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Species: Other
Genetic Insert: pKar2-msGFP-HDEL
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30314480
Comments: Integration plasmid in the His4 locus. Please note that there are some sequence discrepancies between the Addgene quality control sequence and the author-supplied Genbank sequence. The authors have noted that the discrepancies do NOT affect plasmid function.
Proper citation: RRID:Addgene_117668 Copy
Species: Other
Genetic Insert: pre-Ost1-alphaf(I)-Btl2
Vector Backbone Description: Vector Backbone:pPICZalpha; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:30314480
Comments: Integration plasmid in the pAOX1 locus.
Proper citation: RRID:Addgene_117667 Copy
Species: Synthetic
Genetic Insert: sfCherry2(1-10)
Vector Backbone Description: Backbone Size:5420; Vector Backbone:pETDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31552297
Comments: Please visit https://www.biorxiv.org/content/10.1101/454041v1 for BioRxiv preprint
Proper citation: RRID:Addgene_117656 Copy
Species: Escherichia coli
Genetic Insert: Translation factor cistrons 22-30
Vector Backbone Description: Backbone Size:5018; Vector Backbone:BioBrick pET-24a(+); Vector Types:Escherichia coli; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28977654
Comments: The plasmid can be prone to recombination. The depositing lab suggests selecting multiple clones for restriction digest analysis as described in the publication prior to proceeding.
Proper citation: RRID:Addgene_117762 Copy
Species: Escherichia coli
Genetic Insert: Translation factor cistrons 14-21
Vector Backbone Description: Backbone Size:5018; Vector Backbone:BioBrick pET-24a(+); Vector Types:Escherichia coli; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28977654
Comments: The plasmid can be prone to recombination. The depositing lab suggests selecting multiple clones for restriction digest analysis as described in the publication prior to proceeding.
Proper citation: RRID:Addgene_117761 Copy
Species: Escherichia coli
Genetic Insert: Translation factor cistrons 1-13
Vector Backbone Description: Backbone Size:5018; Vector Backbone:BioBrick pET-24a(+); Vector Types:Escherichia coli; Bacterial Resistance:Tetracyline and Kanamycin
Defining Citation: PMID:28977654
Comments: The plasmid can be prone to recombination. The depositing lab suggests selecting multiple clones for restriction digest analysis as described in the publication before proceeding.
Proper citation: RRID:Addgene_117760 Copy
Species: Homo sapiens
Genetic Insert: htt 25Q
Vector Backbone Description: Backbone Size:0; Vector Backbone:p416 GPD; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10677504
Comments: N terminal region of Ht with polyQ repeat length of 25 fused to GFP.
Proper citation: RRID:Addgene_1177 Copy
Species: Homo sapiens
Genetic Insert: htt 46Q
Vector Backbone Description: Backbone Size:0; Vector Backbone:p416 GPD; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10677504
Comments: N terminal region of Ht with polyQ repeat length of 46 fused to GFP.
Proper citation: RRID:Addgene_1178 Copy
Species: Synthetic
Genetic Insert: anti-GFP nanobody fused to a T7, tev site, mCherry, HA, BAP and His6 epitope
Vector Backbone Description: Backbone Marker:Merck - Novagen; Vector Backbone:pET24a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30855580
Proper citation: RRID:Addgene_117752 Copy
Species: Pectobacterium carotovorum
Genetic Insert: Codon optimised Carbapenem synthase (CarC), Carboxymethylproline synthase (CarB), Carbapenam synthetase (CarA), Ferredoxin CarE
Vector Backbone Description: Backbone Marker:Genebank; Vector Backbone:pBbaA5k; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29942079
Proper citation: RRID:Addgene_117755 Copy
Species: Mus musculus
Genetic Insert: ALK6
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9748228
Proper citation: RRID:Addgene_11769 Copy
Species: Homo sapiens
Genetic Insert: RASH1
Vector Backbone Description: Backbone Marker:Broad Institute; Vector Backbone:pLX311; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30777879
Proper citation: RRID:Addgene_117749 Copy
Species: Homo sapiens
Genetic Insert: DEDD1
Vector Backbone Description: Backbone Marker:Broad Institute; Backbone Size:9879; Vector Backbone:pLX311; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30777879
Proper citation: RRID:Addgene_117745 Copy
Species: Homo sapiens
Genetic Insert: DEDD1
Vector Backbone Description: Backbone Marker:Broad Institute; Backbone Size:10065; Vector Backbone:pLX307; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30777879
Proper citation: RRID:Addgene_117744 Copy
Vector Backbone Description: Backbone Size:4168; Vector Backbone:pPTbsr; Vector Types:Vector for Phaeodactylum tricornutum; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30488015
Proper citation: RRID:Addgene_117696 Copy
Species: Oryza sativa
Genetic Insert: TRANSPORT INHIBITOR RESPONSE 1
Vector Backbone Description: Backbone Marker:Francis Stewart; Backbone Size:6399; Vector Backbone:custom; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30120238
Comments: The codon optimized NLS-TIR1 is described in:
Baker, O. et al. RAC-tagging: Recombineering And Cas9-assisted targeting for protein tagging and conditional analyses. Sci Rep 6, 25529 (2016).
Some ambiguous bases and differences are present between the depositor sequence and Addgene's NGS sequence, but they are not expected to impact plasmid functionality.
Proper citation: RRID:Addgene_117699 Copy
Species: Synthetic
Genetic Insert: hcRed1
Vector Backbone Description: Backbone Marker:Broad Institute; Vector Backbone:pLX307; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30777879
Proper citation: RRID:Addgene_117732 Copy
Species: Bos taurus
Genetic Insert: BARKrgs peptide
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34139149
Proper citation: RRID:Addgene_117693 Copy
Species: Bos taurus
Genetic Insert: BARKrgs D110A peptide
Vector Backbone Description: Vector Backbone:pZac2.1; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34139149
Proper citation: RRID:Addgene_117692 Copy
Vector Backbone Description: Backbone Marker:pYUB1062 was donated by William R. Jacobs from the Department of Microbiology and Immunology at the Albert Einstein; Backbone Size:5800; Vector Backbone:pYUB1062; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:30319771
Comments: This is a shuttle vector, designed for expression of the inserted gene in Mycobacterium smegmatis as well as allowing cloning and expressions using E. coli.
This plasmid was constructed by Anajana Radhakrishnan from the pYUB1062 vector. pYUB1062 was donated by William R. Jacobs from the Department of Microbiology and Immunology at the Albert Einstein College of Medicine, New York, USA.
References: this paper has been accepted in RSC Advances:
A GFP-strategy for efficient recombinant protein overexpression and purification in Mycobacterium smegmatis: Anjana Radhakrishnan, Christopher M Furze, Mohd Syed Ahangar, Elizabeth Fullam
Proper citation: RRID:Addgene_117695 Copy
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