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Species: Homo sapiens
Genetic Insert: Human Granzyme A
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5360; Vector Backbone:pET26b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:9256474
Proper citation: RRID:Addgene_8823 Copy
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:6700; Vector Backbone:pMal-C2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8635750
Comments: This plasmid expression vector enables a protein of interest to be produced with a biotin acceptor peptide (BAP) fused directly to its N-terminus, or, alternatively, as a fusion to the C-terminus of E. coli maltose-binding protein with the BAP on its N-terminus. pDW363 also overproduces biotin holoenzyme synthetase (BirA) to ensure efficient biotinylation of BAP-tagged proteins in vivo. Add biotin to the medium (50 mM) when cultivating cells that contain pDW363 or it derivatives.
Proper citation: RRID:Addgene_8842 Copy
Species: tobacco etch virus
Genetic Insert: TEV protease, S219D mutant
Vector Backbone Description: Backbone Marker:Herman Bujard Lab; Backbone Size:6700; Vector Backbone:pZA31; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:10873547
Comments: This TEV protease expression vector is intended to be used for controlled intracellular processing of fusion proteins (TEV protease substrates) in E. coli. Expression of the TEV protease gene on pRK603 will be constitutive unless the host strain produces Tet repressor. Regulated expression can be achieved in DH5alphaPro or BL21Pro cells (Clontech).
Proper citation: RRID:Addgene_8831 Copy
Species: Mus musculus
Genetic Insert: PGC-1 alpha promoter dCRE
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3-basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12764228
Comments: 5' flanking sequence of mouse PGC-1 alpha gene PCR amplified between +78 and -2533 with respect to the transcriptional start site. Mutation of the CRE site between -146 and -129 from TGACGTCA to TAGATCTA.
NOTE: The full sequence from the depositor is theoretical. There are several discrepancies between Addgene's quality control sequence and the full sequence provided here, but those discrepancies do not appear to affect function. Several labs have published successfully with this plasmid.
Proper citation: RRID:Addgene_8888 Copy
Species: Mus musculus
Genetic Insert: SREBP-1c
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3160; Vector Backbone:pSV-Sport; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8336713
Comments: Note that this is a mouse SREBP-1c, while the paper reference is a rat SREBP-1c. SREBP-1c differs from SREBP1a over amino acids 3-5 and contains a deletion of amino acids 6-29. See Figure 1c of the associated publication for more information.
Addgene's sequencing results identified a deletion of aa90-131 of SREBP1c compared to GenBank sequence NP_035610.1.
Proper citation: RRID:Addgene_8883 Copy
Species: Homo sapiens
Genetic Insert: RXR alpha
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3160; Vector Backbone:pSV-Sport; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7926726
Proper citation: RRID:Addgene_8882 Copy
Species: Mus musculus
Genetic Insert: PGC-1 alpha promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3-basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12764228
Comments: 5' flanking sequence of mouse PGC-1 alpha gene PCR amplified between +78 and -2533 with respect to the transcriptional start site.
Proper citation: RRID:Addgene_8887 Copy
Species: Mus musculus
Genetic Insert: Trp53
Vector Backbone Description: Vector Backbone:pSpCas9n(BB)-2A-GFP (PX461); Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27889317
Proper citation: RRID:Addgene_88847 Copy
Species: Other
Genetic Insert: LifeAct
Vector Backbone Description: Vector Backbone:mEos4b-Lifeact Plasmid #54813; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_88840 Copy
Species: Mus musculus
Genetic Insert: PPAR gamma
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3160; Vector Backbone:pSV-Sport; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7926726
Proper citation: RRID:Addgene_8862 Copy
Species: Homo sapiens
Genetic Insert: Surfactant protein B gene promoter
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGL4.10; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22761434
Proper citation: RRID:Addgene_88868 Copy
Species: Mus musculus
Genetic Insert: aP2 enhancer / promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5010; Vector Backbone:pGL3-promoter; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:1545801
Comments: Plasmid created by James Rhee by cloning 520bp of the aP2 enhancer from aP2-CAT into the pGL2 luciferase reporter. This is the abbreviated insert sequence:
ggtaccGAATTCCAGCAGGAATCA.......GGATCAGAGTTCA (DR-1)......TTCTTCCTTCTAGActcgag
Proper citation: RRID:Addgene_8858 Copy
Species: Homo sapiens
Genetic Insert: hAR-C576A
Vector Backbone Description: Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8175737
Proper citation: RRID:Addgene_89108 Copy
Species: Rattus norvegicus
Genetic Insert: Fatty Acid Synthetase Promoter
Vector Backbone Description: Vector Backbone:n/a; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9421459
Comments: -220 to +25 fragment of the Fatty Acid Synthetase promoter with regard to the transcription start site.
Proper citation: RRID:Addgene_8890 Copy
Species: Aequorea Victoria
Genetic Insert: EmGFP
Vector Backbone Description: Backbone Size:2508; Vector Backbone:pEMBL8; Vector Types:Mammalian Expression, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28300165
Proper citation: RRID:Addgene_89060 Copy
Species: Synthetic
Genetic Insert: Z3EV promoter cassette
Vector Backbone Description: Backbone Marker:Bahler et al, Yeast, 14(10) 943-51, 1998; Backbone Size:5100; Vector Backbone:pFS6a-KanMX6-P3nmt1; Vector Types:Yeast integration vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28423198
Proper citation: RRID:Addgene_89066 Copy
Species: Synthetic
Genetic Insert: Z3EV promoter GFP-NLS leu1 C-terminal 300 nt
Vector Backbone Description: Backbone Marker:Apolinario, E., Nocero, M., Jin, M., & Hoffman, C. S. (1993). Current Genetics, 24(6), 491–495.; Backbone Size:5763; Vector Backbone:pBSSK-His7(F); Vector Types:S. pombe his7 integration vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28423198
Proper citation: RRID:Addgene_89065 Copy
Species: Synthetic
Genetic Insert: Z3EV artificial transcription factor
Vector Backbone Description: Backbone Marker:Rhind Lab; Backbone Size:5285; Vector Backbone:pFS181 (pSS1); Vector Types:leu1 integrating vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28423198
Proper citation: RRID:Addgene_89064 Copy
Species: Homo sapiens
Genetic Insert: Dystrophin
Vector Backbone Description: Vector Backbone:p37; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28139886
Proper citation: RRID:Addgene_88892 Copy
Species: Homo sapiens
Genetic Insert: hAR-538-614
Vector Backbone Description: Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8175737
Proper citation: RRID:Addgene_89107 Copy
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