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Species: K.marxianus CBS 6556
Genetic Insert: promINU1
Vector Backbone Description: Backbone Marker:John Dueber, Addgene plasmid #65108; Backbone Size:2676; Vector Backbone:pYTK001; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:40629255
Comments: Please visit https://doi.org/10.1101/2025.02.03.636257 for bioRxiv preprint.
Proper citation: RRID:Addgene_233494 Copy
Species: Homo sapiens
Genetic Insert: sp_P59827_BPIB4_HUMAN:0-17
Vector Backbone Description: Backbone Marker:John Dueber, Addgene plasmid #65108; Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:39674781
Comments: Signal peptides direct recombinant protein secretion in yeast. Codon optimised for expression in S. cerevisiae. This part is designed for use with components from the MoClo Yeast Toolkit (Lee et al. 2015). This insert is a 3a* component, see Williams et al. 2024 for more detail.
Proper citation: RRID:Addgene_228192 Copy
Species: Homo sapiens
Genetic Insert: Chromogranin-A
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40458085
Comments: FRET sensor consisting of:
1-residue hCGA(1-115),
2-mCitrine,
3-EcoRI,
4-C-terminal cleavage site of CST,
5-BamHI,
6-mScarlet-I,
7-XbaI
Proper citation: RRID:Addgene_228572 Copy
Species: Homo sapiens
Genetic Insert: Chromogranin-A
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40458085
Comments: FRET sensor consisting of:
1-residue hCGA(1-115),
2-mCitrine,
3-EcoRI,
4-N-terminal cleavage site of CST,
5-BamHI,
6-mScarlet-I,
7-XbaI
Proper citation: RRID:Addgene_228573 Copy
Species: Homo sapiens
Genetic Insert: sp_P13500_CCL2_HUMAN:0-23
Vector Backbone Description: Backbone Marker:John Dueber, Addgene plasmid #65108; Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:39674781
Comments: Signal peptides direct recombinant protein secretion in yeast. Codon optimised for expression in S. cerevisiae. This part is designed for use with components from the MoClo Yeast Toolkit (Lee et al. 2015). This insert is a 3a* component, see Williams et al. 2024 for more detail.
Proper citation: RRID:Addgene_228178 Copy
Species: K.marxianus CBS 6556
Genetic Insert: promGLK1A
Vector Backbone Description: Backbone Marker:John Dueber, Addgene plasmid #65108; Backbone Size:2676; Vector Backbone:pYTK001; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:40629255
Comments: Please visit https://doi.org/10.1101/2025.02.03.636257 for bioRxiv preprint.
Proper citation: RRID:Addgene_233482 Copy
Species: Rattus norvegicus
Genetic Insert: SpotNES-sGFP2-SynP3-3xmyc
Vector Backbone Description: Backbone Size:5481; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40947561
Comments: Please visit https://doi.org/10.1101/2025.05.07.652623 for bioRxiv preprint.
Depositor confirms P89S in sGFP2, changes are not expected to impact function.
Proper citation: RRID:Addgene_239623 Copy
Species: Engineered reporter (from Promega)
Genetic Insert: Secreted nanoluciferase, with signal peptide from PMEL (gp100), flanked by human haemoglobin beta 5' and 3' UTRs
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3636; Vector Backbone:pGL3Control; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40661784
Proper citation: RRID:Addgene_239862 Copy
Vector Backbone Description: Backbone Marker:John Dueber, Addgene plasmid #65202; Vector Backbone:pYTK095; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39674781
Comments: Empty vector for protein expression in S. cerevisiae.
Proper citation: RRID:Addgene_228185 Copy
Species: K.marxianus CBS 6556
Genetic Insert: promRPE1
Vector Backbone Description: Backbone Marker:John Dueber, Addgene plasmid #65108; Backbone Size:2676; Vector Backbone:pYTK001; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:40629255
Comments: Please visit https://doi.org/10.1101/2025.02.03.636257 for bioRxiv preprint.
Proper citation: RRID:Addgene_233486 Copy
Species: Haemophilus influenzae
Genetic Insert: Hia5
Vector Backbone Description: Backbone Marker:ATUM Bio; Backbone Size:2260; Vector Backbone:pD861; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:40161611
Comments: For a protocol for the purification of the proteins, please visit https://www.protocols.io/view/purification-and-activity-testing-for-nanobody-hia-6qpvrqbyzlmk/v1.
Please visit https://doi.org/10.1101/2025.03.11.642717 for bioRxiv preprint.
Plasmids also referenced in https://doi.org/10.1101/2025.07.01.662447.
Proper citation: RRID:Addgene_239626 Copy
Species: Homo sapiens
Genetic Insert: Full premelanosome coding sequence with IL6 signal peptide, flanked by human haemoglobin beta 5' and 3' UTRs
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3636; Vector Backbone:pGL3Control; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40661784
Proper citation: RRID:Addgene_239868 Copy
Species: K.marxianus CBS 6556
Genetic Insert: promALD1
Vector Backbone Description: Backbone Marker:John Dueber, Addgene plasmid #65108; Backbone Size:2676; Vector Backbone:pYTK001; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:40629255
Comments: Please visit https://doi.org/10.1101/2025.02.03.636257 for bioRxiv preprint.
Proper citation: RRID:Addgene_233488 Copy
Species: Rattus norvegicus
Genetic Insert: PSD95.FingR-NES-SynP3-3xmyc
Vector Backbone Description: Backbone Size:6324; Vector Backbone:pLL3.7; Vector Types:Mammalian Expression, Lentiviral, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40947561
Comments: Please visit https://doi.org/10.1101/2025.05.07.652623 for bioRxiv preprint.
Proper citation: RRID:Addgene_239625 Copy
Species: Rattus norvegicus
Genetic Insert: PSD95.FingR-NES-SynP3-3xmyc
Vector Backbone Description: Backbone Size:6324; Vector Backbone:pLL3.7; Vector Types:Mammalian Expression, Lentiviral, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40947561
Comments: Please visit https://doi.org/10.1101/2025.05.07.652623 for bioRxiv preprint.
Proper citation: RRID:Addgene_239624 Copy
Species: Firefly
Genetic Insert: LUC
Vector Backbone Description: Backbone Marker:Tuttle et al Plant Methods. 2012 Aug 1;8(1):27. doi: 10.1186/1746-4811-8-27.; Vector Backbone:pJRT.Agro.CLCrVA.008; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:40394900
Proper citation: RRID:Addgene_234915 Copy
Species: Homo sapiens
Genetic Insert: PDZD8
Vector Backbone Description: Vector Backbone:pcDNA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38898113
Proper citation: RRID:Addgene_227856 Copy
Species: Homo sapiens
Genetic Insert: PDZD8
Vector Backbone Description: Vector Backbone:pGEX4T-1 ; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38898113
Proper citation: RRID:Addgene_227850 Copy
Genetic Insert: E. coli E69 (O9a:K30) Wzc with N711Y mutation
Vector Backbone Description: Backbone Size:4542; Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40210632
Proper citation: RRID:Addgene_217920 Copy
Species: Homo sapiens
Genetic Insert: KGA isoform
Vector Backbone Description: Vector Backbone:pcDNA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38898113
Proper citation: RRID:Addgene_227843 Copy
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