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Species: Synthetic
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:RSF1010; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29608282
Proper citation: RRID:Addgene_110604 Copy
Vector Backbone Description: Vector Backbone:RSF1010; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29608282
Proper citation: RRID:Addgene_110606 Copy
Species: Synthetic
Genetic Insert: mRFP dropout (RSF1010 origin)
Vector Backbone Description: Vector Backbone:RSF1010; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29608282
Proper citation: RRID:Addgene_110599 Copy
Vector Backbone Description: Backbone Size:2857; Vector Backbone:pSPL; Vector Types:Insect Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:17117155
Proper citation: RRID:Addgene_110743 Copy
Species: Arabidopsis thaliana
Genetic Insert: RPS5A promoter
Vector Backbone Description: Vector Backbone:pJY-RpABE; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29867128
Comments: Induces Y85H at FT
Proper citation: RRID:Addgene_112874 Copy
Species: Arabidopsis thaliana
Genetic Insert: RPS5A promoter
Vector Backbone Description: Vector Backbone:pKI1.1R; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29867128
Comments: A binary vector for plant adenine base editing through sgRNA cloning using AarI
Proper citation: RRID:Addgene_112872 Copy
Species: Arabidopsis thaliana
Genetic Insert: RPS5A promoter
Vector Backbone Description: Vector Backbone:pJY-RpABE; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29867128
Comments: Induces mis-splicing at PDS3
Proper citation: RRID:Addgene_112875 Copy
Vector Backbone Description: Backbone Marker:Lampropoulos et al; Backbone Size:4114; Vector Backbone:pZ001; Vector Types:Bacterial Expression, Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30083222
Comments: There are two additional BsaI cloning sites in the Cas9 sequences, but they should not affect the cloning process, since GoldenGate cloning can assemble four or more fragments into a backbone. The additional BsaI sites create four overhangs that are very different from the overhangs for integrating the sgRNAs, and there should be no major issues with fragment assembly or cloning efficiency.
Proper citation: RRID:Addgene_104439 Copy
Species: Other
Genetic Insert: AtCAS9 nickase D10A
Vector Backbone Description: Vector Backbone:pICH41308; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Comments: BsaI and BbsI sites have been removed from the sequence by site-directed mutagenesis, however the amino acid sequence is as in the original AtCas9 pDe-CAS9-D10A vector from H. Puchta lab.
Proper citation: RRID:Addgene_112071 Copy
Species: Other
Genetic Insert: AtDeadCAS9 D10A H840A
Vector Backbone Description: Vector Backbone:pICH41308; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Comments: BsaI and BbsI sites have been removed from the sequence by site-directed mutagenesis.
Proper citation: RRID:Addgene_112072 Copy
Species: Other
Genetic Insert: TagRFP
Vector Backbone Description: Vector Backbone:pICH41258; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Comments: BsaI and BbsI sites have been removed from the sequence by site-directed mutagenesis.
Proper citation: RRID:Addgene_112077 Copy
Species: Other
Genetic Insert: pcUBI promoter
Vector Backbone Description: Vector Backbone:pICH41295; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Comments: BsaI and BbsI sites have been removed from the sequence by site-directed mutagenesis.
Proper citation: RRID:Addgene_112075 Copy
Species: Other
Genetic Insert: AtCAS9 D10A without stop codon
Vector Backbone Description: Vector Backbone:pAGM1287; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Comments: BsaI and BbsI sites have been removed from the sequence by site-directed mutagenesis.
Proper citation: RRID:Addgene_112076 Copy
Species: Other
Genetic Insert: eCFP without stop codon
Vector Backbone Description: Vector Backbone:pAGM1287; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Comments: BsaI and BbsI sites have been removed from the sequence by site-directed mutagenesis.
Proper citation: RRID:Addgene_112082 Copy
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:pSC101; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30633862
Proper citation: RRID:Addgene_122136 Copy
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30525474
Proper citation: RRID:Addgene_122188 Copy
Species: Homo sapiens
Genetic Insert: SCAF8 isoform C CRISPR resistant
Vector Backbone Description: Backbone Size:2796; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31104839
Proper citation: RRID:Addgene_122466 Copy
Species: Other
Genetic Insert: T7 RNA polymerase
Vector Backbone Description: Vector Backbone:pBT21; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31332326
Comments: Please visit https://benchling.com/s/GBYnBWvd for additional information.
Proper citation: RRID:Addgene_122595 Copy
Vector Backbone Description: Backbone Marker:Jan-Willem Veening; Backbone Size:4314; Vector Backbone:pPEPZ-Plac; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31121970
Proper citation: RRID:Addgene_122635 Copy
Species: Bacillus subtilis
Genetic Insert: Sfp
Vector Backbone Description: Backbone Marker:Novagen/Merck Millipore; Backbone Size:3423; Vector Backbone:pCDF-1b; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30394635
Proper citation: RRID:Addgene_122851 Copy
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