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Genetic Insert: lacZ
Vector Backbone Description: Vector Backbone:pDGB3_Omega1_domesticated; Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32163700
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.02.29.971002v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_136069 Copy
Species: Synthetic
Genetic Insert: Even spacer
Vector Backbone Description: Vector Backbone:pCsA; Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32163700
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.02.29.971002v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_136075 Copy
Species: Marchantia polymorpha
Genetic Insert: p5-35S:HygR p5-MpUBE2:eGFP-Lti6b
Vector Backbone Description: Vector Backbone:pCsA; Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32163700
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.02.29.971002v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_136134 Copy
Vector Backbone Description: Vector Backbone:pYPQ141-ZmUbi-RZ-Lb; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32170285
Comments: sgRNA scaffold is from: Teng, F., Cui, T., Feng, G. et al. Repurposing CRISPR-Cas12b for mammalian genome engineering. Cell Discov 4, 63 (2018) doi:10.1038/s41421-018-0069-3
Proper citation: RRID:Addgene_136372 Copy
Vector Backbone Description: Vector Backbone:pYPQ141-ZmUbi-RZ-Lb; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32170285
Comments: sgRNA scaffold is from: Teng, F., Cui, T., Feng, G. et al. Repurposing CRISPR-Cas12b for mammalian genome engineering. Cell Discov 4, 63 (2018) doi:10.1038/s41421-018-0069-4
Proper citation: RRID:Addgene_136373 Copy
Vector Backbone Description: Vector Backbone:pYPQ141-ZmUbi-RZ-Lb; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32170285
Comments: sgRNA scaffold and MS2 sequence are from: Teng, F., Cui, T., Feng, G. et al. Repurposing CRISPR-Cas12b for mammalian genome engineering. Cell Discov 4, 63 (2018) doi:10.1038/s41421-018-0069-5
Proper citation: RRID:Addgene_136374 Copy
Vector Backbone Description: Vector Backbone:pYPQ141-ZmUbi-RZ-Lb; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32170285
Proper citation: RRID:Addgene_136377 Copy
Species: Francisella novicida U112
Genetic Insert: dFnCas12a-TV
Vector Backbone Description: Vector Backbone:pYPQ167; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32170285
Comments: TV activation system is from: Li, Z., Zhang, D., Xiong, X. et al. A potent Cas9-derived gene activator for plant and mammalian cells. Nature Plants 3, 930–936 (2017) doi:10.1038/s41477-017-0046-0
Proper citation: RRID:Addgene_136379 Copy
Species: Alicyclobacillus acidiphilus
Genetic Insert: AaCas12b (D570A)-TV-T2A-MCP-TV
Vector Backbone Description: Vector Backbone:pYPQ167; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32170285
Comments: TV activation system is from: Li, Z., Zhang, D., Xiong, X. et al. A potent Cas9-derived gene activator for plant and mammalian cells. Nature Plants 3, 930–936 (2017) doi:10.1038/s41477-017-0046-2
Proper citation: RRID:Addgene_136381 Copy
Species: Alicyclobacillus acidiphilus
Genetic Insert: AaCas12b (D570A)-TV-T2A-MCP-VPR
Vector Backbone Description: Vector Backbone:pYPQ167; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32170285
Comments: TV activation system is from: Li, Z., Zhang, D., Xiong, X. et al. A potent Cas9-derived gene activator for plant and mammalian cells. Nature Plants 3, 930–936 (2017) doi:10.1038/s41477-017-0046-3; VPR activation system is from: Chavez A, Scheiman J, Vora S, Pruitt BW, Tuttle M, E PRI, et al. Highly efficient Cas9-mediated transcriptional programming. Nat Methods. 2015;12(4):326–8. 10.1038/nmeth.3312
Proper citation: RRID:Addgene_136382 Copy
Vector Backbone Description: Backbone Size:7912; Vector Backbone:N/A; Vector Types:E. coli-S. arueus shuttle vector; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:9045821
Proper citation: RRID:Addgene_13656 Copy
Species: Synthetic
Genetic Insert: R-TAGA-Evo1 qtRNA
Vector Backbone Description: Vector Backbone:RK2 SpecR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34588441
Comments: The lacI repressor must also be expressed in order to make the ProK-LacI promoter IPTG-inducible. These can be provided by the host strain, or on another plasmid. For example, E coli S2060s (Addgene Bacterial strain #105064), encode lacI.
Proper citation: RRID:Addgene_134798 Copy
Species: Synthetic
Genetic Insert: Wildtype S tRNA (decodes TCG)
Vector Backbone Description: Vector Backbone:RK2 SpecR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34588441
Comments: The lacI repressor must also be expressed in order to make the ProK-LacI promoter IPTG-inducible. These can be provided by the host strain, or on another plasmid. For example, E coli S2060s (Addgene Bacterial strain #105064), encode lacI.
Proper citation: RRID:Addgene_134803 Copy
Species: Synthetic
Genetic Insert: Wildtype S tRNA (decodes TCG)
Vector Backbone Description: Vector Backbone:RK2 SpecR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34588441
Comments: The lacI repressor must also be expressed in order to make the ProK-LacI promoter IPTG-inducible. These can be provided by the host strain, or on another plasmid. For example, E coli S2060s (Addgene Bacterial strain #105064), encode lacI.
Proper citation: RRID:Addgene_134804 Copy
Species: Synthetic
Genetic Insert: W-TAGA qtRNA
Vector Backbone Description: Vector Backbone:RK2 SpecR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34588441
Comments: The lacI repressor must also be expressed in order to make the ProK-LacI promoter IPTG-inducible. These can be provided by the host strain, or on another plasmid. For example, E coli S2060s (Addgene Bacterial strain #105064), encode lacI.
Proper citation: RRID:Addgene_134805 Copy
Species: Synthetic
Genetic Insert: Y-TAGA-Evo1 qtRNA
Vector Backbone Description: Vector Backbone:RK2 SpecR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34588441
Comments: The lacI repressor must also be expressed in order to make the ProK-LacI promoter IPTG-inducible. These can be provided by the host strain, or on another plasmid. For example, E coli S2060s (Addgene Bacterial strain #105064), encode lacI.
Proper citation: RRID:Addgene_134808 Copy
Vector Backbone Description: Backbone Size:6742; Vector Backbone:pPZP200; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Comments: Contains the 564bp bialaphos resistance (bar) gene from Streptomyces hygroscopicus
Proper citation: RRID:Addgene_134770 Copy
Vector Backbone Description: Backbone Size:6742; Vector Backbone:pPZP200; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:16249869
Comments: Contains the 564bp bialaphos resistance (bar) gene from Streptomyces hygroscopicus
Proper citation: RRID:Addgene_134771 Copy
Species: Synthetic
Genetic Insert: ilvA-acp
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pH9; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31395784
Proper citation: RRID:Addgene_135163 Copy
Species: Synthetic
Genetic Insert: CysJ-infA overlapping region in CI-2
Vector Backbone Description: Backbone Size:3100; Vector Backbone:pH9; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31395784
Proper citation: RRID:Addgene_135165 Copy
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