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Species: Mus musculus
Genetic Insert: Clock
Vector Backbone Description: Backbone Marker:sigma; Vector Backbone:pCMV103Xflag; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22653727
Proper citation: RRID:Addgene_47340 Copy
Species: Mus musculus
Genetic Insert: BMAL
Vector Backbone Description: Vector Backbone:pfastbac; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22653727
Proper citation: RRID:Addgene_47335 Copy
Species: Mus musculus
Genetic Insert: Clock
Vector Backbone Description: Backbone Marker:sigma; Vector Backbone:pCMV103Xflag; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22653727
Proper citation: RRID:Addgene_47338 Copy
Species: Mus musculus
Genetic Insert: TTC30A2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5546; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23810713
Proper citation: RRID:Addgene_47324 Copy
Species: Mus musculus
Genetic Insert: TTC30B
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5546; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23810713
Proper citation: RRID:Addgene_47325 Copy
Species: Other
Genetic Insert: Cas9
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2; Vector Types:Mammalian Expression, CRISPR, xenopus and zebrafish expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24873830
Comments: Please see the Schier lab website http://labs.mcb.harvard.edu/schier/ for detailed protocols about target site selection, sgRNA production, stop codon cassette design, Cas9 protein purification, injection and downstream analysis.
This plasmid was used to prepare and inject Cas9 mRNA into zebrafish zygotes.
Proper citation: RRID:Addgene_47322 Copy
Species: Mus musculus
Genetic Insert: TTC30A1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5546; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23810713
Proper citation: RRID:Addgene_47323 Copy
Species: Other
Genetic Insert: minCMV-dTomato_pA
Vector Backbone Description: Vector Backbone:pcrBluntII-topo; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23907171
Proper citation: RRID:Addgene_47321 Copy
Species: Other
Genetic Insert: Cas9m2
Vector Backbone Description: Vector Backbone:pcDNA3.3_TOPO; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23907171
Proper citation: RRID:Addgene_47314 Copy
Species: Other
Genetic Insert: wild type immunity region of lambda phage with tsr-venus-ub-cI
Vector Backbone Description: Backbone Marker:NEB; Backbone Size:4225; Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22751020
Proper citation: RRID:Addgene_47311 Copy
Species: Other
Genetic Insert: Cas9m2-VP64
Vector Backbone Description: Vector Backbone:pcDNA3.3_TOPO; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23907171
Proper citation: RRID:Addgene_47317 Copy
Species: Other
Genetic Insert: Cas9m3-VP64
Vector Backbone Description: Vector Backbone:pcDNA3.3_TOPO; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23907171
Proper citation: RRID:Addgene_47318 Copy
Species: Other
Genetic Insert: Cas9m3
Vector Backbone Description: Vector Backbone:pcDNA3.3_TOPO; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23907171
Proper citation: RRID:Addgene_47315 Copy
Species: Homo sapiens
Genetic Insert: Intersectin short
Vector Backbone Description: Backbone Size:4754; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11584276
Proper citation: RRID:Addgene_47392 Copy
Species: Homo sapiens
Genetic Insert: Clavesin1
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4324; Vector Backbone:pCMV-Tag2B; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19651769
Proper citation: RRID:Addgene_47430 Copy
Species: Other
Genetic Insert: Intersectin SH3 domain C
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:5000; Vector Backbone:pGEX-2t; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9813051
Proper citation: RRID:Addgene_47398 Copy
Species: Homo sapiens
Genetic Insert: intersectin long
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11584276
Proper citation: RRID:Addgene_47395 Copy
Species: Other
Genetic Insert: Intersectin SH3 domain A
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:5000; Vector Backbone:pGEX-2t; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9813051
Proper citation: RRID:Addgene_47396 Copy
Species: Mus musculus
Genetic Insert: KIF17-short
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5546; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: This vector directs expression of neither of the current mouse RefSeq Kif17 variants, isoform 1 or isoform 2. Rather the vector expresses a cDNA which corresponds to a different alternatively spliced transcript encoding a still shorter isoform of 511 amino acids rather than the 1038 residues of isoform 1 or 710 of isoform 2. The first 400 residues of the “short” isoform are essentially identical to the amino-terminal motor domain of isoform 1 and 2. Based on studies of Kif17 truncations, the short form of mouse Kif17 is predicted to form dimers and to bind microtubules.
*Compared to the reference sequence, this expression vector expresses a protein with substitution of conserved glycine-152 with a serine in the motor domain of Kif17. Due to this mutation, the properties of the produced protein could be altered.
Proper citation: RRID:Addgene_47545 Copy
Species: Caenorhabditis elegans
Genetic Insert: Glutamate-gated chloride channels
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2037; Vector Backbone:pcDNA3.1/V5-His TOPO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23720773
Proper citation: RRID:Addgene_47542 Copy
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