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Species: Synthetic
Genetic Insert: Cpf1 RVR variant
Vector Backbone Description: Backbone Size:4328; Vector Backbone:pUC ori vector; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28581492
Comments: This variant also recognizes TTTV and RATR PAMs (R = A or G).
Contains a U6-crRNA expression cassette (BbsI restriction sites for guide cloning).
For more information on Zhang lab CRISPR plasmids, please refer to www.addgene.org/crispr/zhang/.
Proper citation: RRID:Addgene_89354 Copy
Genetic Insert: GFP, Puromycin resistance
Vector Backbone Description: Vector Backbone:pMCB306, with addition of hU6 and BbsI sites for cloning of sgRNA; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28319085
Comments: pKHH030 is derived from pMCB306, which was originally derived from pU6-sgGFP-NT1 (addgene #46914) from the Weissman and Qi labs
Proper citation: RRID:Addgene_89358 Copy
Species: Homo sapiens
Genetic Insert: MS2-P65-HSF1_2A_Hygro
Vector Backbone Description: Vector Backbone:plenti; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28333914
Proper citation: RRID:Addgene_89308 Copy
Vector Backbone Description: Vector Backbone:pCAMBIA1300; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27212389
Comments: p35s::Cas9::RZ::gRNA::RZ
Proper citation: RRID:Addgene_89257 Copy
Species: Other
Genetic Insert: Channelrhodopsin
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4574; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27525487
Proper citation: RRID:Addgene_89256 Copy
Vector Backbone Description: Vector Backbone:pTX171; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27212389
Comments: pZmUbi1::Cas9::RZ cleavage site::gRNA::RZ cleavage site::RZ
Proper citation: RRID:Addgene_89259 Copy
Species: Homo sapiens
Genetic Insert: spastin
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4300; Vector Backbone:pCMV-Tag; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24478365
Comments: Cloning of spastin cDNA into pCMV-Tag EcoICR I and BamH I sites removed myc tag. To detect spastin use anti-spastin antibody.
Proper citation: RRID:Addgene_89322 Copy
Species: Homo sapiens
Genetic Insert: spastin
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4300; Vector Backbone:pCMV-Tag; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24478365
Comments: Cloning of spastin cDNA into pCMV-Tag EcoICR I and BamH I sites removed myc tag. To detect spastin use anti-spastin antibody.The endogenous M1 Kozak sequence was replaced with an optimal Kozak sequence to prevent leaky translation of M87 spastin.
Proper citation: RRID:Addgene_89321 Copy
Species: Homo sapiens
Genetic Insert: spastin
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4300; Vector Backbone:pCMV-Tag; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24478365
Comments: Cloning of spastin cDNA into pCMV-Tag EcoICR I and BamH I sites removed myc tag. To detect spastin use anti-spastin antibody.
Proper citation: RRID:Addgene_89320 Copy
Species: Homo sapiens
Genetic Insert: spastin
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4300; Vector Backbone:pCMV-Tag; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24478365
Comments: Cloning of spastin cDNA into pCMV-Tag EcoICR I and BamH I sites removed myc tag. To detect spastin use anti-spastin antibody.The endogenous M1 Kozak sequence was replaced with an optimal Kozak sequence to prevent leaky translation of M87 spastin.
Proper citation: RRID:Addgene_89319 Copy
Species: Synthetic
Genetic Insert: eGFP
Vector Backbone Description: Vector Backbone:pRFHUE; Vector Types:Binary plasmid for ATMT of filamentous fungi; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21663991
Comments: Binary plasmid for ATMT of filamentous fungi. The T-DNA contains a Hygromycin resistance gene under the control of A. nidulans trpC gen promoter and terminator
Proper citation: RRID:Addgene_89469 Copy
Species: Salmonella enterica
Genetic Insert: DeAct-SpvB
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3383; Vector Backbone:pTRE3G-luciferase; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28394337
Comments: Uses Clontech 3rd generation Tet-responsive promoter; also requires Tet-On 3G transactivator protein
Proper citation: RRID:Addgene_89455 Copy
Species: Homo sapiens
Genetic Insert: DeAct-GS1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3383; Vector Backbone:pTRE3G-luciferase; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28394337
Comments: Uses Clontech 3rd generation Tet-responsive promoter; also requires Tet-On 3G transactivator protein
Proper citation: RRID:Addgene_89454 Copy
Species: Homo sapiens
Genetic Insert: ezrin siRNA
Vector Backbone Description: Backbone Size:3300; Vector Backbone:pBS/U6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12769842
Comments: siRNA targeting ezrin. Sense strand of the hairpin is identical to a 22 nucleotide region in the target gene (ezrin [376-396]). Sequence is GGGTC CTACG CTGTG CAGGC CAAG.
Proper citation: RRID:Addgene_8945 Copy
Species: Mus musculus
Genetic Insert: PGC-1a
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1 myc-His A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14744933
Proper citation: RRID:Addgene_8943 Copy
Species: Synthetic
Genetic Insert: Asparaginyl endopeptidase from Oldenlandia affinis
Vector Backbone Description: Backbone Size:4540; Vector Backbone:pHYRSF53; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28369872
Proper citation: RRID:Addgene_89482 Copy
Vector Backbone Description: Vector Backbone:pMDC43; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:23056524
Comments: To enhance folding of the fusion proteins a flexible linker was introduced between the coding sequence of either fragment of YFP and the attR recombination site. For more information see: http://www.ibmcp.upv.es/FerrandoLabVectors/
Proper citation: RRID:Addgene_89487 Copy
Species: Caenorhabditis elegans
Genetic Insert: unc-122 promoter
Vector Backbone Description: Backbone Size:4300; Vector Backbone:MC10-RFP; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10545240
Comments: Marker for expression of RFP in coelomocytes. 4kb unc-122 promoter+ 4aa of 1st exon (8911-12903 of cosmid F11C3, start is at 12892) in SphI-BamHI sites of MC10-RFP (dsRed in AgeI-NotI).
Proper citation: RRID:Addgene_8938 Copy
Species: Caenorhabditis elegans
Genetic Insert: unc-122 promoter
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pPD95.77; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10545240
Comments: Marker for expression of GFP in coelomocytes. 4kb unc-122 promoter+ 4aa of 1st exon (8911-12903 of cosmid F11C3, start is at 12892) in Pst-BamHI sites of pPD95.77.
Proper citation: RRID:Addgene_8937 Copy
Genetic Insert: pMarC9-R6k
Vector Backbone Description: Backbone Marker:Victor de Lorenzo lab; Vector Backbone:pBAM1; Vector Types:; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:30962569
Comments: BW29427 is an E. coli strain with diaminopimelic acid (DAP) auxotrophy. BW29427 growth requires 300uM DAP even when cultured in LB. Importantly, BW29427 does not grow in the absence of DAP, which simplifies counterselection of this host strain following biparental mating with Vibrio natriegens.
Please visit https://www.biorxiv.org/content/early/2016/06/12/058487 for bioRxiv preprint.
Proper citation: RRID:Addgene_89477 Copy
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