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Species: Homo sapiens
Genetic Insert: Human CYP3A4 proximal enhancer sgRNA for activation
Vector Backbone Description: Vector Backbone:lenti-sgRNA(MS2)_zeo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35452598
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.03.24.485641v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_176839 Copy
Species: Rattus norvegicus
Genetic Insert: T alpha 1p
Vector Backbone Description: Backbone Size:0; Vector Backbone:na; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17721509
Proper citation: RRID:Addgene_17707 Copy
Species: Homo sapiens
Genetic Insert: rent1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12228722
Proper citation: RRID:Addgene_17708 Copy
Vector Backbone Description: Backbone Size:7600; Vector Backbone:pCLE; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10839358
Comments: pCLE includes a modified 5' LTR (CMV/MLV) to obtain higher titers, as well as the Xenopus EF1alpha enhancer/promoter to improve vector expression in the brain. In addition, the vector is dicistronic and utilizes an IRES to allow translation of the reporter gene, human placental alkaline phosphatase (PLAP).
Proper citation: RRID:Addgene_17703 Copy
Genetic Insert: CBFRE
Vector Backbone Description: Backbone Size:3600; Vector Backbone:na; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17721509
Comments: CBFRE: four CBF1-binding elements from EBV (see Hsieh MCB 1996, 16:952) and the basal simian virus 40 (SV40) promoter upstream of EGFP. The sequence of the CBF1 binding element is GATCTGGAGTAACACGCCGTGGGAAAAAATTTATG. The binding site within this sequence is GTGGGAA.
A SmaI+XbaI double digest should yield 3.6kb and approx. 1.2kb bands. See Reviews link in right-hand column to view Addgene's diagnostic digest of this plasmid.
Proper citation: RRID:Addgene_17705 Copy
Vector Backbone Description: Backbone Size:9617; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Mammalian Expression, Lentiviral, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:19657394
Proper citation: RRID:Addgene_17452 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3313; Vector Backbone:pENTR1A; Vector Types:RNAi, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19657394
Proper citation: RRID:Addgene_17453 Copy
Vector Backbone Description: Backbone Size:10319; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Mammalian Expression, Lentiviral, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:19657394
Comments: Please note that there are several mismatches between depositor's sequence and Addgene's quality control sequence. These mismatches are in the backbone regions only and do not affect any of the features.
Proper citation: RRID:Addgene_17454 Copy
Species: Other
Genetic Insert: mCherry with early endosome-targeting sequence
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pFX; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31735740
Proper citation: RRID:Addgene_174452 Copy
Species: Other
Genetic Insert: EGFP with early endosome-targeting sequence
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pFX; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31735740
Proper citation: RRID:Addgene_174454 Copy
Species: Homo sapiens
Genetic Insert: small heat shock protein27
Vector Backbone Description: Backbone Marker:BD Biosciences Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17597071
Proper citation: RRID:Addgene_17444 Copy
Genetic Insert: GFP
Vector Backbone Description: Backbone Size:8591; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19657394
Proper citation: RRID:Addgene_17446 Copy
Genetic Insert: GFP
Vector Backbone Description: Backbone Size:8068; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19657394
Proper citation: RRID:Addgene_17447 Copy
Genetic Insert: GFP
Vector Backbone Description: Backbone Size:5728; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:19657394
Proper citation: RRID:Addgene_17449 Copy
Species: Homo sapiens
Genetic Insert: SEPTIN2
Vector Backbone Description: Vector Backbone:pnEA-vH; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34237139
Proper citation: RRID:Addgene_174497 Copy
Species: Homo sapiens
Genetic Insert: VPS29
Vector Backbone Description: Backbone Size:4700; Vector Backbone:YFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18981234
Proper citation: RRID:Addgene_174520 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2595; Vector Backbone:pENTR1A; Vector Types:Mammalian Expression, Bacterial Expression, Yeast Expression, Insect Expression, Plant Expression, modified Gateway Entry vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18211686
Comments: Part of a modified Gateway Entry vector system for generating epitope tagged constructs. Clone your gene of interest into this vector using the BamHI and NotI cloning sites to obtain a N-terminal 6x MYC tagged Entry vector. The resulting Entry vector can be recombined into Gateway Destination vectors for bacteria, insect mammalian, plant or yeast expression via the Gateway LR reaction.
The tag is present in the Entry cassette to avoid the presence of att-derived protein linker sequences between the tag and gene or interest corresponding to the attB recombination site. A stop codon is already present within the vector (see plasmid map).
GenBank accession number: EU334818
Proper citation: RRID:Addgene_17457 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3023; Vector Backbone:pENTR1A; Vector Types:Mammalian Expression, Bacterial Expression, Yeast Expression, Insect Expression, Plant Expression, modified Gateway Entry vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18211686
Comments: Part of a modified Gateway Entry vector system for generating epitope tagged constructs. Clone your gene of interest into this vector using the BamHI and NotI cloning sites to obtain a C-terminal ECFP tagged Entry vector. The resulting Entry vector can be recombined into Gateway Destination vectors for bacteria, insect mammalian, plant or yeast expression via the Gateway LR reaction.
The tag is present in the Entry cassette to avoid the presence of att-derived protein linker sequences between the tag and gene or interest corresponding to the attB recombination site. A stop codon is already present within the vector (see plasmid map).
GenBank accession number: EU344826
Proper citation: RRID:Addgene_17465 Copy
Species: Homo sapiens
Genetic Insert: ubiquitination sgRNA
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene plasmid # 52961); Vector Backbone:lentiCRISPR v2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31692446
Proper citation: RRID:Addgene_174592 Copy
Genetic Insert: GM-CSF
Vector Backbone Description: Vector Backbone:mp71; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34396986
Proper citation: RRID:Addgene_174596 Copy
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