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Genetic Insert: Enhanced green fluorescent protein shRNA #2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2556; Vector Backbone:pENTR1A; Vector Types:RNAi, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19657394
Comments: shRNA sequence is- 5'-CCGCTGGAGTACAACTACAACTTCAAGAGAGTTGTAGTTGTACTCCAGCTTTTT
Proper citation: RRID:Addgene_17471 Copy
Genetic Insert: Firefly Luciferase
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2240; Vector Backbone:pENTR4; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19657394
Proper citation: RRID:Addgene_17473 Copy
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:8931; Vector Backbone:pQCXIP; Vector Types:Mammalian Expression, Retroviral, RNAi, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:19657394
Proper citation: RRID:Addgene_17474 Copy
Species: N. benthamiana
Genetic Insert: GG_NbZAR1syn-L17E-4xMyc, Kmr plant selection marker
Vector Backbone Description: Backbone Marker:https://www.addgene.org/48076/; Vector Backbone:pICH86988; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34633454
Proper citation: RRID:Addgene_174772 Copy
Species: N. benthamiana
Genetic Insert: GG_NbZAR1syn-L17E/D481V-4xMyc, Kmr plant selection marker
Vector Backbone Description: Backbone Marker:https://www.addgene.org/48076/; Vector Backbone:pICH86988; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34633454
Proper citation: RRID:Addgene_174773 Copy
Species: N. benthamiana
Genetic Insert: GG_NbZAR1syn-6xHA, Kmr plant selection marker
Vector Backbone Description: Backbone Marker:https://www.addgene.org/48076/; Vector Backbone:pICH86988; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34633454
Proper citation: RRID:Addgene_174774 Copy
Species: N. benthamiana
Genetic Insert: GG_NbZAR1syn-D481V-6xHA, Kmr plant selection marker
Vector Backbone Description: Backbone Marker:https://www.addgene.org/48076/; Vector Backbone:pICH86988; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34633454
Proper citation: RRID:Addgene_174775 Copy
Genetic Insert: Enhanced green fluorescent protein shRNA #1
Vector Backbone Description: Backbone Size:7919; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19657394
Comments: shRNA oligo sequence - CGAAGCAGCACGACTTCTTCTTCAAGAGAGAAGAAGTCGTGCTGCTTCTTTTT
Proper citation: RRID:Addgene_17479 Copy
Species: N. benthamiana
Genetic Insert: GG_NbZAR1syn without STOP codon
Vector Backbone Description: Backbone Marker:https://www.addgene.org/47996/; Vector Backbone:pICSL01005; Vector Types:Unspecified; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34633454
Proper citation: RRID:Addgene_174766 Copy
Species: Homo sapiens
Genetic Insert: PARP1-G972R
Vector Backbone Description: Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34465625
Comments: Protein residues 1-1014 (V762A, G972R) (see PMID: 9315851 for detail about the mutation)
Proper citation: RRID:Addgene_174800 Copy
Species: N. benthamiana
Genetic Insert: GG_NbZAR1syn-L17E without STOP codon
Vector Backbone Description: Backbone Marker:https://www.addgene.org/47996/; Vector Backbone:pICSL01005; Vector Types:Unspecified; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34633454
Proper citation: RRID:Addgene_174768 Copy
Species: Homo sapiens
Genetic Insert: PARP1-Y986H
Vector Backbone Description: Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34465625
Comments: Protein residues 1-1014 (V762A, Y986H) (see PMID: 9315851 for detail about the mutation)
Proper citation: RRID:Addgene_174801 Copy
Species: Homo sapiens
Genetic Insert: PARP1-Y986S
Vector Backbone Description: Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34465625
Comments: Protein residues 1-1014 (V762A, Y986S) (see PMID: 9315851 for detail about the mutation)
Proper citation: RRID:Addgene_174802 Copy
Species: Homo sapiens
Genetic Insert: PARP1deltaART
Vector Backbone Description: Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34465625
Comments: Protein residues 1-784 (V762A)
Proper citation: RRID:Addgene_174804 Copy
Vector Backbone Description: Backbone Size:8007; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19657394
Proper citation: RRID:Addgene_17482 Copy
Species: N. benthamiana
Genetic Insert: GG_synthesized NbZAR1 (NbZAR1syn) with STOP codon
Vector Backbone Description: Backbone Marker:https://www.addgene.org/47998/; Vector Backbone:pICH41308; Vector Types:Unspecified; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34633454
Proper citation: RRID:Addgene_174762 Copy
Species: N. benthamiana
Genetic Insert: GG_NbZAR1syn-D481V with STOP codon
Vector Backbone Description: Backbone Marker:https://www.addgene.org/47998/; Vector Backbone:pICH41308; Vector Types:Unspecified; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34633454
Proper citation: RRID:Addgene_174763 Copy
Species: M. smegmatis
Genetic Insert: MSMEG_0948
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin
Defining Citation: PMID:33653882
Comments: 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed)
Proper citation: RRID:Addgene_174952 Copy
Species: M. smegmatis
Genetic Insert: MSMEG_0952
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin
Defining Citation: PMID:33653882
Comments: 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed)
Proper citation: RRID:Addgene_174954 Copy
Species: M. smegmatis
Genetic Insert: MSMEG_0953
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin
Defining Citation: PMID:33653882
Comments: 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed)
Proper citation: RRID:Addgene_174955 Copy
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