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Vector Backbone Description: Backbone Marker:Self made; Derived from pBluescriptSK; Backbone Size:2804; Vector Backbone:pUPD; Vector Types:Synthetic Biology, Domestication of DNA parts for GoldenBraid asembly; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31836712
Comments: This is a GB2.0 domesticator vector. GBParts can be assembled in a BsmBI-ligation reaction. Any assembled Part can be released with BsaI for further cloning into Alpha level vectors.
Proper citation: RRID:Addgene_118043 Copy
Species: Synthetic
Genetic Insert: TRE-dDIO-vCre
Vector Backbone Description: Vector Backbone:pAAV2; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30455464
Comments: Note: vCre contains an A347T mutation. This mutation is not known to affect plasmid function.
Proper citation: RRID:Addgene_118029 Copy
Species: Homo sapiens
Genetic Insert: sgTP53-5
Vector Backbone Description: Backbone Marker:Feng Zhang Lab; Backbone Size:9482; Vector Backbone:pXPR003; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30224644
Comments: High efficiency of TP53 deletion
Proper citation: RRID:Addgene_118023 Copy
Species: Homo sapiens
Genetic Insert: sgTP53-4
Vector Backbone Description: Backbone Marker:Feng Zhang Lab; Backbone Size:9482; Vector Backbone:pXPR003; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30224644
Comments: High efficiency of TP53 deletion
Proper citation: RRID:Addgene_118022 Copy
Species: Synthetic
Genetic Insert: 2xNLS-mTurquoise2
Vector Backbone Description: Backbone Size:4561; Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30626963
Proper citation: RRID:Addgene_118025 Copy
Species: Staphylococcus epidermidis
Genetic Insert: SdrG_N2N3-B1-B2
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5216; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30420680
Proper citation: RRID:Addgene_117979 Copy
Species: Synthetic
Genetic Insert: TRE-vDIO-mScarlet-IRES-tTA
Vector Backbone Description: Vector Backbone:pAAV2; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30455464
Proper citation: RRID:Addgene_118030 Copy
Species: Synthetic
Genetic Insert: eGFP
Vector Backbone Description: Backbone Size:4935; Vector Backbone:pSIRV; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26780292
Proper citation: RRID:Addgene_118031 Copy
Genetic Insert: sfTq2ox-PBP5
Vector Backbone Description: Vector Backbone:pTHV037; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30648295
Proper citation: RRID:Addgene_117960 Copy
Species: Homo sapiens
Genetic Insert: TP53
Vector Backbone Description: Backbone Marker:Broad Institute; Backbone Size:8767; Vector Backbone:pLX313; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30224644
Proper citation: RRID:Addgene_118015 Copy
Species: S. pyrogenes
Genetic Insert: Cas9
Vector Backbone Description: Backbone Marker:Broad Institute; Backbone Size:8767; Vector Backbone:pLX311; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30224644
Proper citation: RRID:Addgene_118018 Copy
Species: P. pyralis
Genetic Insert: Firefly luciferase
Vector Backbone Description: Backbone Marker:Broad Institute; Backbone Size:8767; Vector Backbone:pLX313; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30224644
Proper citation: RRID:Addgene_118017 Copy
Species: Mus musculus
Genetic Insert: NFAT2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2400; Vector Backbone:pENTR11; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_11793 Copy
Species: Fusarium oxysporum f.sp lycopersici
Genetic Insert: dspAvr2
Vector Backbone Description: Vector Backbone:pZK538; Vector Types:Bacterial Expression, Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29481865
Comments: Original backbone received from Xiaorong Tao, Nanjing Agricultural University. Described in: https://doi.org/10.1371/journal.ppat.1005443 (Feng et al, 2016)
Proper citation: RRID:Addgene_118011 Copy
Vector Backbone Description: Backbone Size:7650; Vector Backbone:pLL3.7; Vector Types:Mammalian Expression, Lentiviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12590264
Comments: Lentiviral vector that expresses shRNA under the mouse U6 promoter. A CMV-EGFP reporter cassette is included in the vector to monitor expression.
For protocols regarding the use of pLL3.7, visit: http://web.mit.edu/jacks-lab/protocols/rnairesources.htm
Note: A single base pair deletion at position 11 of the U6 promoter in this plasmid does not impair the efficacy of this reagent.
Proper citation: RRID:Addgene_11795 Copy
Species: Homo sapiens
Genetic Insert: TP53
Vector Backbone Description: Backbone Marker:Broad Institute; Backbone Size:8767; Vector Backbone:pLX313; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30224644
Proper citation: RRID:Addgene_118014 Copy
Genetic Insert: JS200
Vector Backbone Description: Backbone Size:0; Vector Backbone:n/a; Vector Types:; Bacterial Resistance:None
Defining Citation: PMID:12909725
Comments: For use with pEP PolI (addgene #11722) and pWT PolI (addgene #11721). This strain contains a temp sens mutation in PolI.
Proper citation: RRID:Addgene_11794 Copy
Species: Fusarium oxysporum f.sp lycopersici
Genetic Insert: dspSix5
Vector Backbone Description: Vector Backbone:pGWB402; Vector Types:Bacterial Expression, Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29481865
Comments: Original backbone received from Tsuyoshi Nakagawa, Shimane University. Described in: https://doi.org/10.1271/bbb.70216 (Nakagawa et al, 2007)
Proper citation: RRID:Addgene_118013 Copy
Species: Synthetic
Genetic Insert: RBS, amilGFP
Vector Backbone Description: Backbone Marker:iGEM Registry; Vector Backbone:BioBrick pSB1C3 vector; Vector Types:Synthetic Biology, Escherichia coli; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29760772
Proper citation: RRID:Addgene_118006 Copy
Genetic Insert: sfTq2
Vector Backbone Description: Vector Backbone:c1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30648295
Proper citation: RRID:Addgene_117932 Copy
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