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Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Addgene #64247; Vector Backbone:pU6a:sgRNA#3; Vector Types:Mini-Golden; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:41630603
Proper citation: RRID:Addgene_200527 Copy
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Addgene #64247; Vector Backbone:pU6a:sgRNA#3; Vector Types:Mini-Golden; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:41630603
Proper citation: RRID:Addgene_200528 Copy
Genetic Insert: mCherry, NTRv2
Vector Backbone Description: Backbone Marker:Addgene #64247; Vector Backbone:pU6a:sgRNA#3; Vector Types:Mini-Golden; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:41630603
Proper citation: RRID:Addgene_200529 Copy
Species: Other
Genetic Insert: LoxP-EGFP-MALAT1-triplex
Vector Backbone Description: Vector Backbone:pX330; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40596718
Proper citation: RRID:Addgene_213048 Copy
Species: E. coli
Genetic Insert: leucyl-tRNA synthetase polyspecific mutant
Vector Backbone Description: Backbone Size:2302; Vector Backbone:pIDT-Smart; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:38904157
Proper citation: RRID:Addgene_217365 Copy
Genetic Insert: Split-SWITCHER1-TF1-dsRED
Vector Backbone Description: Vector Backbone:pX330; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40596718
Proper citation: RRID:Addgene_213045 Copy
Species: E. coli
Genetic Insert: E. coli leucine tRNA mutant, "LeuIGI1" for TAG suppression
Vector Backbone Description: Backbone Size:5498; Vector Backbone:pAAV-ITR-mCherry; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38904157
Proper citation: RRID:Addgene_217367 Copy
Species: E. coli
Genetic Insert: E. coli leucine tRNA mutant, "LeuIGI2" for TAG suppression
Vector Backbone Description: Backbone Size:5498; Vector Backbone:pAAV-ITR-mCherry; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38904157
Proper citation: RRID:Addgene_217368 Copy
Species: Homo sapiens
Genetic Insert: GSDMD
Vector Backbone Description: Backbone Size:5446; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39673710
Comments: Genscript's used their original construct OHu03821D (NM_024736.7) to clone our customized construct. Cloning method: CloneEZ;Vector: pcDNA3.1+/C-(K)DYK. The insert is a truncated construct coding for aa 1-275 of Gasdermin-D (GSDMD).
Proper citation: RRID:Addgene_218230 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:pHAGE2-TetO; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39919754
Proper citation: RRID:Addgene_216174 Copy
Species: SARS-CoV-2 virus
Genetic Insert: SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5400; Vector Backbone:pET21b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39673710
Comments: Silent mutation in the original sequence to delete a second NdeI site as NdeI was needed for cloning; codon coding for Q306 was mutated to codon coding for A306 to delete natural cleavage site of the 3CL-protease; two codons for glycine as a linker before the c-terminal 6x histag.
Proper citation: RRID:Addgene_224697 Copy
Species: Homo sapiens
Genetic Insert: MTOR
Vector Backbone Description: Backbone Size:8648; Vector Backbone:pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39524880
Comments: Used BbsI enzyme to clone in gRNA targeting gene of interest.
Proper citation: RRID:Addgene_208430 Copy
Species: Homo sapiens
Genetic Insert: SOX17
Vector Backbone Description: Vector Backbone:pHAGE2-EF1a; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39919754
Proper citation: RRID:Addgene_216200 Copy
Species: Homo sapiens
Genetic Insert: SOX17
Vector Backbone Description: Vector Backbone:pHAGE2-TetO; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39919754
Proper citation: RRID:Addgene_216181 Copy
Species: Homo sapiens
Genetic Insert: SOX17
Vector Backbone Description: Vector Backbone:pHAGE2-TetO; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39919754
Proper citation: RRID:Addgene_216182 Copy
Species: Homo sapiens
Genetic Insert: SOX17
Vector Backbone Description: Vector Backbone:pHAGE2-TetO; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39919754
Proper citation: RRID:Addgene_216185 Copy
Species: Homo sapiens
Genetic Insert: SOX17
Vector Backbone Description: Vector Backbone:pHAGE2-TetO; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39919754
Proper citation: RRID:Addgene_216184 Copy
Species: Homo sapiens
Genetic Insert: PKR
Vector Backbone Description: Backbone Size:8657; Vector Backbone:pKLV2-U6gRNA5(BbsI)-PGKpuro2AmCherry-W; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40135601
Comments: Used BbsI enzyme to clone in gRNA targeting gene of interest.
Proper citation: RRID:Addgene_208435 Copy
Species: Homo sapiens
Genetic Insert: EGFR
Vector Backbone Description: Backbone Size:8648; Vector Backbone:pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39524880
Comments: Used BbsI enzyme to clone in gRNA targeting gene of interest.
Proper citation: RRID:Addgene_208432 Copy
Species: Homo sapiens
Genetic Insert: ADAR1
Vector Backbone Description: Backbone Size:8648; Vector Backbone:pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40135601
Comments: Used BbsI enzyme to clone in gRNA targeting gene of interest.
Proper citation: RRID:Addgene_208433 Copy
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