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Species: Mus musculus
Genetic Insert: p53DD
Vector Backbone Description: Backbone Size:1600; Vector Backbone:pENTR4; Vector Types:in vitro transcription; Bacterial Resistance:Kanamycin
Defining Citation: PMID:42062565
Comments: The 5' and 3' beta globin UTR sequences flanking the ORF were based on previous literature for effective modRNA expression in hiPSCs: 10.1016/j.crmeth.2022.100290
To generate linear IVT template, digest the plasmid with MluI. The vector encodes a segmented polyA track, obviating the need for polyadenylation after IVT (doi: 10.1261/rna.069286.118).
Due to the inability of nanopore sequencing to accurately read long polyN stretches and the possibility of polyA truncation, the actual length of the polyA tail may differ when propagating the vector. In our hands, we have not seen measurable differences in expression across different plasmid preparations.
Proper citation: RRID:Addgene_232751 Copy
Species: Mus musculus
Genetic Insert: mouse homeostatic iron regulator with H67D mutation
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_233040 Copy
Species: Mus musculus
Genetic Insert: ITGA5
Vector Backbone Description: Vector Backbone:pLentiCRISPR V2 Neo; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37577760
Proper citation: RRID:Addgene_233251 Copy
Species: Mus musculus
Genetic Insert: ITGA5
Vector Backbone Description: Vector Backbone:pLentiCRISPR V2 Neo; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37577760
Proper citation: RRID:Addgene_233252 Copy
Species: Mus musculus
Genetic Insert: ITGA5
Vector Backbone Description: Vector Backbone:pLentiCRISPR V2 Neo; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37577760
Proper citation: RRID:Addgene_233253 Copy
Species: Mus musculus
Genetic Insert: ITGB1
Vector Backbone Description: Vector Backbone:pLentiCRISPR V2 Neo; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37577760
Proper citation: RRID:Addgene_233250 Copy
Species: Mus musculus
Genetic Insert: ITGB1
Vector Backbone Description: Vector Backbone:pLentiCRISPR V2 Neo; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37577760
Proper citation: RRID:Addgene_233249 Copy
Species: Mus musculus
Genetic Insert: Flag-Halo-CTCF WT
Vector Backbone Description: Backbone Marker:Benoit Bruneau (Addgene #156432); Vector Backbone:pEN366; Vector Types:Mammalian Expression, Mouse Targeting; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_232930 Copy
Species: Mus musculus
Genetic Insert: Etfdh
Vector Backbone Description: Vector Backbone:pUltra; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Comments: Please see Addgene plasmid #24129 for pUltra cloning details.
Please visit https://doi.org/10.1101/2024.10.25.620155 for bioRxiv preprint.
Proper citation: RRID:Addgene_232316 Copy
Species: Mus musculus
Genetic Insert: Crls1
Vector Backbone Description: Backbone Marker:Feng Zhang, Addgene plasmid #52961; Vector Backbone:lentiCRISPRv2; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37924812
Proper citation: RRID:Addgene_234766 Copy
Species: Mus musculus
Genetic Insert: Airn
Vector Backbone Description: Vector Backbone:pBACe3.6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40118040
Proper citation: RRID:Addgene_233266 Copy
Species: Mus musculus
Genetic Insert: Mex3a
Vector Backbone Description: Vector Backbone:pTRE2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Please note, this mutantRING version of Mex3a preserves mus musculus amino acids for Mex3a (except the four amino acids of RING domain's cross brace structure), but changes the underlying DNA sequence to reduce CG content.
Proper citation: RRID:Addgene_223245 Copy
Species: Mus musculus
Genetic Insert: sesRNA for mouse Rorb
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36198803
Proper citation: RRID:Addgene_239029 Copy
Species: Mus musculus
Genetic Insert: sesRNA for mouse Fezf2
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36198803
Proper citation: RRID:Addgene_239027 Copy
Species: Mus musculus
Genetic Insert: iGlucoSnFR.L276V.K312A
Vector Backbone Description: Vector Backbone:pLEX; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40120584
Proper citation: RRID:Addgene_238945 Copy
Species: Mus musculus
Genetic Insert: FLPo
Vector Backbone Description: Backbone Size:3365; Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_236288 Copy
Species: Mus musculus
Genetic Insert: alpha-MSH
Vector Backbone Description: Backbone Marker:Vector Builder; Vector Backbone:pAAV[FLEXon]; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37171957
Proper citation: RRID:Addgene_239044 Copy
Species: Mus musculus
Genetic Insert: Rab34
Vector Backbone Description: Vector Backbone:pEGFPc1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31209342
Proper citation: RRID:Addgene_237338 Copy
Species: Mus musculus
Genetic Insert: Rab23
Vector Backbone Description: Vector Backbone:pEGFPc1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31209342
Proper citation: RRID:Addgene_237337 Copy
Species: Mus musculus
Genetic Insert: Sall4
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript II KS-; Vector Types:Mouse Targeting; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_235510 Copy
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