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Species: Homo sapiens
Genetic Insert: Pak1
Vector Backbone Description: Backbone Size:4900; Vector Backbone:pCMV6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9395435
Comments: Created by 3-way ligation of PCR products: B/Sac-Sac/Eco-Eco/B.
Pak1 may contain mutations G401S and L516I. Depositor states that this should not affect plasmid function.
Proper citation: RRID:Addgene_12211 Copy
Species: Homo sapiens
Genetic Insert: Pak1
Vector Backbone Description: Backbone Size:4900; Vector Backbone:pCMV6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11719502
Comments: Addgene has found that this plasmid is extremely slow growing. We recommend keeping plates and liquid cultures growing for at least 20 hours. Overnight will not be sufficient.
There is also an additional mutation in this plasmid - L516I. The L516I mutation, or SNP, has been present from the very beginning (~1995). The crystal structure of Pak1 uses this clone, so it is present there too. The depositor does not think it affects function in any way.
Pak1 may contain a G401S mutation. Depositor states that this mutation should not affect plasmid function.
Proper citation: RRID:Addgene_12212 Copy
Species: Homo sapiens
Genetic Insert: DD-linker-M.EcoGII-V5-TERF1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5875; Vector Backbone:pRetroX-pTuner; Vector Types:Mammalian Expression, Bacterial Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30517874
Proper citation: RRID:Addgene_122084 Copy
Species: Homo sapiens
Genetic Insert: DD-linker-M.EcoGII-V5-LaminB1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5875; Vector Backbone:pRetroX-pTuner; Vector Types:Mammalian Expression, Bacterial Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30517874
Proper citation: RRID:Addgene_122083 Copy
Species: Homo sapiens
Genetic Insert: Brg1
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19342595
Proper citation: RRID:Addgene_122115 Copy
Species: Homo sapiens
Genetic Insert: Brg1
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19342595
Proper citation: RRID:Addgene_122117 Copy
Species: Homo sapiens
Genetic Insert: His6 MBP TEV HDAC8_374
Vector Backbone Description: Backbone Marker:Scott Gradia; Backbone Size:6465; Vector Backbone:pET His6 MBP TEV LIC cloning vector; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28846375
Proper citation: RRID:Addgene_122174 Copy
Species: Homo sapiens
Genetic Insert: POU2F3
Vector Backbone Description: Backbone Size:7183; Vector Backbone:LentiV_Neo; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29945888
Proper citation: RRID:Addgene_122175 Copy
Species: Homo sapiens
Genetic Insert: Coilin
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30318144
Proper citation: RRID:Addgene_122177 Copy
Species: Homo sapiens
Genetic Insert: RSL1D1
Vector Backbone Description: Backbone Size:5450; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29941930
Proper citation: RRID:Addgene_122301 Copy
Species: Homo sapiens
Genetic Insert: PHB
Vector Backbone Description: Backbone Size:7000; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31391345
Proper citation: RRID:Addgene_122231 Copy
Species: Homo sapiens
Genetic Insert: Prohibitin
Vector Backbone Description: Backbone Size:7700; Vector Backbone:pLX304; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31391345
Proper citation: RRID:Addgene_122230 Copy
Species: Homo sapiens
Genetic Insert: shRNA targeting PHB (see partial depositor seq)
Vector Backbone Description: Backbone Size:7000; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31391345
Proper citation: RRID:Addgene_122233 Copy
Species: Homo sapiens
Genetic Insert: RPS14
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:7000; Vector Backbone:MSCV hygro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29941930
Proper citation: RRID:Addgene_122236 Copy
Species: Homo sapiens
Genetic Insert: RSL1D1
Vector Backbone Description: Backbone Size:5170; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29941930
Proper citation: RRID:Addgene_122296 Copy
Species: Homo sapiens
Genetic Insert: KRASG12V
Vector Backbone Description: Backbone Size:6213; Vector Backbone:mycBioID-pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30194290
Proper citation: RRID:Addgene_122211 Copy
Species: Homo sapiens
Genetic Insert: RPS14 (partial)
Vector Backbone Description: Backbone Size:5450; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29941930
Proper citation: RRID:Addgene_122298 Copy
Species: Homo sapiens
Genetic Insert: HRASG12V
Vector Backbone Description: Backbone Size:6213; Vector Backbone:mycBioID-pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30194290
Proper citation: RRID:Addgene_122214 Copy
Species: Homo sapiens
Genetic Insert: KRASG12V
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3.1 myc BioID; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30194290
Proper citation: RRID:Addgene_122208 Copy
Species: Homo sapiens
Genetic Insert: NRASG12v
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3.1 myc BioID; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30194290
Proper citation: RRID:Addgene_122209 Copy
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