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Species: Homo sapiens
Genetic Insert: ARHGAP30
Vector Backbone Description: Backbone Marker:Broad Institute; Vector Backbone:pLX307; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30777879
Proper citation: RRID:Addgene_117738 Copy
Species: Homo sapiens
Genetic Insert: ARHGAP30
Vector Backbone Description: Backbone Marker:Broad Institute; Vector Backbone:pLX311; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30777879
Proper citation: RRID:Addgene_117739 Copy
Species: Synthetic
Genetic Insert: hcRed1
Vector Backbone Description: Backbone Marker:Broad Institute; Backbone Size:9879; Vector Backbone:pLX311; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30777879
Proper citation: RRID:Addgene_117733 Copy
Species: Synthetic
Genetic Insert: mAID-nanobody
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Backbone Size:2539; Vector Backbone:pIRESpuro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30120238
Comments: codon-optimized A. thaliana AID (IAA17) is described in:
Baker, O. et al. RAC-tagging: Recombineering And Cas9-assisted targeting for protein tagging and conditional analyses. Sci Rep 6, 25529 (2016).
Proper citation: RRID:Addgene_117720 Copy
Species: Homo sapiens
Genetic Insert: MCL1L
Vector Backbone Description: Backbone Marker:Broad Institute; Vector Backbone:pLX307; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30777879
Proper citation: RRID:Addgene_117726 Copy
Species: Homo sapiens
Genetic Insert: MCL1L
Vector Backbone Description: Backbone Marker:Broad Institute; Vector Backbone:pLX311; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30777879
Proper citation: RRID:Addgene_117729 Copy
Species: Other
Genetic Insert: NanoLuc
Vector Backbone Description: Backbone Size:5907; Vector Backbone:pNBU2-ErmG; Vector Types:Bacterial Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28431252
Proper citation: RRID:Addgene_117728 Copy
Species: Homo sapiens
Genetic Insert: Human CRISPR Lentiviral Pooled Library against 496 kinase domains
Vector Backbone Description: Vector Backbone:LRG2.1; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29526696
Proper citation: RRID:Addgene_117725 Copy
Species: Bos taurus
Genetic Insert: BARKrgs peptide
Vector Backbone Description: Vector Backbone:pZac2.1; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34139149
Proper citation: RRID:Addgene_117691 Copy
Species: Mus musculus
Genetic Insert: Sod1
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_117710 Copy
Species: Oryza sativa
Genetic Insert: TRANSPORT INHIBITOR RESPONSE 1
Vector Backbone Description: Backbone Marker:RZPD; Backbone Size:4094; Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30120238
Comments: The codon-optimized NLS-TIR1 is described in:
Baker, O. et al. RAC-tagging: Recombineering And Cas9-assisted targeting for protein tagging and conditional analyses. Sci Rep 6, 25529 (2016).
Proper citation: RRID:Addgene_117716 Copy
Species: Synthetic
Genetic Insert: mAID-nanobody
Vector Backbone Description: Backbone Marker:RZPD; Backbone Size:4085; Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30120238
Comments: The codon-optimized A. thaliana AID (IAA17) is described in:
Baker, O. et al. RAC-tagging: Recombineering And Cas9-assisted targeting for protein tagging and conditional analyses. Sci Rep 6, 25529 (2016).
Proper citation: RRID:Addgene_117718 Copy
Species: Oryza sativa
Genetic Insert: TRANSPORT INHIBITOR RESPONSE 1
Vector Backbone Description: Backbone Marker:RZPD; Backbone Size:4089; Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30120238
Comments: The codon-optimized TIR1 is described in:
Baker, O. et al. RAC-tagging: Recombineering And Cas9-assisted targeting for protein tagging and conditional analyses. Sci Rep 6, 25529 (2016).
Proper citation: RRID:Addgene_117717 Copy
Species: Synthetic
Genetic Insert: mAID-nanobody
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Backbone Size:4850; Vector Backbone:pCDNA5 FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30120238
Comments: codon-optimized A. thaliana AID (IAA17) is described in:
Baker, O. et al. RAC-tagging: Recombineering And Cas9-assisted targeting for protein tagging and conditional analyses. Sci Rep 6, 25529 (2016).
Proper citation: RRID:Addgene_117713 Copy
Species: Homo sapiens
Genetic Insert: WIP
Vector Backbone Description: Backbone Marker:Liu et al (see Addgene's vector database for details); Backbone Size:6000; Vector Backbone:pMX-IRES-GFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16606694
Proper citation: RRID:Addgene_11772 Copy
Species: Mus musculus
Genetic Insert: Sod1
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_117701 Copy
Species: Mus musculus
Genetic Insert: Sod1
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_117702 Copy
Species: Mus musculus
Genetic Insert: Sod1
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_117709 Copy
Species: Mus musculus
Genetic Insert: Sod1
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_117708 Copy
Genetic Insert: CAG promoters, GFP, rtTR-KRAB-2SM2, Tet-off
Vector Backbone Description: Backbone Size:12859; Vector Backbone:None; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16432520
Comments: Transgenes can be expressed from any RNA Pol II promoter as part of
bicistronic unit comprising the KRAB-based repressor; tetO sequences
are inserted into the vector LTR. Tet-on and Tet-off versions rely on
repressors that bind in the absence or the presence of doxycycline,
respectively. Addition of Pol III promoter-small hairpin RNA cassette
allows for drug-controllable RNA interference (Tet-on shRNA).
Cloning shRNA into pLVET, pLVCT, and pLVPT vectors: first clone
shRNA into pLVTHM downstream of the tetO-H1 region. Then cut pLVTHM
containing your shRNA with MscI-FspI and clone the insert containing
the 3'LTR to target plasmid opened with MscI-FspI. FspI cuts into
AmpR. This means for inverted clones AmpR will not be restored; after
selection you will be left with clones with the shRNA cassette and
functional AmpR.
pLVTHM and packaging plasmid for this system are also available at Addgene http://www.addgene.org/rnaitools Please visit Trono lab website http://tronolab.epfl.ch to see frequently asked
questions on cloning strategies and packaging.
Proper citation: RRID:Addgene_11779 Copy
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