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Species: Drosophila melanogaster
Genetic Insert: "spaghetti monster GFP-OLLAS"
Vector Backbone Description: Vector Backbone:pJFRC12-10XUAS-IVS-myr::GFP; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25964354
Proper citation: RRID:Addgene_63170 Copy
Species: Drosophila melanogaster
Genetic Insert: TGF-β activated kinase 1
Vector Backbone Description: Backbone Marker:lifetechnologies; Backbone Size:3500; Vector Backbone:pMT-V5-His; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_63753 Copy
Species: Drosophila melanogaster
Genetic Insert: Relish
Vector Backbone Description: Backbone Marker:lifetechnologies; Backbone Size:3500; Vector Backbone:pMT-V5-His; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_63750 Copy
Species: Drosophila melanogaster
Genetic Insert: immune deficiency
Vector Backbone Description: Backbone Marker:lifetechnologies; Backbone Size:3500; Vector Backbone:pMT-V5-His; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Comments: *Note: Addgene's quality control sequencing finds two amino acid residue substitutions, L47M and H212Y, in the Drosophila IMD insert. These residue changes are not thought to affect plasmid function, and the protein has been shown to activate the Imd pathway as expected.
Proper citation: RRID:Addgene_63748 Copy
Species: Drosophila melanogaster
Genetic Insert: SP-dCas9-VPR
Vector Backbone Description: Vector Backbone:pAWG; Vector Types:Insect Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25730490
Comments: Please note that this plasmid contains a stop codon between dCas9-VPR and eGFP and does NOT produce a GFP fusion protein.
Cas9 contains D839A and N863A mutations. Depositor states that these mutations should not affect function.
Proper citation: RRID:Addgene_63802 Copy
Species: Drosophila melanogaster
Genetic Insert: Wingless
Vector Backbone Description: Backbone Size:12500; Vector Backbone:pCaSpeR4; Vector Types:Drosophila Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18430724
Proper citation: RRID:Addgene_64321 Copy
Species: Drosophila melanogaster
Genetic Insert: dmBACCS2VL-IRES-dOrai-IRES-GFP
Vector Backbone Description: Backbone Marker:Modified from Clontech; Backbone Size:4581; Vector Backbone:pWPRE/C1 (modified pEGFP-C1); Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26282514
Comments: dmBACCS2VL is a slow recovery kinetic mutant of dmBACCS2.
Proper citation: RRID:Addgene_72897 Copy
Species: Drosophila melanogaster
Genetic Insert: dmBACCS2-IRES-dOrai-IRES-mCherry
Vector Backbone Description: Backbone Marker:Modified from Clontech; Backbone Size:4581; Vector Backbone:pWPRE/C1 (modified pEGFP-C1); Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26282514
Proper citation: RRID:Addgene_72894 Copy
Species: Drosophila melanogaster
Genetic Insert: dmBACCS2NS-IRES-dOrai-IRES-GFP
Vector Backbone Description: Backbone Marker:Modified from Clontech; Backbone Size:4581; Vector Backbone:pWPRE/C1 (modified pEGFP-C1); Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26282514
Comments: dmBACCS2NS is a fast recovery kinetic mutant of dmBACCS2.
Proper citation: RRID:Addgene_72895 Copy
Species: Drosophila melanogaster
Genetic Insert: Orco (Y478A)
Vector Backbone Description: Vector Backbone:pUAST attB; Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22403649
Proper citation: RRID:Addgene_73928 Copy
Species: Drosophila melanogaster
Genetic Insert: dU6-3:tRNA:gRNA:tRNA:gRNA
Vector Backbone Description: Backbone Marker:Norbert Perrimon, Jian-Quan Ni, Harvard; Vector Backbone:pValium22; Vector Types:Insect Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27595403
Comments: Please acknowledge Fillip Port and Simon Bullock when publishing work derived from use of this plasmid.
Visit crisprflydesign.org for more information.
Proper citation: RRID:Addgene_73914 Copy
Species: Drosophila melanogaster
Genetic Insert: crumbs
Vector Backbone Description: Backbone Size:8904; Vector Backbone:pUAST; Vector Types:Insect Expression, Drosophila; Bacterial Resistance:Ampicillin
Comments: The plasmid contains part of the 5' cDNA of Crb and the 3' part that brings the transmembrane and the intracellular domains of the protein together. The BglII restriction site links the 5' and 3' regions of the cDNA, meaning that the majority of the extracellular part of the Crb protein is missing. The fragment also contains the 5' and 3' UTR regions. BglII restriction site was used to clone a GFP fragment. GFP is inserted between aa 96 and 2122 of crb. Crb contains K89E mutation which does not affect plasmid function.
Proper citation: RRID:Addgene_67525 Copy
Species: Drosophila melanogaster
Genetic Insert: dyl - chitin binding domain - tdTomato - dyl
Vector Backbone Description: Backbone Marker:Norbert Perrimon; Backbone Size:7541; Vector Backbone:pWalium-moe; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26395478
Comments: The plasmid contains pieces of DNA from a number of different sources. The td-Tomato was obtained from FUtdTW, Addgene.
Chitin binding domain was purchased from New England Biolabs
The dyl cDNA came from the Drosophila genome project
The pWalium10 moe vector came originally from the N. Perrimon Lab.
Proper citation: RRID:Addgene_67756 Copy
Species: Drosophila melanogaster
Genetic Insert: dU6-2:gRNA
Vector Backbone Description: Backbone Marker:Norbert Perrimon, Jian-Quan Ni, Harvard; Vector Backbone:pValium22; Vector Types:Insect Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25002478
Comments: Please acknowledge Fillip Port and Simon Bullock when publishing work derived from use of this plasmid.
Visit crisprflydesign.org for more information.
Proper citation: RRID:Addgene_49409 Copy
Species: Drosophila melanogaster
Genetic Insert: dU6-1:gRNA
Vector Backbone Description: Backbone Marker:Norbert Perrimon, Jian-Quan Ni, Harvard; Vector Backbone:pValium22; Vector Types:Insect Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25002478
Comments: Please acknowledge Fillip Port and Simon Bullock when publishing work derived from use of this plasmid.
Visit crisprflydesign.org for more information.
Proper citation: RRID:Addgene_49408 Copy
Species: Drosophila melanogaster
Genetic Insert: U6.3-gRNA[y]-scaffold
Vector Backbone Description: Backbone Size:3500; Vector Backbone:piggyBac; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34661429
Proper citation: RRID:Addgene_168294 Copy
Species: Drosophila melanogaster
Genetic Insert: Drosophila Chibby
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2P+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12712206
Comments: Description: The full coding sequence of Drosophila Chibby, 0.3kb, was amplified by PCR and subcloned into the BamH1 and EcoR1 sites of pCS2P+.
Reference: K. Takemaru et al., Nature 422: 905 (2003)
Proper citation: RRID:Addgene_16822 Copy
Species: Drosophila melanogaster
Genetic Insert: armadillo
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript KS (+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:2261639
Comments: Description: Full coding sequence of Drosophila armadillo in pBluescript (+)KS'.
T7 primer is at the 5' end, followed by BamHI site at nt 150 of arm cDNA, and the initiation ATG is 6-8nt downstream. There is an EcoRI site at nt 287 of the arm cDNA, and a stop at nt 2704, followed by an Eco RI site at nt 2770 and a Bam HI at 2820. Thus the insert can best be obtained with a BamHI digest.
Proper citation: RRID:Addgene_16829 Copy
Species: Drosophila melanogaster
Genetic Insert: calx2
Vector Backbone Description: Backbone Size:8780; Vector Backbone:pCaspeRhs; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15694324
Proper citation: RRID:Addgene_1741 Copy
Species: Drosophila melanogaster
Genetic Insert: Drp1
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30531964
Proper citation: RRID:Addgene_174421 Copy
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