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pCsC
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_136069 lacZ Spectinomycin PMID:32163700 Please visit https://www.biorxiv.org/content/10.1101/2020.02.29.971002v1 for bioRxiv preprint. Vector Backbone:pDGB3_Omega1_domesticated; Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin 2026-08-15 01:06:01 1
pCsA_spacer
 
Resource Report
Resource Website 		RRID:Addgene_136075 Even spacer Synthetic Spectinomycin PMID:32163700 Please visit https://www.biorxiv.org/content/10.1101/2020.02.29.971002v1 for bioRxiv preprint. Vector Backbone:pCsA; Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin BsaI/ SapI domesticated 2026-08-15 01:06:01 0
L2_UBE2:eG-Lt-CsA
 
Resource Report
Resource Website 		RRID:Addgene_136134 p5-35S:HygR p5-MpUBE2:eGFP-Lti6b Marchantia polymorpha Spectinomycin PMID:32163700 Please visit https://www.biorxiv.org/content/10.1101/2020.02.29.971002v1 for bioRxiv preprint. Vector Backbone:pCsA; Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin BsaI/ SapI domesticated 2026-08-15 01:06:02 0
pYPQ141-ZmUbi-RZ-Aa1.2.3
 
Resource Report
Resource Website 		RRID:Addgene_136372 Spectinomycin PMID:32170285 sgRNA scaffold is from: Teng, F., Cui, T., Feng, G. et al. Repurposing CRISPR-Cas12b for mammalian genome engineering. Cell Discov 4, 63 (2018) doi:10.1038/s41421-018-0069-3 Vector Backbone:pYPQ141-ZmUbi-RZ-Lb; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin 2026-08-15 01:06:06 0
pYPQ141-ZmUbi-RZ-Aa1.2
 
Resource Report
Resource Website 		RRID:Addgene_136373 Spectinomycin PMID:32170285 sgRNA scaffold is from: Teng, F., Cui, T., Feng, G. et al. Repurposing CRISPR-Cas12b for mammalian genome engineering. Cell Discov 4, 63 (2018) doi:10.1038/s41421-018-0069-4 Vector Backbone:pYPQ141-ZmUbi-RZ-Lb; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin 2026-08-15 01:06:05 0
pYPQ141-ZmUbi-RZ-Aa3.8.3
 
Resource Report
Resource Website 		RRID:Addgene_136374 Spectinomycin PMID:32170285 sgRNA scaffold and MS2 sequence are from: Teng, F., Cui, T., Feng, G. et al. Repurposing CRISPR-Cas12b for mammalian genome engineering. Cell Discov 4, 63 (2018) doi:10.1038/s41421-018-0069-5 Vector Backbone:pYPQ141-ZmUbi-RZ-Lb; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin 2026-08-15 01:06:05 0
pYPQ141-ZmUbi-RZ-Aac.3
 
Resource Report
Resource Website 		RRID:Addgene_136377 Spectinomycin PMID:32170285 Vector Backbone:pYPQ141-ZmUbi-RZ-Lb; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin 2026-08-15 01:06:05 0
pYPQ239A (dFnCas12a)-TV
 
Resource Report
Resource Website 		RRID:Addgene_136379 dFnCas12a-TV Francisella novicida U112 Spectinomycin PMID:32170285 TV activation system is from: Li, Z., Zhang, D., Xiong, X. et al. A potent Cas9-derived gene activator for plant and mammalian cells. Nature Plants 3, 930–936 (2017) doi:10.1038/s41477-017-0046-0 Vector Backbone:pYPQ167; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin D917A 2026-08-15 01:06:05 0
pYPQ292 (AaCas12b)-D570A-TV-MS2-TV
 
Resource Report
Resource Website 		RRID:Addgene_136381 AaCas12b (D570A)-TV-T2A-MCP-TV Alicyclobacillus acidiphilus Spectinomycin PMID:32170285 TV activation system is from: Li, Z., Zhang, D., Xiong, X. et al. A potent Cas9-derived gene activator for plant and mammalian cells. Nature Plants 3, 930–936 (2017) doi:10.1038/s41477-017-0046-2 Vector Backbone:pYPQ167; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin D570A 2026-08-15 01:06:05 0
pYPQ292 (AaCas12b)-D570A-TV-MS2-VPR
 
Resource Report
Resource Website 		RRID:Addgene_136382 AaCas12b (D570A)-TV-T2A-MCP-VPR Alicyclobacillus acidiphilus Spectinomycin PMID:32170285 TV activation system is from: Li, Z., Zhang, D., Xiong, X. et al. A potent Cas9-derived gene activator for plant and mammalian cells. Nature Plants 3, 930–936 (2017) doi:10.1038/s41477-017-0046-3; VPR activation system is from: Chavez A, Scheiman J, Vora S, Pruitt BW, Tuttle M, E PRI, et al. Highly efficient Cas9-mediated transcriptional programming. Nat Methods. 2015;12(4):326–8. 10.1038/nmeth.3312 Vector Backbone:pYPQ167; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin D570A 2026-08-15 01:06:05 0
pCL52.2
 
Resource Report
Resource Website 		RRID:Addgene_13656 Spectinomycin PMID:9045821 Backbone Size:7912; Vector Backbone:N/A; Vector Types:E. coli-S. arueus shuttle vector; Bacterial Resistance:Spectinomycin 2026-08-15 01:06:07 0
pED14xR-3
 
Resource Report
Resource Website 		RRID:Addgene_134798 R-TAGA-Evo1 qtRNA Synthetic Spectinomycin PMID:34588441 The lacI repressor must also be expressed in order to make the ProK-LacI promoter IPTG-inducible. These can be provided by the host strain, or on another plasmid. For example, E coli S2060s (Addgene Bacterial strain #105064), encode lacI. Vector Backbone:RK2 SpecR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin 2026-08-15 01:05:48 0
pED14xS-11
 
Resource Report
Resource Website 		RRID:Addgene_134803 Wildtype S tRNA (decodes TCG) Synthetic Spectinomycin PMID:34588441 The lacI repressor must also be expressed in order to make the ProK-LacI promoter IPTG-inducible. These can be provided by the host strain, or on another plasmid. For example, E coli S2060s (Addgene Bacterial strain #105064), encode lacI. Vector Backbone:RK2 SpecR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin 2026-08-15 01:05:48 0
pED14xS-WT
 
Resource Report
Resource Website 		RRID:Addgene_134804 Wildtype S tRNA (decodes TCG) Synthetic Spectinomycin PMID:34588441 The lacI repressor must also be expressed in order to make the ProK-LacI promoter IPTG-inducible. These can be provided by the host strain, or on another plasmid. For example, E coli S2060s (Addgene Bacterial strain #105064), encode lacI. Vector Backbone:RK2 SpecR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin 2026-08-15 01:05:48 0
pED14xW-1
 
Resource Report
Resource Website 		RRID:Addgene_134805 W-TAGA qtRNA Synthetic Spectinomycin PMID:34588441 The lacI repressor must also be expressed in order to make the ProK-LacI promoter IPTG-inducible. These can be provided by the host strain, or on another plasmid. For example, E coli S2060s (Addgene Bacterial strain #105064), encode lacI. Vector Backbone:RK2 SpecR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin 2026-08-15 01:05:48 0
pED14xY-3
 
Resource Report
Resource Website 		RRID:Addgene_134808 Y-TAGA-Evo1 qtRNA Synthetic Spectinomycin PMID:34588441 The lacI repressor must also be expressed in order to make the ProK-LacI promoter IPTG-inducible. These can be provided by the host strain, or on another plasmid. For example, E coli S2060s (Addgene Bacterial strain #105064), encode lacI. Vector Backbone:RK2 SpecR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin 2026-08-15 01:05:48 0
pTF101.1
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_134770 Spectinomycin Contains the 564bp bialaphos resistance (bar) gene from Streptomyces hygroscopicus Backbone Size:6742; Vector Backbone:pPZP200; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:05:48 2
pTF101.2
 
Resource Report
Resource Website 		RRID:Addgene_134771 Spectinomycin PMID:16249869 Contains the 564bp bialaphos resistance (bar) gene from Streptomyces hygroscopicus Backbone Size:6742; Vector Backbone:pPZP200; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:05:47 0
pH9-ilvA-acp (DE2)
 
Resource Report
Resource Website 		RRID:Addgene_135163 ilvA-acp Synthetic Spectinomycin PMID:31395784 Backbone Size:3000; Vector Backbone:pH9; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin 2026-08-15 01:05:51 0
CI-2
 
Resource Report
Resource Website 		RRID:Addgene_135165 CysJ-infA overlapping region in CI-2 Synthetic Spectinomycin PMID:31395784 Backbone Size:3100; Vector Backbone:pH9; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin 2026-08-15 01:05:51 0

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