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Species: Mus musculus
Genetic Insert: p53
Vector Backbone Description: Backbone Size:5846; Vector Backbone:pBKWH; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30217970
Proper citation: RRID:Addgene_133900 Copy
Species: Other
Genetic Insert: ER_K0
Vector Backbone Description: Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31595041
Proper citation: RRID:Addgene_133863 Copy
Species: Other
Genetic Insert: Cyto_K20
Vector Backbone Description: Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31595041
Proper citation: RRID:Addgene_133862 Copy
Species: Other
Genetic Insert: ER_K20
Vector Backbone Description: Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31595041
Proper citation: RRID:Addgene_133861 Copy
Species: Homo sapiens
Genetic Insert: CD86
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31606123
Proper citation: RRID:Addgene_133860 Copy
Species: Homo sapiens
Genetic Insert: GNG2
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31606123
Proper citation: RRID:Addgene_133857 Copy
Species: Homo sapiens
Genetic Insert: HRas
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:C3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31606123
Proper citation: RRID:Addgene_133855 Copy
Species: Homo sapiens
Genetic Insert: HRas
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:C3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31606123
Proper citation: RRID:Addgene_133854 Copy
Species: Other
Genetic Insert: EGFP
Vector Backbone Description: Vector Backbone:pEGFP-N1 (clontech); Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31679934
Proper citation: RRID:Addgene_133929 Copy
Species: Homo sapiens
Genetic Insert: SUMO-conjugating enzyme UBC9
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5368; Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32929246
Proper citation: RRID:Addgene_133911 Copy
Species: Homo sapiens
Genetic Insert: SUMO-conjugating enzyme UBC9
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5368; Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32929246
Proper citation: RRID:Addgene_133910 Copy
Vector Backbone Description: Backbone Size:0; Vector Backbone:pShuttle; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Comments: pShuttle is used to make adenovirus in the AdEasy system. The CAG promoter was inserted for stronger expression and a MCS compatible with pBS.
See author's map for more information.
Proper citation: RRID:Addgene_13427 Copy
Genetic Insert: RNAP alpha-Gal4
Vector Backbone Description: Backbone Size:3600; Vector Backbone:pACLU-Kan; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17406455
Comments: Expresses E. coli RNAP alpha subunit aa 1-248 fused to yeast Gal4 aa 58-97.
This plasmid requires a bacterial strain, such as XL1 Blue, that expresses the lacIq allele of lac repressor. This ensures repression of the strong lacUV5 promoter.
Proper citation: RRID:Addgene_13421 Copy
Species: Homo sapiens
Genetic Insert: mCherry-HJURP-LacI 1-304
Vector Backbone Description: Vector Backbone:pkg196; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_134227 Copy
Species: Homo sapiens
Genetic Insert: DK360 tdTomato (long SKAP)
Vector Backbone Description: Backbone Marker:Erik Dent (Addgene plasmid # 50708); Vector Backbone:EB3-tdTomato; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_134229 Copy
Species: Synthetic
Genetic Insert: kanR_gp41-1 N-intein + kanR_gp41-1 C-intein
Vector Backbone Description: Vector Backbone:pBAD33 + pTrc99a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31672972
Comments: pSiMPlk_N should be cotransformed with pSiMPlk_C (or vice versa) in E. coli to get a reconstituted functional APT thereby conferring resistance against kanamycin. mRuby3 gene was amplified from a construct bought by us from Addgene (ID: 74252).
Please see the Sequences link for the individual plasmid sequences.
The sample from Addgene will have both the plasmids pSiMPlk_N + pSiMPlk_C in it for the interested
antibiotic resistance. Separating individual plasmid backbones will be possible via PCR. Agarose gel electrophoresis can be used to visualize the presence of both the plasmids. But, separating individual plasmids via gel electrophoresis, in our hands, always resulted in cross-contamination of the two backbones.
Proper citation: RRID:Addgene_134310 Copy
Vector Backbone Description: Backbone Size:0; Vector Backbone:pShuttle; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Comments: pShuttle is used to make adenovirus in the AdEasy system. The human U6 promoter allows for siRNA expression.
Proper citation: RRID:Addgene_13428 Copy
Genetic Insert: W4-loxP-TT-loxP-mRFP1
Vector Backbone Description: Backbone Size:2432; Vector Backbone:pBbA-k; Vector Types:Bacterial Expression, Cre/Lox, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31961670
Comments: Please visit https://www.biorxiv.org/content/10.1101/786533v1 for bioRxiv preprint.
Please note: This plasmid exists as a multimer. This is not expected to impact the end function of the plasmid. Please refer to the Addgene Blog post on multimers for more information: https://blog.addgene.org/plasmids-101-dimers-and-multimers
Proper citation: RRID:Addgene_134405 Copy
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:pYTK096; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31433622
Proper citation: RRID:Addgene_134406 Copy
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:pYTK096; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31433622
Comments: The plasmid contains a small insertion 3' of the insert that results in the C-terminal NLS being unfused to the ORF.
Proper citation: RRID:Addgene_134410 Copy
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