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Species: Synthetic
Genetic Insert: nptII promoter
Vector Backbone Description: Vector Backbone:pL0M-PU-pNeo; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30692983
Comments: pL0M-PU-pNeo. Backbone from Invitrogen for synthesised parts. Please visit https://www.biorxiv.org/content/early/2018/08/16/392423 for bioRxiv preprint.
Proper citation: RRID:Addgene_113978 Copy
Species: Synthetic
Genetic Insert: Level 1 golden gate cloning site
Vector Backbone Description: Vector Backbone:pLVC-P1-GG; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30692983
Comments: pLVC-P1-GG cloning cite Lv1. Backbone from Invitrogen for synthesised parts. Please visit https://www.biorxiv.org/content/early/2018/08/16/392423 for bioRxiv preprint.
Proper citation: RRID:Addgene_113979 Copy
Species: S. pyogenes
Genetic Insert: humanized S. pyogenes Cas9 nuclease
Vector Backbone Description: Vector Backbone:pUC ori vector; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24954249
Proper citation: RRID:Addgene_58778 Copy
Species: S. pyogenes
Genetic Insert: humanized S. pyogenes Cas9 nuclease
Vector Backbone Description: Vector Backbone:pUC ori vector; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24954249
Proper citation: RRID:Addgene_58780 Copy
Species: S. pyogenes
Genetic Insert: humanized S. pyogenes Cas9 nuclease
Vector Backbone Description: Vector Backbone:pUC ori vector; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24954249
Proper citation: RRID:Addgene_58783 Copy
Species: Synthetic
Genetic Insert: Four-fragment T7* RNA polymerase
Vector Backbone Description: Vector Backbone:pSB3C5; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:25080493
Proper citation: RRID:Addgene_59953 Copy
Species: Synthetic
Genetic Insert: K1FR sigma fragment
Vector Backbone Description: Vector Backbone:pSB3C5; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:25080493
Proper citation: RRID:Addgene_59956 Copy
Species: Synthetic
Genetic Insert: sfGFP
Vector Backbone Description: Vector Backbone:pSB3C5; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:25080493
Proper citation: RRID:Addgene_59960 Copy
Species: Synthetic
Genetic Insert: RVD sequence: NG NN HD NG
Vector Backbone Description: Vector Backbone:pFUS_B4; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:23734242
Comments: Plasmid was created using Golden Gate cloning.
Proper citation: RRID:Addgene_48599 Copy
Species: Synthetic
Genetic Insert: RVD sequence: NG NN NI HD
Vector Backbone Description: Vector Backbone:pFUS_B4; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:23734242
Comments: Plasmid was created using Golden Gate cloning.
Proper citation: RRID:Addgene_48593 Copy
Species: Synthetic
Genetic Insert: RVD sequence: NG NN NI NI
Vector Backbone Description: Vector Backbone:pFUS_B4; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:23734242
Comments: Plasmid was created using Golden Gate cloning.
Proper citation: RRID:Addgene_48592 Copy
Species: Synthetic
Genetic Insert: RVD sequence: NG HD NG NG
Vector Backbone Description: Vector Backbone:pFUS_B4; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:23734242
Comments: Plasmid was created using Golden Gate cloning.
Proper citation: RRID:Addgene_48591 Copy
Species: Synthetic
Genetic Insert: RVD sequence: NG NN HD HD
Vector Backbone Description: Vector Backbone:pFUS_B4; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:23734242
Comments: Plasmid was created using Golden Gate cloning.
Proper citation: RRID:Addgene_48597 Copy
Species: Synthetic
Genetic Insert: RVD sequence: NG NN HD NI
Vector Backbone Description: Vector Backbone:pFUS_B4; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:23734242
Comments: Plasmid was created using Golden Gate cloning.
Proper citation: RRID:Addgene_48596 Copy
Species: Synthetic
Genetic Insert: RVD sequence: NG NN NI NG
Vector Backbone Description: Vector Backbone:pFUS_B4; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:23734242
Comments: Plasmid was created using Golden Gate cloning.
Proper citation: RRID:Addgene_48595 Copy
Species: Synthetic
Genetic Insert: RVD sequence: NG NN NI NN
Vector Backbone Description: Vector Backbone:pFUS_B4; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:23734242
Comments: Plasmid was created using Golden Gate cloning.
Proper citation: RRID:Addgene_48594 Copy
Species: Synthetic
Genetic Insert: RVD sequence: NG HD NG HD
Vector Backbone Description: Vector Backbone:pFUS_B4; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:23734242
Comments: Plasmid was created using Golden Gate cloning.
Proper citation: RRID:Addgene_48589 Copy
Species: Synthetic
Genetic Insert: RVD sequence: NG NG NG NN
Vector Backbone Description: Vector Backbone:pFUS_B4; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:23734242
Comments: Plasmid was created using Golden Gate cloning.
Proper citation: RRID:Addgene_48622 Copy
Species: Synthetic
Genetic Insert: RVD sequence: NG NG NG NI
Vector Backbone Description: Vector Backbone:pFUS_B4; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:23734242
Comments: Plasmid was created using Golden Gate cloning.
Proper citation: RRID:Addgene_48620 Copy
Species: Synthetic
Genetic Insert: RVD sequence: NG HD HD HD
Vector Backbone Description: Vector Backbone:pFUS_B4; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:23734242
Comments: Plasmid was created using Golden Gate cloning.
Proper citation: RRID:Addgene_48581 Copy
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