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Species: Other
Genetic Insert: Integrase 4 coding sequence codon optimized for A. thaliana expression.
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32444777
Comments: The whole insert (promoter, CDS and terminator) was synthesized and cloned into backbone plasmid. The Actin 2 promoter came from Aragao, F. J. L., et al. Plant Science 168, 1227-1233 (2005) and the NOS terminator sequence from pBI426 plasmid of Datla, R.S., et al. Gene 101, 239-246 (1991). The original integrase 4 coding sequence (before A. thaliana codon optimization) was obtained from Yang, L. et al. Nat. Methods 11, 1261-1266 (2014).
Proper citation: RRID:Addgene_127530 Copy
Species: Synthetic
Genetic Insert: OphA-delta C6-R72K
Vector Backbone Description: Vector Backbone:pET24; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30151425
Proper citation: RRID:Addgene_127683 Copy
Species: Synthetic
Genetic Insert: OphA-delta C6-Y66F
Vector Backbone Description: Vector Backbone:pET24; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30151425
Proper citation: RRID:Addgene_127681 Copy
Species: Synthetic
Genetic Insert: OphA-delta C6-Y63F
Vector Backbone Description: Vector Backbone:pET24; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30151425
Proper citation: RRID:Addgene_127680 Copy
Species: Synthetic
Genetic Insert: OphA-delta C6-Y98F
Vector Backbone Description: Vector Backbone:pET24; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30151425
Proper citation: RRID:Addgene_127686 Copy
Species: Synthetic
Genetic Insert: OphA-delta C6-Y76F
Vector Backbone Description: Vector Backbone:pET24; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30151425
Proper citation: RRID:Addgene_127684 Copy
Species: Synthetic
Genetic Insert: OphA-delta C6-P399A
Vector Backbone Description: Vector Backbone:pET24; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30151425
Proper citation: RRID:Addgene_127689 Copy
Species: Synthetic
Genetic Insert: OphA-delta C6
Vector Backbone Description: Vector Backbone:pET24; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30151425
Proper citation: RRID:Addgene_127679 Copy
Species: Homo sapiens
Genetic Insert: NONO sgRNA
Vector Backbone Description: Vector Backbone:gRNA_Cloning Vector; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30270045
Proper citation: RRID:Addgene_127654 Copy
Species: Synthetic
Genetic Insert: RNA nanostrcutre
Vector Backbone Description: Backbone Size:3300; Vector Backbone:pSB4K5; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29564885
Comments: The insert was designed by F. Chizzolini and M. Schwarz-Schilling and cloned by M.Schwarz-Schilling. The RNA sequence for the: 3WJ is from Shu, D. et al. Nucleic Acids Res. 2014, 42, e10; MG aptamer is from Babendure, J. R. et al. J. Am. Chem. Soc. 2003, 125, 14716−7; PP7 aptamer is from Chao, J. A. et al. Nat. Struct. Mol. Biol. 2008, 15, 103−5; TAR aptamer is from Bayer, T. S. et al. RNA 2005, 11, 1848−57; Streptavidin aptamer is from: Srisawat, C. and Engelke, D. R. RNA 2001, 7, 632−41.
Proper citation: RRID:Addgene_127634 Copy
Species: Homo sapiens
Genetic Insert: Tom70
Vector Backbone Description: Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31894645
Proper citation: RRID:Addgene_127633 Copy
Species: Mus musculus
Genetic Insert: Trak1
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24671417
Proper citation: RRID:Addgene_127621 Copy
Species: Homo sapiens
Genetic Insert: Miro1
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31894645
Proper citation: RRID:Addgene_127628 Copy
Species: Homo sapiens
Genetic Insert: Miro1
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31894645
Proper citation: RRID:Addgene_127626 Copy
Species: Synthetic
Genetic Insert: cis-aconitate decarboxylase
Vector Backbone Description: Vector Backbone:pDSM-bc; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29753070
Comments: CAD transcription unit with pathway position 3 homology arms, compatible with 5' position 2 and 3' position 3 homology arm. Sequencing primers are also the primers for amplifying DNA for integration into the S. cerevisiae genome.
Proper citation: RRID:Addgene_127729 Copy
Species: Synthetic
Genetic Insert: cis-aconitate decarboxylase
Vector Backbone Description: Vector Backbone:pDSM-bc; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29753070
Comments: CAD transcription unit with pathway position 3 homology arms, compatible with 5' position 2 and 3' position 3 homology arm. Sequencing primers are also the primers for amplifying DNA for integration into the S. cerevisiae genome.
Proper citation: RRID:Addgene_127728 Copy
Species: Aspergillus terreus
Genetic Insert: cis-aconitate decarboxylase
Vector Backbone Description: Vector Backbone:pDSM-bc; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29753070
Comments: CAD transcription unit with pathway position 3 homology arms, compatible with 5' position 2 and 3' position 3 homology arm. Sequencing primers are also the primers for amplifying DNA for integration into the S. cerevisiae genome.
Proper citation: RRID:Addgene_127727 Copy
Species: Aspergillus terreus
Genetic Insert: itaconate transporter from A. terreus
Vector Backbone Description: Vector Backbone:pDSM-5a; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29753070
Comments: ITE transcription unit with pathway position 1 homology arms, compatible with 5' ChrXV homology arm (5int) and 3' position 2 homology arm. Sequencing primers are also the primers for amplifying DNA for integration into the S. cerevisiae genome.
Proper citation: RRID:Addgene_127725 Copy
Vector Backbone Description: Vector Backbone:pDSM; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29753070
Comments: Assembles a transcription unit and adds pathway position 4 homology arms, compatible with 5' position 3 and 3' position 5 homology arm. Ccdb selection is used to select for correct clones. Sequencing primers are also the primers for amplifying DNA for integration into the S. cerevisiae genome.
Proper citation: RRID:Addgene_127719 Copy
Species: Escherichia coli
Genetic Insert: ygiF-CHAD
Vector Backbone Description: Backbone Marker:Michael Hothorn; Backbone Size:5320; Vector Backbone:pMH-HT; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31133615
Proper citation: RRID:Addgene_127793 Copy
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