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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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gRNA-trp-HYB Resource Report Resource Website 1+ mentions |
RRID:Addgene_64331 | gBlock product of trp1 deletion gRNA cassette | Synthetic | Ampicillin | PMID:25281382 | Note: Addgene's quality control sequencing finds one mismatch in the SNR52 promoter region, but plasmid function is not impacted. | Backbone Size:6217; Vector Backbone:pRS42H; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:10 | 2 | |
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gRNA-ura-HYB Resource Report Resource Website 1+ mentions |
RRID:Addgene_64330 | gBlock product of ura3 deletion gRNA cassette | Synthetic | Ampicillin | PMID:25281382 | Backbone Size:6217; Vector Backbone:pRS42H; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:10 | 5 | ||
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pU6-(BbsI)_CBh-Cas9-T2A-mcherry-P2A-Ad4E1B Resource Report Resource Website 1+ mentions |
RRID:Addgene_64221 | Cas9 | Streptococcus pyogenes | Ampicillin | PMID:25803306 | Please note that due to the presence of BbsI sites in the Ad4 coding regions it is NOT possible to directly clone new sgRNA sequences into the BbsI sites downstream of the U6 promoter. Therefore, precloned U6-sgRNA cassettes, isolated as 1.7 kb PvuI-XbaI fragments from pU6-(BbsI)_CBh-Cas9-T2A-BFP (plasmid# 64323) or pU6-(BbsI)_CBh-Cas9-T2A-mCherry (plasmid# 64324) of this deposit, or any other plasmid derived from pX330 (plasmid# 42230), must be ligated into the PvuI-XbaI digested backbone of this Ad4 containing plasmid. | Backbone Marker:Kuhn lab (Addgene plasmid # 64324); Vector Backbone:pU6-(BbsI)_CBh-Cas9-T2A-mCherry; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:09 | 1 | |
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pTet-IRES-EGFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_64238 | Ampicillin | PMID:25721206 | The transgene of interest can be inserted into BamHI, PmeI, and PstI sites. However, only the PmeI and PstI sites are recommended to be used for different restriction end ligation. This plasmid must be used together with an independent tTA-expressing plasmid for Tet-Off system or rtTA-expressing plasmid for Tet-On system. The TetO promoter sequence was excised from pETE-Bsd plasmid vector, which was originated from pUHD-10-3. The pETE-Bsd and pUHD-10-13 were engineered by Protopopov et al. and Damke et al, respectively. Protopopov AI, Li J, Winberg G, Gizatullin RZ, Kashuba VI, Klein G, et al. Human cell lines engineered for tetracycline-regulated expression of tumor suppressor candidate genes from a frequently affected chromosomal region, 3p21. J Gene Med. 2002/07/19 ed. 2002;4(4):397–406. Damke, H., Gossen, M., Freundlieb, S., Bujard, H., and Schmid, S.L. (1995). Tightly regulated and inducible expression of dominant interfering dynamin mutant in stably transformed HeLa cells. Methods Enzymol. 257, 209–220. | Backbone Marker:Didier Trono; Backbone Size:11101; Vector Backbone:pWPI; Vector Types:Mammalian Expression, Lentiviral, Cre/Lox; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:09 | 5 | |||
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pME-Cas9 Resource Report Resource Website 1+ mentions |
RRID:Addgene_64237 | codon optimized Cas9 | S. pyogenes | Kanamycin | PMID:25855067 | Backbone Marker:Chi-Bin Chien lab; Backbone Size:2765; Vector Backbone:pME-MCS; Vector Types:CRISPR; Bacterial Resistance:Kanamycin | 2026-08-15 01:18:10 | 2 | ||
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pGGDestTol2LC-1sgRNA Resource Report Resource Website 1+ mentions |
RRID:Addgene_64239 | Chloramphenicol and Ampicillin | PMID:25855067 | Backbone Size:7083; Vector Backbone:pDestTol2LC; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin | 2026-08-15 01:18:09 | 1 | ||||
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pMSCV-PIG-miR-146a Resource Report Resource Website 1+ mentions |
RRID:Addgene_64234 | miR146a | Homo sapiens | Ampicillin | PMID:18066065 | Backbone Marker:Scott Lowe; Backbone Size:7658; Vector Backbone:MSCV PIG; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:10 | 1 | ||
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pU6d:sgRNA#5 Resource Report Resource Website 1+ mentions |
RRID:Addgene_64249 | Spectinomycin | PMID:25855067 | Backbone Marker:Adam Bogdanove, Daniel Voytas lab; Backbone Size:2568; Vector Backbone:pLR-NN; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin | 2026-08-15 01:18:10 | 1 | ||||
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pU6c:sgRNA#4 Resource Report Resource Website 1+ mentions |
RRID:Addgene_64248 | Spectinomycin | PMID:25855067 | Backbone Marker:Adam Bogdanove, Daniel Voytas lab; Backbone Size:2568; Vector Backbone:pLR-NN; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin | 2026-08-15 01:18:10 | 4 | ||||
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pGGDestTol2LC-2sgRNA Resource Report Resource Website 1+ mentions |
RRID:Addgene_64240 | Chloramphenicol and Ampicillin | PMID:25855067 | Backbone Size:7083; Vector Backbone:pDestTol2LC; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin | 2026-08-15 01:18:10 | 1 | ||||
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pGGDestTol2LC-5sgRNA Resource Report Resource Website 1+ mentions |
RRID:Addgene_64243 | Chloramphenicol and Ampicillin | PMID:25855067 | Backbone Size:7083; Vector Backbone:pDestTol2LC; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin | 2026-08-15 01:18:10 | 1 | ||||
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MSGV Hu Acceptor Resource Report Resource Website 1+ mentions |
RRID:Addgene_64269 | Ampicillin | PMID:25757546 | Backbone Marker:Richard Morgan NCI; Backbone Size:6700; Vector Backbone:pMSGV; Vector Types:Retroviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:10 | 5 | ||||
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MSGV Rh Acceptor Resource Report Resource Website 1+ mentions |
RRID:Addgene_64271 | Ampicillin | PMID:25757546 | Backbone Marker:Richard Morgan NCI; Backbone Size:6700; Vector Backbone:pMSGV; Vector Types:Retroviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:10 | 1 | ||||
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pTPGI_dCas9 Resource Report Resource Website 1+ mentions |
RRID:Addgene_64279 | Yeast-optimized dCas9 | Saccharomyces cerevisiae | Ampicillin | PMID:23977949 | Vector Backbone:pRS304; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | D10A & H840A relative to S. pyogenes Cas9 | 2026-08-15 01:18:10 | 2 | |
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pcDNA3.1 TEV (full-length) Resource Report Resource Website 1+ mentions |
RRID:Addgene_64276 | Tobacco etch virus (TEV) protease | Synthetic | Ampicillin | PMID:25733847 | Backbone Size:5440; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:10 | 3 | ||
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myr-FLAG-PIK3CA-pcw107 Resource Report Resource Website 1+ mentions |
RRID:Addgene_64605 | PIK3CA | Bos taurus | Ampicillin | PMID:25538079 | AGGG (N-term barcode, 17 nt upstream of CDS start). Working name: CTK-B1-STOP | Backbone Marker:John Doench/Kathleen Ottina; Backbone Size:9240; Vector Backbone:pcw107 (V5); Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:12 | 1 | |
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MEK1 (S218D,S222D)-pcw107 Resource Report Resource Website 1+ mentions |
RRID:Addgene_64604 | MAP2K1 | Homo sapiens | Ampicillin | PMID:25538079 | ATGA (N-term barcode, 17 nt upstream of CDS start). Working name: CTK-A3-V5 | Backbone Marker:John Doench/Kathleen Ottina; Backbone Size:9240; Vector Backbone:pcw107 (V5); Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | S218D, S222D | 2026-08-15 01:18:11 | 3 |
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myr-FLAG-AKT1-pcw107 Resource Report Resource Website 1+ mentions |
RRID:Addgene_64606 | AKT1 | Homo sapiens | Ampicillin | PMID:25538079 | GAGG (N-term barcode, 17 nt upstream of CDS start). Working name: CTK-B2-STOP | Backbone Marker:John Doench/Kathleen Ottina; Backbone Size:9240; Vector Backbone:pcw107 (V5); Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:11 | 5 | |
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pcDNA3-TfR-OVA Resource Report Resource Website 1+ mentions |
RRID:Addgene_64600 | Ovalbumin | Gallus gallus | Ampicillin | PMID:11313828 | Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:11 | 3 | ||
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Hras (G12V)-pcw107 Resource Report Resource Website 1+ mentions |
RRID:Addgene_64603 | HRAS | Homo sapiens | Ampicillin | PMID:25538079 | AGTA (N-term barcode, 17 nt upstream of CDS start). Working name: CTK-A2-STOP | Backbone Marker:John Doench/Kathleen Ottina; Backbone Size:9240; Vector Backbone:pcw107 (V5); Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | G12V | 2026-08-15 01:18:11 | 1 |
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