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Species: Rattus norvegicus
Genetic Insert: NR1
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4006; Vector Backbone:pCI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12160751
Proper citation: RRID:Addgene_45446 Copy
Species: Rattus norvegicus
Genetic Insert: NR2A
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4006; Vector Backbone:pCI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12160751
Proper citation: RRID:Addgene_45445 Copy
Species: Escherichia coli
Genetic Insert: rpoA
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3993; Vector Backbone:pGEMEX1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23389035
Proper citation: RRID:Addgene_45398 Copy
Species: Bos taurus
Genetic Insert: Rhodopsin
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22855808
Proper citation: RRID:Addgene_45399 Copy
Species: Synthetic
Genetic Insert: AcrB/AcrD/AcrFa, ABO_0964
Vector Backbone Description: Vector Backbone:pBbA5k; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21556065
Comments: Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_45434 Copy
Species: Macaca mulatta (Rhesus monkey)
Genetic Insert: MAVS
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pFLAG-CMV2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22427742
Proper citation: RRID:Addgene_45372 Copy
Species: Mus musculus
Genetic Insert: PKA regulatory subunit RI beta tagged by mEGFP
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19447092
Proper citation: RRID:Addgene_45526 Copy
Species: Mus musculus
Genetic Insert: TRPM7
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5000; Vector Backbone:pcDNA4/TO (modified); Vector Types:Mammalian Expression, Tetracycline Inducible; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11385574
Comments: For the purpose of expressing LTRPC7 in eukaryotic cells, the depositing lab used PCR to produce an epitope tagged expression construct from two overlapping murine LTRPC7 clones. The LTRPC7 coding sequence was modified by removing the initiating methionine and replacing it with a sequence encoding a Kozak sequence, the FLAG tag and the additional sequence GCGGCCGCAT, and by placing a SpeI site just after the stop codon. These modifications result in an expressed protein which started with the following amino acid sequence: MGDYKDDDDKRPH followed by the murine LTRPC7 coding sequence starting at the second amino acid. This construct is expressed from the pcDNA4/TO vector which provides tetracycline-controlled expression from a CMV promotor.
Proper citation: RRID:Addgene_45482 Copy
Species: Mus musculus
Genetic Insert: c-Myc
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7143; Vector Backbone:pcDNA DEST40; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15048125
Comments: Alternate plasmid names: pD40-His-c-Myc; pcDNA-DEST40 Myc wt
This His6-tagged c-Myc plasmid was created by PCR amplification of the coding sequence for wild-type murine c-Myc2 from the CMVMyc plasmid (Hann et al., Genes Dev. 1994, PMID: 7958908) with a CACC 5′ extension added to the 5′ primer for cloning into the TOPO entry clone from Invitrogen (Carlsbad, CA). This sequence was then moved into the pDEST-40 mammalian expression vector using Gateway technology (Invitrogen), creating pD40-His-c-Myc.
For plasmid usage information, see the associated article (Yeh et al. Nat Cell Biol. 2004) as well as Arnold et al. EMBO 2009 (PMID: 19131971).
Proper citation: RRID:Addgene_45597 Copy
Species: Rhodopseudomonas palustris
Genetic Insert: iRFP670
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4055; Vector Backbone:pN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23770755
Proper citation: RRID:Addgene_45466 Copy
Species: Rhodopseudomonas palustris
Genetic Insert: iRFP713
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4038; Vector Backbone:pN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23770755
Comments: For more information on in vivo imaging, please see:
https://www.addgene.org/fluorescent_proteins/in_vivo/
Proper citation: RRID:Addgene_45465 Copy
Species: Mus musculus
Genetic Insert: PGC-1alpha Isoform1
Vector Backbone Description: Backbone Size:5424; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23217713
Comments: Note that Addgene NGS results identified a Q339K mutation relative to the canonical GenBank sequence. This mutation has not been reported to affect function.
Proper citation: RRID:Addgene_45501 Copy
Species: Rhodopseudomonas palustris
Genetic Insert: iRFP720
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4067; Vector Backbone:pBAD/HisB; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23770755
Proper citation: RRID:Addgene_45460 Copy
Species: Rhodopseudomonas palustris
Genetic Insert: iRFP670
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4038; Vector Backbone:pN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23770755
Proper citation: RRID:Addgene_45462 Copy
Genetic Insert: co I-Sce I
Vector Backbone Description: Backbone Size:5071; Vector Backbone:pCVL; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24121685
Proper citation: RRID:Addgene_45574 Copy
Species: Homo sapiens
Genetic Insert: eGFP with I-Sce I TS
Vector Backbone Description: Backbone Size:4483; Vector Backbone:pCVL; Vector Types:Mammalian Expression, Bacterial Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24121685
Proper citation: RRID:Addgene_45575 Copy
Species: Homo sapiens
Genetic Insert: TRIM28
Vector Backbone Description: Backbone Marker:Amersham GE Healthcare; Backbone Size:5000; Vector Backbone:pGEX-5X; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21940674
Comments: Full-length human TRIM28 was purchased from Origene and subcloned into vector pKH3 to generate pKH3-Trim28 (Addgene plasmid #45569). A full length TRIM28 DNA fragment was then generated from pKH3-TRIM28 by PCR and cloned into pGEX-5X.
Proper citation: RRID:Addgene_45570 Copy
Genetic Insert: FRT-cat-FRT
Vector Backbone Description: Backbone Marker:M. Koob (University of Wisconsin, Madison); Vector Backbone:pANTSγ; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:10829079
Comments: This is a template plasmids for frt-flanked cat cassette. The cat cassette originally came from pSC140.
pKD3 and pKD4 are identical except for the region between the FRT sites, so they create an identical scar that has stop codons in all six reading frames. As drawn in the map, this scar has an idealized ribosome binding site and start codon for downstream (rightward) gene expression.
Proper citation: RRID:Addgene_45604 Copy
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5729; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Plasmid pIRES-EGFP-puro was constructed containing the Phcmv promoter followed by unique restriction sites (NheI, XhoI, and SacI) for insertion of genes. Downstream, an IRES from the encephalomyocarditis virus directs the translation of EGFP-puro from the same mRNA.
Addgene's sequencing results found differences compared to the depositing lab's sequence in the 3' region of the puro resistance marker. The resulting protein sequence matches NCBI WP_055528321.1 and should be functional.
Proper citation: RRID:Addgene_45567 Copy
Species: Homo sapiens
Genetic Insert: TRIM28
Vector Backbone Description: Backbone Marker:Addgene plasmid Plasmid 12555; Backbone Size:4800; Vector Backbone:pKH3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21940674
Comments: Full-length human TRIM28 was purchased from Origene and subcloned into vector pKH3 to generate pKH3-Trim28.
Proper citation: RRID:Addgene_45569 Copy
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