rhodococcus equi: Geize, R. van der, Jong, W. de, Hessels, G. I., Grommen, A. W. F., Jacobs, A. A. C., and Dijkhuizen, L. 2008. A novel method to generate unmarked gene deletions in the intracellular pathogen Rhodococcus equi using 5-fluorocytosine conditional lethality. Nucleic Acids Res. 36:e151 2. First development of pSelAct with Gateway cassette for knockouts in Rhodococcus fasicans: Savory, E. A., Weisberg, A. J., Stevens, D. M., Creason, A. L., Fuller, S. L., Pearce, E. M., and Chang, J. H. 2020. Phytopathogenic Rhodococcus Have Diverse Plasmids With Few Conserved Virulence Functions. Front Microbiol. 11:1022 3. Later referred to as pSelAct-KO after optimization for knockouts in Clavibacter:  Stevens, D. M., Tang, A., and Coaker, G. 2021. A Genetic Toolkit for Investigating Clavibacter Species: Markerless Deletion, Permissive Site Identification, and an Integrative Plasmid. Mol Plant-microbe Interactions. 34:1336–1345 Backbone Size:7373; Vector Backbone:pUC19; Vector Types:Vector for 2-step knockout by homologous recombination; Bacterial Resistance:Apramycin 2026-08-15 01:10:39 0
pB-CAG-TEVp-IRES-mCherry
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_174377 TEV Protease Ampicillin PMID:37053554 Vector Backbone:pB-CAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:10:39 1
pAAV-syn-Flpo
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_174378 FLPo recombinase Ampicillin PMID:38212587 Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin 2026-08-15 01:10:39 2
pAAV-TREtight-H2b-emiRFP670-P2A-TEVP
 
Resource Report
Resource Website 		RRID:Addgene_174379 TEVP Ampicillin PMID:37053554 Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin 2026-08-15 01:10:39 0
pJEC581
 
Resource Report
Resource Website 		RRID:Addgene_174369 RFP with PAM rich sequence upstream promoter Synthetic Kanamycin PMID:33823546 Backbone Size:3396; Vector Backbone:SC101; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin 2026-08-15 01:10:39 0
pCE27
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_174405 NAT 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, C. auris LEU2 2 of 2 Candida auris Ampicillin PMID:34730409 Backbone Size:2242; Vector Backbone:pUC19; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:10:40 2
pCE35
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_174409 C. auris LEU2 1 of 2, pENO1, Cas9, NAT 1 of 2 Candida auris Ampicillin PMID:34730409 Backbone Size:2242; Vector Backbone:pUC19; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:10:40 2
pCm No carrier NikJC199U
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_174361 nikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae] Streptomyces tendae Chloramphenicol PMID:35101207 Backbone Size:3516; Vector Backbone:pCm No carrier; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol C199U 2026-08-15 01:10:39 1
pCm C-term SMT3 NikJC199U
 
Resource Report
Resource Website 		RRID:Addgene_174362 nikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae] Streptomyces tendae Chloramphenicol PMID:35101207 Backbone Size:3831; Vector Backbone:pCm C-term SMT3; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol C199U 2026-08-15 01:10:39 0
pCm N-term MBP NikJC199U
 
Resource Report
Resource Website 		RRID:Addgene_174364 nikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae] Streptomyces tendae Chloramphenicol PMID:35101207 Backbone Size:4710; Vector Backbone:pCm N-term MBP; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol 2026-08-15 01:10:39 0
pCm C-term MBP NikJC199U
 
Resource Report
Resource Website 		RRID:Addgene_174365 nikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae] Streptomyces tendae Chloramphenicol PMID:35101207 Backbone Size:4680; Vector Backbone:pCm C-term MBP; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol C199U 2026-08-15 01:10:39 0
pJEC626
 
Resource Report
Resource Website 		RRID:Addgene_174367 Alpha N-terminal domain from the E. coli RNA polymerase fused to SYNZIP17 Synthetic Spectinomycin PMID:33823546 Backbone Size:1925; Vector Backbone:CloDF13; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin 2026-08-15 01:10:39 0
pEGFP-Flag-stm2585_L139*
 
Resource Report
Resource Website 		RRID:Addgene_174400 sarA Salmonella enterica ser. Typhimurium Kanamycin PMID:31901521 Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin L139* (Truncation after 139th amino acid in sarA) 2026-08-15 01:10:40 0
pWSK129-stm2585
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_174401 sarA Salmonella enterica ser. Typhimurium Kanamycin PMID:31901521 Vector Backbone:pWSK129; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:10:40 1
cp-DdCBE G1333-N
 
Resource Report
Resource Website 		RRID:Addgene_174556 DdCBE G1333-N Lettuce, Rapeseed Ampicillin PMID:34211132 Backbone Size:4205; Vector Backbone:p3s; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:10:41 0
cp-DdCBE G1397-N
 
Resource Report
Resource Website 		RRID:Addgene_174558 DdCBE G1397-N Lettuce, Rapeseed Ampicillin PMID:34211132 Backbone Size:4211; Vector Backbone:p3s; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:10:41 0
cp-DdCBE G1397-C
 
Resource Report
Resource Website 		RRID:Addgene_174559 DdCBE G1397-C Lettuce, Rapeseed Ampicillin PMID:34211132 Backbone Size:4220; Vector Backbone:p3s; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:10:41 0
S112
 
Resource Report
Resource Website 		RRID:Addgene_174550 Bcl2 and designed protein LD3 Ampicillin PMID:34599176 Backbone Size:6000; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:10:41 0

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