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Species: Synthetic
Genetic Insert: EBFP, tTAn-InteinN
Vector Backbone Description: Backbone Size:5321; Vector Backbone:pAAV-EF1a-DIO-TVA950-T2A-CVS12G-WPRE; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35739125
Comments: Used Infusion cloning. 5' cloning site: XbaI (not destroyed), 3' cloning site: EcoRV (not destroyed). Please visit https://www.biorxiv.org/content/10.1101/2021.04.05.438407v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_172122 Copy
Species: Synthetic
Genetic Insert: InteinC-rtTAc
Vector Backbone Description: Backbone Size:5321; Vector Backbone:pAAV-EF1a-DIO-TVA950-T2A-CVS11G-WPRE; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35739125
Comments: Used Infusion cloning. 5' cloning site: XbaI (not destroyed), 3' cloning site: EcoRV (not destroyed). Please visit https://www.biorxiv.org/content/10.1101/2021.04.05.438407v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_172121 Copy
Species: Homo sapiens
Genetic Insert: EIF2B3
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33517449
Proper citation: RRID:Addgene_172087 Copy
Species: Homo sapiens
Genetic Insert: EIF2B3
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33517449
Proper citation: RRID:Addgene_172086 Copy
Species: Homo sapiens
Genetic Insert: EIF2B3
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33517449
Proper citation: RRID:Addgene_172085 Copy
Species: Homo sapiens
Genetic Insert: EIF2B3
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33517449
Proper citation: RRID:Addgene_172084 Copy
Species: Homo sapiens
Genetic Insert: beta catenin
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5400; Vector Backbone:pET-28 a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Description: This is an amino terminal HIS tag, TEV protease cleavage site, then human beta catenin domains R1C, subcloned in BamHI and Xho 1 sites.
Reference: Made by Ken-Ichi Takemaru.
Proper citation: RRID:Addgene_17200 Copy
Species: Xenopus laevis
Genetic Insert: beta catenin
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5400; Vector Backbone:pET-28 c; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Description: This is an amino terminal HIS tag, then beta catenin domains R10C, subcloned into BamH1 site.
Reference: Made by Ken-Ichi Takemaru.
Contains ARM domains 8-10 and C-terminal end of protein (starts with aa519)
Proper citation: RRID:Addgene_17202 Copy
Species: Danio rerio
Genetic Insert: Sp5l promoter
Vector Backbone Description: Backbone Marker:promega; Backbone Size:4818; Vector Backbone:pGL3-Basic; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Comments: Description: a fragment of putative zebrafish Sp5l promoter - 1203bp upstream of the 1st Exon.
(as defined by the 27i2 clone found in the microarray screen) - plus 51bp of 5'UTR from Exon1 clone.
upstream of firefly luciferase in the pGL3 Basic promoter. 6 of the putative Tcf/Lef sites mutated.
(all introduce additional restriction sites); cloned by PCR-based in vitro synthesis from 28 overlapping.
oligos. cloned NdeI / HindIII to exchange wild-type fragment in pGL3 Sp5l P1203.
Species of Sequence Origin: zebrafish.
Proper citation: RRID:Addgene_17209 Copy
Species: Homo sapiens
Genetic Insert: CbH-Cas9(H840A)-RT-T2A-Puro, hU6-pegRNA HEK3 CTT ins, hU6-sgRNA HEK3 +90
Vector Backbone Description: Vector Backbone:pCMV-PE2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34534334
Comments: Full sequence https://benchling.com/s/seq-LC7TExUM5DxOrjk2thkf.
Proper citation: RRID:Addgene_171996 Copy
Species: Homo sapiens
Genetic Insert: HEK3 CTT insertion pegRNA and CbH-Cas9-RT-T2A-Puro, hU6-pegRNA HEK3 CTT ins, hU6-sgRNA sham
Vector Backbone Description: Vector Backbone:pCMV-PE2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34534334
Comments: Full sequence https://benchling.com/s/seq-s7KGcyKNgXZDawwnudbC.
Proper citation: RRID:Addgene_171995 Copy
Species: Synthetic
Genetic Insert: CbH-Cas9-RT-T2A-GFP, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
Vector Backbone Description: Vector Backbone:pCMV-PE2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34534334
Comments: Full sequence https://benchling.com/s/seq-hFIfAYFnD7vtemxXRY19.
Proper citation: RRID:Addgene_171994 Copy
Species: Synthetic
Genetic Insert: CbH-Cas9-RT-T2A-Puro, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
Vector Backbone Description: Vector Backbone:pCMV-PE2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34534334
Comments: Full sequence https://benchling.com/s/seq-zQAEVlUC4JpjV6Gt4RFT.
Proper citation: RRID:Addgene_171992 Copy
Species: Synthetic
Genetic Insert: CbH-Cas9(H840A)-RT-T2A-Puro, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
Vector Backbone Description: Vector Backbone:pCMV-PE2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34534334
Comments: Full sequence: https://benchling.com/s/seq-btUItL879sEOzAzXhUuy.
Proper citation: RRID:Addgene_171991 Copy
Species: Homo sapiens
Genetic Insert: CD63
Vector Backbone Description: Backbone Size:9331; Vector Backbone:pLenti-CMV Blast DEST (706-1); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32350252
Proper citation: RRID:Addgene_172117 Copy
Species: Homo sapiens
Genetic Insert: hNGN2
Vector Backbone Description: Backbone Size:13245; Vector Backbone:pUCM; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36459969
Comments: This plasmid has been found to run as a multimer. Plasmid multimerization often does not impact plasmid function, but may reduce transformation efficiencies. You may need to screen multiple colonies to isolate the monomeric version of this plasmid. If you still have trouble isolating the monomeric version, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid.
Proper citation: RRID:Addgene_172115 Copy
Species: N.Neisseria meningitidis serogroup B (strain MC58)
Genetic Insert: MetN
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5245; Vector Backbone:pET21b+; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34409939
Proper citation: RRID:Addgene_172114 Copy
Species: Homo sapiens
Genetic Insert: Chibby
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4900; Vector Backbone:pGEX-4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12712206
Comments: Description: The N terminal half (amino acids 1-63) of human Chibby was amplified by PCR and subcloned into EcoR1 and Xho1 sites of pGEX-4T-1. A TEV protease site was included in the 5' primer.
Reference: K. Takemaru et al., Nature 422: 905 (2003)
Proper citation: RRID:Addgene_17196 Copy
Species: Homo sapiens
Genetic Insert: Chibby
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4900; Vector Backbone:pGEX-4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12712206
Comments: Description: The C terminal half (amino acids 64-126) of human Chibby was amplified by PCR and subcloned into EcoR1 and Xho1 sites of pGEX-4T-1. A TEV protease site was included in the 5' primer. Insert contains 3'UTR of Cby.
Reference: K. Takemaru et al., Nature 422: 905 (2003)
Proper citation: RRID:Addgene_17197 Copy
Species: Homo sapiens
Genetic Insert: beta catenin
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5400; Vector Backbone:pET-28 a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Description: This is full length human beta catenin cDNA with a TEV protease site at the amino terminus following the HIS tag. Cloned into BamH1 and Xho1 sites.
Reference: Made by Ken-Ichi Takemaru.
Proper citation: RRID:Addgene_17198 Copy
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