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Vector Backbone Description: Backbone Size:9617; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Mammalian Expression, Lentiviral, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:19657394
Proper citation: RRID:Addgene_17452 Copy
Genetic Insert: mdm2 and designed protein LD6
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34599176
Proper citation: RRID:Addgene_174551 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3313; Vector Backbone:pENTR1A; Vector Types:RNAi, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19657394
Proper citation: RRID:Addgene_17453 Copy
Vector Backbone Description: Backbone Size:10319; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Mammalian Expression, Lentiviral, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:19657394
Comments: Please note that there are several mismatches between depositor's sequence and Addgene's quality control sequence. These mismatches are in the backbone regions only and do not affect any of the features.
Proper citation: RRID:Addgene_17454 Copy
Species: Homo sapiens
Genetic Insert: ibcl2-v4, bcl2 inhibitor resistant protein
Vector Backbone Description: Backbone Size:600; Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34599176
Proper citation: RRID:Addgene_174553 Copy
Species: firefly
Genetic Insert: FKBP-Luciferase fusion
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2280; Vector Backbone:pENTR1A; Vector Types:Luciferase, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19657394
Proper citation: RRID:Addgene_17455 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2427; Vector Backbone:pENTR1A; Vector Types:Mammalian Expression, Bacterial Expression, Yeast Expression, Insect Expression, Plant Expression, modified Gateway Entry vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18211686
Comments: Part of a modified Gateway Entry vector system for generating epitope tagged constructs. Clone your gene of interest into this vector using the BamHI and NotI cloning sites to obtain a N-terminal 3x HA tagged Entry vector. The resulting Entry vector can be recombined into Gateway Destination vectors for bacteria, insect mammalian, plant or yeast expression via the Gateway LR reaction.
The tag is present in the Entry cassette to avoid the presence of att-derived protein linker sequences between the tag and gene or interest corresponding to the attB recombination site. A stop codon is already present within the vector (see plasmid map).
GenBank accession number: EU334817
Proper citation: RRID:Addgene_17456 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2595; Vector Backbone:pENTR1A; Vector Types:Mammalian Expression, Bacterial Expression, Yeast Expression, Insect Expression, Plant Expression, modified Gateway Entry vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18211686
Comments: Part of a modified Gateway Entry vector system for generating epitope tagged constructs. Clone your gene of interest into this vector using the BamHI and NotI cloning sites to obtain a N-terminal 6x MYC tagged Entry vector. The resulting Entry vector can be recombined into Gateway Destination vectors for bacteria, insect mammalian, plant or yeast expression via the Gateway LR reaction.
The tag is present in the Entry cassette to avoid the presence of att-derived protein linker sequences between the tag and gene or interest corresponding to the attB recombination site. A stop codon is already present within the vector (see plasmid map).
GenBank accession number: EU334818
Proper citation: RRID:Addgene_17457 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3030; Vector Backbone:pENTR1A; Vector Types:Mammalian Expression, Bacterial Expression, Yeast Expression, Insect Expression, Plant Expression, modified Gateway Entry vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18211686
Comments: Part of a modified Gateway Entry vector system for generating epitope tagged constructs. Clone your gene of interest into this vector using the BamHI and NotI cloning sites to obtain a N-terminal ECFP tagged Entry vector. The resulting Entry vector can be recombined into Gateway Destination vectors for bacteria, insect mammalian, plant or yeast expression via the Gateway LR reaction.
The tag is present in the Entry cassette to avoid the presence of att-derived protein linker sequences between the tag and gene or interest corresponding to the attB recombination site. A stop codon is already present within the vector (see plasmid map).
GenBank accession number: EU344820
Proper citation: RRID:Addgene_17459 Copy
Species: Caenorhabditis elegans
Genetic Insert: htz-1 targeting gRNA
Vector Backbone Description: Vector Backbone:pTK73; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34865044
Proper citation: RRID:Addgene_174546 Copy
Genetic Insert: BclxL and designed protein LD3
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34599176
Proper citation: RRID:Addgene_174549 Copy
Vector Backbone Description: Backbone Size:5067; Vector Backbone:pCWB; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34774798
Comments: The original pCWB-CMV mCherry plasmid was provided by the University of Michigan Vector Core. We inserted a cloning site into the original plasmid that would enable the cloning of a U6 promoter-driven sgRNA into the backbone.
Proper citation: RRID:Addgene_174540 Copy
Species: Lettuce, Rapeseed
Genetic Insert: DdCBE G1333-N
Vector Backbone Description: Backbone Size:4211; Vector Backbone:p3s; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34211132
Proper citation: RRID:Addgene_174560 Copy
Species: Lettuce, Rapeseed
Genetic Insert: DdCBE G1333-C
Vector Backbone Description: Backbone Size:4220; Vector Backbone:p3s; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34211132
Proper citation: RRID:Addgene_174561 Copy
Genetic Insert: GFP
Vector Backbone Description: Backbone Size:5728; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:19657394
Proper citation: RRID:Addgene_17449 Copy
Species: Homo sapiens
Genetic Insert: DCAF 15
Vector Backbone Description: Backbone Size:6845; Vector Backbone:pAC8; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31686031
Proper citation: RRID:Addgene_174479 Copy
Species: Homo sapiens
Genetic Insert: Ubiquitin
Vector Backbone Description: Backbone Size:3896; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
Defining Citation: PMID:34083482
Proper citation: RRID:Addgene_174517 Copy
Species: Other
Genetic Insert: Sirius with actin-targeting sequence
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pFX; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31735740
Proper citation: RRID:Addgene_174475 Copy
Species: Other
Genetic Insert: EGFP with actin-targeting sequence
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pFX; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31735740
Proper citation: RRID:Addgene_174477 Copy
Species: Synthetic
Genetic Insert: 3C-6xHIS
Vector Backbone Description: Vector Backbone:pUAP1; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Proper citation: RRID:Addgene_174590 Copy
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