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Species: Homo sapiens
Genetic Insert: Ratiometric pHLuorin2 fused to B4GALT1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4730; Vector Backbone:pRpHluorin-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35000377
Comments: Ratiometric pHLuorin2 sequence synthesized by Genscript, original sequence from https://www.scirp.org/journal/paperinformation.aspx?paperid=5043
Proper citation: RRID:Addgene_171719 Copy
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4730; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35000377
Comments: Ratiometric pHLuorin2 sequence synthesized by Genscript, original sequence from https://www.scirp.org/journal/paperinformation.aspx?paperid=5043
Proper citation: RRID:Addgene_171716 Copy
Species: Synthetic
Genetic Insert: C- and N termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
Vector Backbone Description: Vector Backbone:PINII; Vector Types:Multisite Gateway entry vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34211131
Comments: Multisite Gateway entry vector to express one hand of pTALECD-UGI. Use th Platinum Gate TALEN Kit (Kit # 1000000054 ) for assembling of TALE repeats, in which use this plasmid in the last step.
Proper citation: RRID:Addgene_171735 Copy
Species: Synthetic
Genetic Insert: C- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
Vector Backbone Description: Vector Backbone:PINII; Vector Types:Multisite Gateway entry vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34211131
Comments: Multisite Gateway entry vector to express one hand of pTALECD-UGI. Use th Platinum Gate TALEN Kit (Kit # 1000000053 ) for assembling of TALE repeats, in which use this plasmid in the last step.
Proper citation: RRID:Addgene_171734 Copy
Species: Homo sapiens
Genetic Insert: BEACON2
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32492431
Proper citation: RRID:Addgene_171698 Copy
Species: Homo sapiens
Genetic Insert: BEACON1
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32492431
Proper citation: RRID:Addgene_171697 Copy
Species: Synthetic
Genetic Insert: C- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
Vector Backbone Description: Vector Backbone:PINII; Vector Types:Multisite Gateway entry vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34211131
Comments: Multisite Gateway entry vector to express one hand of pTALECD-UGI. Use th Platinum Gate TALEN Kit (Kit # 1000000049 ) for assembling of TALE repeats, in which use this plasmid in the last step.
Proper citation: RRID:Addgene_171730 Copy
Species: Other
Genetic Insert: sgRNA scaffold-MS2-U6
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33972728
Comments: The tBE system with gRNA could be constructed by linearing pU6-ccdB-boxB-tBE-V5-mA3 (backbone vector of tBE system) and inserting the gRNA sequences amplificating from pUC57-sgRNA-MS2-U6. The detailed protocol could be found in Supplementary Protocol in the publication.
Proper citation: RRID:Addgene_171694 Copy
Species: S. pyogenes
Genetic Insert: nSpCas9
Vector Backbone Description: Vector Backbone:pEF1a; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33972728
Proper citation: RRID:Addgene_171691 Copy
Species: Mus musculus
Genetic Insert: mNeonGreen-FLAG-mgp100-T2A-PuroR
Vector Backbone Description: Backbone Marker:Based on plasmids published by Schmid-Burgk et al. 2016 (DOI: 10.1038/ncomms12338); Backbone Size:2542; Vector Backbone:pCRISPaint; Vector Types:Gene tagging; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32750334
Proper citation: RRID:Addgene_171802 Copy
Species: Branchiostoma lanceolatum, Streptomyces alboniger
Genetic Insert: mNeonGreen-T2A-PuroR
Vector Backbone Description: Backbone Marker:Based on plasmids published by Schmid-Burgk et al. 2016 (DOI: 10.1038/ncomms12338); Backbone Size:2516; Vector Backbone:pCRISPaint; Vector Types:Gene tagging; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32750334
Proper citation: RRID:Addgene_171800 Copy
Species: Synthetic
Genetic Insert: DogCatcher
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Backbone Size:3641; Vector Backbone:pDEST14; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34324879
Proper citation: RRID:Addgene_171772 Copy
Vector Backbone Description: Backbone Marker:Moon Lab; Backbone Size:0; Vector Backbone:pCMV pGL3 Luciferase; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Comments: Description: This is a home-cooked PGL3 Luciferase expression vector.
Reference: Ajamete Kaykas.
Proper citation: RRID:Addgene_17186 Copy
Species: Synthetic
Genetic Insert: RNA-based (p)ppGpp sensor
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5267; Vector Backbone:pETDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34487413
Comments: Addgene QC identified a C66Y change in the lacI coding sequence that may impact the leakiness of expression of the (p)ppGpp sensor and NirFP insert.
Proper citation: RRID:Addgene_171921 Copy
Species: Synthetic
Genetic Insert: mNeonGreen-F-Ova-T2A-BlastR
Vector Backbone Description: Backbone Marker:Based on plasmids published by Schmid-Burgk et al. 2016 (DOI: 10.1038/ncomms12338); Backbone Size:2577; Vector Backbone:pCRISPaint; Vector Types:Gene tagging; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32750334
Proper citation: RRID:Addgene_171808 Copy
Species: Synthetic
Genetic Insert: mNeonGreen-FLAG-gB-T2A-PuroR
Vector Backbone Description: Backbone Marker:Based on plasmids published by Schmid-Burgk et al. 2016 (DOI: 10.1038/ncomms12338); Backbone Size:2542; Vector Backbone:pCRISPaint; Vector Types:Gene tagging; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32750334
Proper citation: RRID:Addgene_171803 Copy
Species: Homo sapiens
Genetic Insert: Endophilin A1
Vector Backbone Description: Vector Backbone:mRuby2-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33947811
Proper citation: RRID:Addgene_171945 Copy
Species: Synthetic
Genetic Insert: mScarlet-F-gB-T2A-PuroR
Vector Backbone Description: Backbone Marker:Based on plasmids published by Schmid-Burgk et al. 2016 (DOI: 10.1038/ncomms12338); Backbone Size:2543; Vector Backbone:pCRISPaint; Vector Types:Gene tagging; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32750334
Proper citation: RRID:Addgene_171812 Copy
Species: Mus musculus
Genetic Insert: mScarlet-F-mgp100-T2A-PuroR
Vector Backbone Description: Backbone Marker:Based on plasmids published by Schmid-Burgk et al. 2016 (DOI: 10.1038/ncomms12338); Backbone Size:2543; Vector Backbone:pCRISPaint; Vector Types:Gene tagging; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32750334
Proper citation: RRID:Addgene_171811 Copy
Species: Homo sapiens
Genetic Insert: mScarlet-F-hgp100-T2A-PuroR
Vector Backbone Description: Backbone Marker:Based on plasmids published by Schmid-Burgk et al. 2016 (DOI: 10.1038/ncomms12338); Backbone Size:2543; Vector Backbone:pCRISPaint; Vector Types:Gene tagging; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32750334
Proper citation: RRID:Addgene_171810 Copy
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