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Species: Homo sapiens
Genetic Insert: TFDP2
Vector Backbone Description: Backbone Size:2693; Vector Backbone:pDonor-255; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Proper citation: RRID:Addgene_70630 Copy
Species: Homo sapiens
Genetic Insert: TIAM2
Vector Backbone Description: Backbone Size:2693; Vector Backbone:pDonor-255; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Comments: c1449t silent mutation not noted in Refseq
Proper citation: RRID:Addgene_70633 Copy
Species: Homo sapiens
Genetic Insert: TP53
Vector Backbone Description: Backbone Size:2693; Vector Backbone:pDonor-255; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Proper citation: RRID:Addgene_70635 Copy
Species: Homo sapiens
Genetic Insert: TP53
Vector Backbone Description: Backbone Size:2693; Vector Backbone:pDonor-255; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Proper citation: RRID:Addgene_70636 Copy
Species: Homo sapiens
Genetic Insert: TSC2
Vector Backbone Description: Backbone Size:2693; Vector Backbone:pDonor-255; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Proper citation: RRID:Addgene_70639 Copy
Species: Homo sapiens
Genetic Insert: STK3
Vector Backbone Description: Backbone Size:2693; Vector Backbone:pDonor-255; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Proper citation: RRID:Addgene_70621 Copy
Species: Homo sapiens
Genetic Insert: STK4
Vector Backbone Description: Backbone Size:2693; Vector Backbone:pDonor-255; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Proper citation: RRID:Addgene_70624 Copy
Species: Homo sapiens
Genetic Insert: STK4
Vector Backbone Description: Backbone Size:2693; Vector Backbone:pDonor-255; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Proper citation: RRID:Addgene_70623 Copy
Species: Homo sapiens
Genetic Insert: TBK1
Vector Backbone Description: Backbone Size:2693; Vector Backbone:pDonor-255; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Comments: t978a silent mutation not noted in Refseq
Proper citation: RRID:Addgene_70625 Copy
Species: Homo sapiens
Genetic Insert: TFDP1
Vector Backbone Description: Backbone Size:2693; Vector Backbone:pDonor-255; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Proper citation: RRID:Addgene_70628 Copy
Species: Homo sapiens
Genetic Insert: TFDP2
Vector Backbone Description: Backbone Size:2693; Vector Backbone:pDonor-255; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Proper citation: RRID:Addgene_70629 Copy
Species: Homo sapiens
Genetic Insert: VAV1
Vector Backbone Description: Backbone Size:2693; Vector Backbone:pDonor-255; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Proper citation: RRID:Addgene_70642 Copy
Species: Homo sapiens
Genetic Insert: YAP1
Vector Backbone Description: Backbone Size:2693; Vector Backbone:pDonor-255; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Proper citation: RRID:Addgene_70644 Copy
Species: Homo sapiens
Genetic Insert: YAP1
Vector Backbone Description: Backbone Size:2693; Vector Backbone:pDonor-255; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Proper citation: RRID:Addgene_70643 Copy
Species: bacteria
Genetic Insert: bar and GUS cassettes
Vector Backbone Description: Backbone Size:7132; Vector Backbone:pPZP201; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:18256901
Comments: Discrepancies between Depositor's full sequence and Addgene's QC sequence are not of functional concern.
Proper citation: RRID:Addgene_73934 Copy
Species: Synthetic
Genetic Insert: bar
Vector Backbone Description: Backbone Size:7132; Vector Backbone:pPZP201; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:15034747
Comments: This vector is suitable for plant transformation and has been used as standard service empty vector control.
Proper citation: RRID:Addgene_73933 Copy
Species: Synthetic
Genetic Insert: bar
Vector Backbone Description: Backbone Size:7132; Vector Backbone:pPZP201; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:15034747
Comments: This vector is suitable hpRNA-mediated gene silencing in tissue (seed) specific manner.
Please see the Plant Transformation Core Facility website for more information on using this plasmid: http://plantsci.missouri.edu/muptcf/pzy101.html
The vector has following features:
1. It has two replicate origins that allow to replicate in both E. coli and Agrobacterium tumefaciens cells (at higher copy numbers).
2. It also has bom site that allows you to use tri-parental mating to mobilize the vector to Agrobacterium cells.
3. The aadA locus confers Spectinomycin (100 mg/L) or streptomycin (100 mg/L) resistance and thus enable to use either or both of these two antibiotics for the vector selection.
4. The multiple cloning site (MCS) adjacent to the double CaMV35S promoter will allow you to insert your cassette. This CaMV35S promoter is used to drive the bar ORF and, therefore, is not used in front to drive your gene of interest. You need to assemble your own expression cassette including promoter, gene coding sequence, and terminator, and then insert to anywhere between Hind III and EcoR I within the MCS.
Special notes:
1. Insert your gene expression cassette in such a way that your promoter is adjacent to the promoter driving the bar gene selectable marker. This is to prevent transcription read-through from your gene expression cassette.
2. In order to use the EcoR I site within the MCS, a minimal ratio of this enzyme versus DNA is recommended to avoid star activity. When HindIII and EcoRI are in combined use, even less of each enzyme is required, and reaction should be conducted in a large volume to avoid excessive enzymatic activity.
Proper citation: RRID:Addgene_73932 Copy
Species: Synthetic
Genetic Insert: sgRNA pyk (NT)
Vector Backbone Description: Vector Backbone:pAL374; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:26829286
Proper citation: RRID:Addgene_74072 Copy
Species: Synthetic
Genetic Insert: sgRNA pyk (T)
Vector Backbone Description: Vector Backbone:pAL374; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:26829286
Proper citation: RRID:Addgene_74070 Copy
Species: Synthetic
Genetic Insert: sgRNA rfp (T)
Vector Backbone Description: Vector Backbone:pAL374; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:26829286
Proper citation: RRID:Addgene_74075 Copy
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