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Species: Schmidtea mediterranea
Genetic Insert: follistatin in situ probe
Vector Backbone Description: Backbone Marker:Phillip Newmark (Addgene plasmid # 26536); Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23297191
Comments: Cloning primers: F-TATGTCCGGTCGGAGAGTTT, R-GAGGTGGGGCATTTGATACA
Proper citation: RRID:Addgene_99066 Copy
Species: Schmidtea mediterranea
Genetic Insert: LDLRR-2 in situ probe
Vector Backbone Description: Backbone Marker:Phillip Newmark (Addgene plasmid # 26536); Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:27612384
Comments: Cloning primers: F-GCCCAGACAAAGAAGATGGA, R-TGTTGCACAGCATTCGTTTT
Note: the LDLRR-2 insert has a 15 base pair deletion compared to JX010572.1. The depositor has confirmed this does not affect plasmid function.
Proper citation: RRID:Addgene_99094 Copy
Species: Schmidtea mediterranea
Genetic Insert: LDLRR-3 in situ probe
Vector Backbone Description: Backbone Marker:Phillip Newmark (Addgene plasmid # 26536); Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:27612384
Comments: Cloning primers: F-GATGTTTAGACCATAGTATATG, R-GAATTGAAGATAAAATAAATTG
Proper citation: RRID:Addgene_99095 Copy
Species: Schmidtea mediterranea
Genetic Insert: Arrowhead in situ probe
Vector Backbone Description: Backbone Marker:Phillip Newmark (Addgene plasmid # 26536); Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:27612384
Comments: Cloning primers: F-TTGATGGAAAGTGTTTTGGTTG, R-TTTTCAGTTTTCCCGTTACAAA
Proper citation: RRID:Addgene_99097 Copy
Species: Schmidtea mediterranea
Genetic Insert: FLI-1 in situ probe
Vector Backbone Description: Backbone Marker:Phillip Newmark (Addgene plasmid # 26536); Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:27612384
Comments: Cloning primers: F-CGGACTTGGACTATCGGAAA, R-CCACAGTACCGGCATGATTA
Proper citation: RRID:Addgene_99099 Copy
Species: Schmidtea mediterranea
Genetic Insert: IF-1 in situ probe
Vector Backbone Description: Backbone Marker:Phillip Newmark (Addgene plasmid # 26536); Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:27612384
Comments: Cloning primers: F-AATGAATTGTCCGCTCTTGC, R-TGAATTATTGAATCGGCTTCTG
Proper citation: RRID:Addgene_99100 Copy
Species: Schmidtea mediterranea
Genetic Insert: GS-1 in situ probe
Vector Backbone Description: Backbone Marker:Phillip Newmark (Addgene plasmid # 26536); Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:27612384
Comments: Cloning primers: F-ATCATCCATGGTTTGGCATT, R-CGTCCGGTTAAACGATGTCT
Proper citation: RRID:Addgene_99101 Copy
Species: Schmidtea mediterranea
Genetic Insert: jagged-like in situ probe
Vector Backbone Description: Backbone Marker:Phillip Newmark (Addgene plasmid # 26536); Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:27612384
Comments: Cloning primers: F-AAAAACCGATAGTCGAAAGC, R-ATCGTCAACTTTTTCGGAAT
Proper citation: RRID:Addgene_99103 Copy
Species: Synthetic
Genetic Insert: tdTomato
Vector Backbone Description: Backbone Marker:Hyung-song Nam, Capecchi laboratory; Vector Backbone:Custom; Vector Types:Mammalian Expression, Bacterial Expression, Mouse Targeting, Cre/Lox; Bacterial Resistance:Ampicillin and Kanamycin
Comments: A reporter expression cassette with short homology arms to Hipp11 on either side. Constructed originally for generating Hipp11 reporter allele targeting vector by recombineering. Untested for other purposes, but may be useful for CRISPR knock-in as well.
Proper citation: RRID:Addgene_180156 Copy
Species: Homo sapiens
Genetic Insert: Homo sapiens hemoglobin subunit beta (HBB), Normal cDNA fragment (Exon1/2)
Vector Backbone Description: Backbone Marker:Invitrogen (Life Technologies); Backbone Size:3930; Vector Backbone:pCR2.1-TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:32933098
Proper citation: RRID:Addgene_87847 Copy
Genetic Insert: pMarC9-R6k
Vector Backbone Description: Backbone Marker:Victor de Lorenzo lab; Vector Backbone:pBAM1; Vector Types:; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:30962569
Comments: BW29427 is an E. coli strain with diaminopimelic acid (DAP) auxotrophy. BW29427 growth requires 300uM DAP even when cultured in LB. Importantly, BW29427 does not grow in the absence of DAP, which simplifies counterselection of this host strain following biparental mating with Vibrio natriegens.
Please visit https://www.biorxiv.org/content/early/2016/06/12/058487 for bioRxiv preprint.
Proper citation: RRID:Addgene_89477 Copy
Species: SV40, Entacmaea quadricolor
Genetic Insert: nuclear localization signal (nls) turboRFP
Vector Backbone Description: Backbone Size:9530; Vector Backbone:pK038.CAG-loxP-stop-loxP-EGFP-ires-tTA-WPRE (Supernova); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:27775045
Comments: Backbone plasmid pK038.CAG-loxP-stop-loxP-EGFP-ires-tTA-WPRE (Supernova) also submitted in addgene database.
Proper citation: RRID:Addgene_89587 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3956; Vector Backbone:PCR4-TOPO; Vector Types:Mammalian Expression, Mouse Targeting, CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:39762235
Proper citation: RRID:Addgene_211940 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3956; Vector Backbone:PCR4-TOPO; Vector Types:Mammalian Expression, Mouse Targeting, CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:39762235
Proper citation: RRID:Addgene_211942 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:13909; Vector Backbone:PCR4-TOPO; Vector Types:Mammalian Expression, Mouse Targeting, CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:39762235
Proper citation: RRID:Addgene_216394 Copy
Species: Other
Genetic Insert: BsamAIM and BsaIR
Vector Backbone Description: Vector Backbone:Modified pCR2.1 TOPO (pBR BsmAIM Kan) and p15A origin plasmid (p15A BsaIR Amp); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:34061896
Comments: Please take both plasmid sequences into account when performing sequencing and digest assays.
Proper citation: RRID:Addgene_206208 Copy
Species: Mus musculus
Genetic Insert: Fzd6
Vector Backbone Description: Vector Backbone:pCR2.1; Vector Types:cloning vector; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:37622728
Proper citation: RRID:Addgene_208365 Copy
Species: Synthetic
Genetic Insert: E2-Crimson cassette flanked with homology arms for recombination into the S. alvi wkB2 genome to replace clpA (SALWKB2_RS04465)
Vector Backbone Description: Backbone Size:1887; Vector Backbone:pYTK095; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:37786689
Comments: Please visit https://doi.org/10.1101/2023.09.19.558440 for bioRxiv preprint.
Proper citation: RRID:Addgene_209550 Copy
Species: Synthetic
Genetic Insert: PAGFP-FLAG-Kan
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Proper citation: RRID:Addgene_211834 Copy
Species: Synthetic
Genetic Insert: sfGFP-FLAG-Kan
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Proper citation: RRID:Addgene_211833 Copy
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