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Species: Homo sapiens
Genetic Insert: STAT3
Vector Backbone Description: Backbone Marker:John Doench/Kathleen Ottina; Backbone Size:9240; Vector Backbone:pcw107 (V5); Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25538079
Comments: CAGA (N-term barcode, 17 nt upstream of CDS start). Working name: CTK-D2-V5
Proper citation: RRID:Addgene_64611 Copy
Species: Homo sapiens
Genetic Insert: GSK3B
Vector Backbone Description: Backbone Marker:John Doench/Kathleen Ottina; Backbone Size:9240; Vector Backbone:pcw107 (V5); Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25538079
Comments: AAGC (N-term barcode, 17 nt upstream of CDS start). Working name: CTK-E2-V5
Proper citation: RRID:Addgene_64614 Copy
Species: Homo sapiens
Genetic Insert: Centrin1
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pIRES Puro3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_64341 Copy
Species: Homo sapiens
Genetic Insert: Utrophin-mRFP
Vector Backbone Description: Vector Backbone:pIRES Puro3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_64358 Copy
Species: Homo sapiens
Genetic Insert: Limk1
Vector Backbone Description: Vector Backbone:pLVX-EF1-Cry2-mCherry; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_64368 Copy
Species: Homo sapiens
Genetic Insert: Cofilin1
Vector Backbone Description: Vector Backbone:pLVX-EF1-Cry2-mCherry; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_64370 Copy
Species: Homo sapiens
Genetic Insert: ATF1 Homology Arms
Vector Backbone Description: Backbone Size:5771; Vector Backbone:pFETCH_Donor; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26355004
Comments: This plasmid has been used along with PX548_ATF1_1 to add a FLAG tag to human ATF1 in HepG2 cells by the Mendenhall and Myers labs at HudsonAlpha Institute for Biotechnology/UAH Note: This plasmid is part of the Mendenhall and Meyers CRISPR-based Tagging system to add a tag (currently using FLAG) to endogenous proteins. Please see https://www.addgene.org/crispr/tagging/ for more details.
Proper citation: RRID:Addgene_64689 Copy
Species: Homo sapiens
Genetic Insert: WD40-encoding RNA antisense to P53
Vector Backbone Description: Backbone Size:4300; Vector Backbone:pCMV-TAG 2B; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25512560
Proper citation: RRID:Addgene_64680 Copy
Species: Homo sapiens
Genetic Insert: CTNNB1
Vector Backbone Description: Backbone Marker:John Doench/Kathleen Ottina; Backbone Size:9240; Vector Backbone:pcw107 (V5); Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25538079
Comments: TCAG (N-term barcode, 17 nt upstream of CDS start). Working name: CTK-E3-V5
Proper citation: RRID:Addgene_64616 Copy
Species: Homo sapiens
Genetic Insert: CTNNB1
Vector Backbone Description: Backbone Marker:John Doench/Kathleen Ottina; Backbone Size:9240; Vector Backbone:pcw107 (V5); Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25538079
Comments: TCAG (N-term barcode, 17 nt upstream of CDS start). Working name: CTK-E3-STOP
Proper citation: RRID:Addgene_64615 Copy
Species: Homo sapiens
Genetic Insert: MAPK14 (transcript variant 2)
Vector Backbone Description: Backbone Marker:John Doench/Kathleen Ottina; Backbone Size:9240; Vector Backbone:pcw107 (V5); Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25538079
Comments: CCAA (N-term barcode, 17 nt upstream of CDS start). Working name: CTK-I1-V5
Proper citation: RRID:Addgene_64624 Copy
Species: Homo sapiens
Genetic Insert: TP53 (transcript variant 3)
Vector Backbone Description: Backbone Marker:John Doench/Kathleen Ottina; Backbone Size:9240; Vector Backbone:pcw107 (V5); Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25538079
Comments: TGCA (N-term barcode, 17 nt upstream of CDS start). Working name: CTK-R1-V5
Proper citation: RRID:Addgene_64638 Copy
Species: Homo sapiens
Genetic Insert: AR (transcript variant 1, splice isoform)
Vector Backbone Description: Backbone Marker:John Doench/Kathleen Ottina; Backbone Size:9240; Vector Backbone:pcw107 (V5); Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25538079
Comments: GCTG (N-term barcode, 17 nt upstream of CDS start). Working name: CTK-P1-STOP
*Addgene sequence shows that there is an L57Q mutation in AR-V7 relative to NCBI sequences. There is also a "QQQ" insertion and a "GGGG" deletion relative to NM_000044.3. These mutations were in the original Sawyers lab clone and are not known to affect function.
Proper citation: RRID:Addgene_64635 Copy
Species: Homo sapiens
Genetic Insert: CASP8 (transcript variant B)
Vector Backbone Description: Backbone Marker:John Doench/Kathleen Ottina; Backbone Size:9240; Vector Backbone:pcw107 (V5); Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25538079
Comments: GATC (N-term barcode, 17 nt upstream of CDS start). Working name: CTK-M1-V5
Proper citation: RRID:Addgene_64632 Copy
Species: Homo sapiens
Genetic Insert: RALA
Vector Backbone Description: Backbone Marker:John Doench/Kathleen Ottina; Backbone Size:9240; Vector Backbone:pcw107 (V5); Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25538079
Comments: TTTT (N-term barcode, 17 nt upstream of CDS start). Working name: CTK-S3-full-V5
Proper citation: RRID:Addgene_64645 Copy
Species: Homo sapiens
Genetic Insert: RALA
Vector Backbone Description: Backbone Marker:John Doench/Kathleen Ottina; Backbone Size:9240; Vector Backbone:pcw107 (V5); Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25538079
Comments: TTTT (N-term barcode, 17 nt upstream of CDS start). Working name: CTK-S3-full-STOP
Proper citation: RRID:Addgene_64644 Copy
Species: Homo sapiens
Genetic Insert: METTL18:PBC004-E03:C231318
Vector Backbone Description: Backbone Marker:Structural Genomics Consortium (Addgene Plasmid #26096) ; Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: http://www.thesgc.org/structures/4RFQ/
Proper citation: RRID:Addgene_64656 Copy
Species: Homo sapiens
Genetic Insert: YTHDF1:JMC02A-E04:C231829
Vector Backbone Description: Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: http://www.thesgc.org/structures/4RCI/
Proper citation: RRID:Addgene_64653 Copy
Species: Homo sapiens
Genetic Insert: MAP2K1
Vector Backbone Description: Backbone Marker:John Doench/Kathleen Ottina; Backbone Size:9240; Vector Backbone:pcw107 (V5); Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25538079
Comments: TCTC (N-term barcode, 17 nt upstream of CDS start). Working name: CTK-X3-V5
Proper citation: RRID:Addgene_64650 Copy
Species: Homo sapiens
Genetic Insert: SIAH1
Vector Backbone Description: Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: http://www.thesgc.org/structures/4X3G/
Proper citation: RRID:Addgene_64663 Copy
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