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Species: Synthetic
Genetic Insert: Para sfGFP.2TAG; Plpp pylS; PproK pylT; AraC
Vector Backbone Description: Vector Backbone:p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34168131
Proper citation: RRID:Addgene_172153 Copy
Species: Synthetic
Genetic Insert: Ppsp gIII.1TAG, luxAB; Plpp 32A; PproK pylT
Vector Backbone Description: Vector Backbone:ColE1; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34168131
Proper citation: RRID:Addgene_172150 Copy
Species: Synthetic
Genetic Insert: pCDF.B.(SD4)CD
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34168131
Proper citation: RRID:Addgene_172146 Copy
Species: Gibbon Ape Leukemia Virus
Genetic Insert: Envelope protein of Gibbon Ape Leukemia Virus (codon-optimized version)
Vector Backbone Description: Backbone Size:4816; Vector Backbone:phCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34452336
Comments: Please note that significantly lower amounts of this codon optimized plasmid are required for efficient packaging. Depending on the vector type and protocol used so far, optimal titers are achieved with 8 to 30 times less plasmid compared to non-codon optimized GALV env. We suggest to start with 0.02 µg per 1x10^6 293T packaging cells (resulting in about 0.1 µg phCMV-coGALVenv-C4070A plasmid per 10 cm cell culture dish). Full details are given in the publication.
Proper citation: RRID:Addgene_172217 Copy
Species: Synthetic
Genetic Insert: catalytic mutant of Cas9 endonuclease from the Campylobacter jejuni Type II CRISPR/Cas system
Vector Backbone Description: Backbone Size:5353; Vector Backbone:His6 TEV LIC cloning vector (1B); Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32596446
Proper citation: RRID:Addgene_172216 Copy
Species: Synthetic
Genetic Insert: Cas9 endonuclease from the Campylobacter jejuni Type II CRISPR/Cas system
Vector Backbone Description: Backbone Size:5353; Vector Backbone:His6 TEV LIC cloning vector (1B); Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32596446
Proper citation: RRID:Addgene_172215 Copy
Vector Backbone Description: Backbone Size:2074; Vector Backbone:pIVTR; Vector Types:Other; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_172292 Copy
Species: Homo sapiens
Genetic Insert: PuroR-F2A-mClover3
Vector Backbone Description: Backbone Size:2074; Vector Backbone:pIVTR; Vector Types:Other; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_172291 Copy
Species: Homo sapiens
Genetic Insert: PuroR
Vector Backbone Description: Backbone Size:2074; Vector Backbone:pIVTR; Vector Types:Other; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_172290 Copy
Vector Backbone Description: Backbone Marker:Koropatkin, N.M. (PMID: 18611383); Backbone Size:4172; Vector Backbone:pExchange-tdk; Vector Types:Genetic insertion in Prevotella copri; Bacterial Resistance:Amp + DAP
Defining Citation: PMID:34676563
Comments: Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_172212 Copy
Species: Synthetic
Genetic Insert: PT7/Lac-[His6-TEV-SUMO-PylB.JM10.1deopt]
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34168131
Proper citation: RRID:Addgene_172177 Copy
Vector Backbone Description: Backbone Marker:Koropatkin, N.M. (PMID: 18611383); Backbone Size:4172; Vector Backbone:pExchange-tdk; Vector Types:Genetic insertion in Prevotella copri; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34676563
Proper citation: RRID:Addgene_172210 Copy
Species: Synthetic
Genetic Insert: PT7/Lac-[His6-TEV-SUMO-PylB.WTopt]
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34168131
Proper citation: RRID:Addgene_172175 Copy
Species: Synthetic
Genetic Insert: PT7/Lac-[His6-TEV-SUMO-PylB.JM10.1]
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34168131
Proper citation: RRID:Addgene_172174 Copy
Species: Homo sapiens
Genetic Insert: mTagBFP
Vector Backbone Description: Backbone Size:2074; Vector Backbone:pIVTR; Vector Types:Other; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_172295 Copy
Species: Mus musculus
Genetic Insert: KIF1A
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4731; Vector Backbone:eGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27851960
Proper citation: RRID:Addgene_172207 Copy
Species: Mus musculus
Genetic Insert: KIF5B
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4731; Vector Backbone:eGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27851960
Proper citation: RRID:Addgene_172204 Copy
Species: Synthetic
Genetic Insert: Pem7 luxCDA(B.1TAG)E; Plpp pylS; PproK pylT
Vector Backbone Description: Vector Backbone:p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34168131
Proper citation: RRID:Addgene_172169 Copy
Species: Homo sapiens
Genetic Insert: EGFP-N1-1x
Vector Backbone Description: Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28484218
Proper citation: RRID:Addgene_172281 Copy
Species: Mus musculus
Genetic Insert: KIF5A
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4728; Vector Backbone:eGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26527003
Proper citation: RRID:Addgene_172201 Copy
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