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Species: Mus musculus
Genetic Insert: Pea3
Vector Backbone Description: Backbone Size:11600; Vector Backbone:RCASBP(A); Vector Types:Retroviral, avian expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15253939
Comments: Pea3 ETS domain fused to EnR.
Proper citation: RRID:Addgene_13909 Copy
Species: Mus musculus
Genetic Insert: Pea3
Vector Backbone Description: Backbone Size:11600; Vector Backbone:RCASBP(A); Vector Types:Retroviral, avian expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15253939
Comments: Full-length Pea3.
Proper citation: RRID:Addgene_13908 Copy
Species: Mus musculus
Genetic Insert: Id1
Vector Backbone Description: Backbone Marker:Sen Wu, Capecchi laboratory; Vector Backbone:pWS-TK6; Vector Types:Mammalian Expression, Mouse Targeting, Cre/Lox; Bacterial Resistance:Ampicillin
Comments: Linker between sfGFP and iCre-ERT2 is a mutated P2A sequence where 2 conserved amino acids are changed to Alanines. This mutated sequence does not skip.
The sfGFP fluorescence from the sfGFP-iCre-ERT2 is dim, as described in Nam and Capecchi, 2020 (https://doi.org/10.1186/s13064-020-00139-5) and a Figshare page (https://doi.org/10.6084/m9.figshare.13218437.v35). Thus, the sfGFP fluorescence should be detectable only in cells in which the marker gene expression level is high.
Homology arms subcloned by recombineering from a clone of RPCI-23 C57BL/6J mouse genomic DNA library.
Please make sure the plasmid DNA preparation is pure by gel electrophoresis before linearizing for an electroporation.
Proper citation: RRID:Addgene_139279 Copy
Species: Mus musculus
Genetic Insert: Mus musculus Akap1(1-31) M16L:MTOR(2021-2113):iRFP
Vector Backbone Description: Vector Backbone:piRFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32211893
Comments: The M16L mutation in the plasmid insert eliminates an alternative start codon that leads to an ER-targeted protein.
Proper citation: RRID:Addgene_139315 Copy
Species: Mus musculus
Genetic Insert: Id1
Vector Backbone Description: Backbone Marker:Sen Wu, Capecchi laboratory; Vector Backbone:pWS-TK6; Vector Types:Mammalian Expression, Mouse Targeting; Bacterial Resistance:Ampicillin
Comments: Homology arms subcloned by recombineering from a clone of RPCI-23 C57BL/6J mouse genomic DNA library.
Please make sure the plasmid DNA preparation is pure by gel electrophoresis before linearizing for an electroporation.
Please note there are a few ambiguous bases in Addgene’s NGS result. This is due to repetitive A-G region. Depositor confirms these bases do not affect plasmid function.
Proper citation: RRID:Addgene_139281 Copy
Species: Mus musculus
Genetic Insert: ISH probe for Olfr156 - UTR
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3000; Vector Backbone:pGEM-T-easy; Vector Types:ISH probe; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32209480
Proper citation: RRID:Addgene_139486 Copy
Species: Mus musculus
Genetic Insert: ER
Vector Backbone Description: Backbone Size:2618; Vector Backbone:pDONR-P2rP3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_139539 Copy
Species: Mus musculus
Genetic Insert: Cytoskeletal-linking protein 63
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4722; Vector Backbone:pmCherry-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18442980
Proper citation: RRID:Addgene_136293 Copy
Species: Mus musculus
Genetic Insert: PLEKHG6
Vector Backbone Description: Vector Backbone:pCDNA3.1 DEST_GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28692064
Proper citation: RRID:Addgene_136325 Copy
Species: Mus musculus
Genetic Insert: CCCNB2
Vector Backbone Description: Vector Backbone:pENTR_D-topo; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_136341 Copy
Species: Mus musculus
Genetic Insert: U6_sgRNA(Bcan)_U6_sgRNA(Ntrk1)
Vector Backbone Description: Backbone Marker:Addgene #50946; Vector Backbone:pKLV-U6gRNA-PGKpuro2ABFP; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29654229
Proper citation: RRID:Addgene_136413 Copy
Species: Mus musculus
Genetic Insert: src K295R Y527F
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pCMV5 *; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: * The depositing lab is not completely sure of the backbone for this plasmid; Addgene's sequencing results indicate that it may instead be pcDNA3. The plasmid should still function correctly, but users should consult Addgene's sequencing results and exercise caution if subcloning is necessary.
Proper citation: RRID:Addgene_13657 Copy
Species: Mus musculus
Genetic Insert: emiRFP703-myosin
Vector Backbone Description: Vector Backbone:pcDNA; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31932632
Proper citation: RRID:Addgene_136565 Copy
Species: Mus musculus
Genetic Insert: p53DD
Vector Backbone Description: Backbone Marker:Addgene #25752; Backbone Size:4422; Vector Backbone:pEN_TTmiRc2; Vector Types:Gateway Cloning; Bacterial Resistance:Gentamicin
Defining Citation: PMID:32291314
Proper citation: RRID:Addgene_136520 Copy
Species: Mus musculus
Genetic Insert: p53DD
Vector Backbone Description: Backbone Marker:Addgene #25736; Backbone Size:12944; Vector Backbone:pSLIK-Zeo; Vector Types:Mammalian Expression, Lentiviral, destinatioin vector for gateway cloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32291314
Proper citation: RRID:Addgene_136534 Copy
Species: Mus musculus
Genetic Insert: Oct4-P2A-Sox2-T2A-Klf4-E2A-cMyc
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pHAGE2; Vector Types:Bacterial Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31708402
Proper citation: RRID:Addgene_136541 Copy
Species: Mus musculus
Genetic Insert: Oct4-P2A-Sox2-T2A-Klf4
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pHAGE2; Vector Types:Bacterial Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31708402
Proper citation: RRID:Addgene_136552 Copy
Species: Mus musculus
Genetic Insert: Klf4-IRES-Sox2-E2A-cMyc
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pHAGE2; Vector Types:Bacterial Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31708402
Proper citation: RRID:Addgene_136553 Copy
Species: Mus musculus
Genetic Insert: Oct4-T2A-Klf4-E2A-cMyc
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pHAGE2; Vector Types:Bacterial Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31708402
Proper citation: RRID:Addgene_136554 Copy
Species: Mus musculus
Genetic Insert: src
Vector Backbone Description: Backbone Marker:BD Biosciences; Backbone Size:6600; Vector Backbone:pLNCX; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_13658 Copy
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