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Species: Homo sapiens
Genetic Insert: SRSF1 cDNA
Vector Backbone Description: Vector Backbone:pcDNA5-3xFLAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38917794
Proper citation: RRID:Addgene_218974 Copy
Species: Homo sapiens
Genetic Insert: hFGF1
Vector Backbone Description: Backbone Marker:GeneScript; Backbone Size:3647; Vector Backbone:pG12; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38647395
Comments: To make pAAV-G12-hFGF1, hFGF1 was amplified from pDONR223_FGF1_WT, a gift from Jesse Boehm, William Hahn, and David Root (Addgene plasmid #81825). Subsequently, the amplified hFGF1 was swapped with AkaLuc in pAAV-G12-AkaLuc using AgeI and EcoRI restriction enzymes.
Proper citation: RRID:Addgene_219554 Copy
Species: Homo sapiens
Genetic Insert: The heat shock protein 18.2 (HSP) terminator
Vector Backbone Description: Backbone Size:2251; Vector Backbone:Level 0 - 3U + terminator - like; Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:36499768
Proper citation: RRID:Addgene_219740 Copy
Species: Homo sapiens
Genetic Insert: gRNA-com-3'end (S. pyogenes Cas9)
Vector Backbone Description: Vector Backbone:pU6; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38773305
Comments: QTC223
Proper citation: RRID:Addgene_219947 Copy
Species: Homo sapiens
Genetic Insert: CMV-EGFP(A111V/L202S); U6-A111V-gRNA-com-3'end; U6-L202S-gRNA-MS2-SL3
Vector Backbone Description: Vector Backbone:pDG461 ; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38773305
Comments: QTC312
Proper citation: RRID:Addgene_219946 Copy
Species: Homo sapiens
Genetic Insert: CMV-EGFP(A111V/L202S); U6-A111V-gRNA-com-3'end; U6-L202S-gRNA-MS2-3'end
Vector Backbone Description: Vector Backbone:pDG461 ; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38773305
Comments: QTC311
Proper citation: RRID:Addgene_219945 Copy
Species: Homo sapiens
Genetic Insert: apt-CBE-SL3_PT2A_apt-ABE8.20
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38773305
Comments: QTC284
Proper citation: RRID:Addgene_219944 Copy
Species: Homo sapiens
Genetic Insert: unc-104 promoter::human KIF1Bß(Q98L)
Vector Backbone Description: Vector Backbone:∆pSM; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_219961 Copy
Species: Homo sapiens
Genetic Insert: unc-104promoter::KIF1Bß (1770 a.a. isoform)
Vector Backbone Description: Vector Backbone:∆pSM; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_219960 Copy
Species: Homo sapiens
Genetic Insert: betaglobin-5'UTR-NanoLuc
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_219989 Copy
Species: Homo sapiens
Genetic Insert: DDX3X(D354V)
Vector Backbone Description: Vector Backbone:pOCC120; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Comments: MBP- and His-tag can be cleaved with 3C-Protease
Proper citation: RRID:Addgene_219986 Copy
Species: Homo sapiens
Genetic Insert: DDX3X(WT)
Vector Backbone Description: Vector Backbone:pOCC120; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Comments: MBP- and His-tag can be cleaved with 3C-Protease
Proper citation: RRID:Addgene_219985 Copy
Species: Homo sapiens
Genetic Insert: Sp-nCas9(D10A)-SpRY_P2A-mCherry
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38773305
Comments: QTC290; could also be used for dual-nicking or other editing modalities
Proper citation: RRID:Addgene_219935 Copy
Species: Homo sapiens
Genetic Insert: Sp-nCas9(D10A)-NG_P2A-mCherry
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38773305
Comments: QTC24; could also be used for dual-nicking or other editing modalities
Proper citation: RRID:Addgene_219934 Copy
Species: Homo sapiens
Genetic Insert: von Hippel-Lindau tumor suppressor
Vector Backbone Description: Backbone Size:8400; Vector Backbone:pLEX_307 (Plasmid #41392); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38514884
Proper citation: RRID:Addgene_220147 Copy
Species: Homo sapiens
Genetic Insert: Bcl2
Vector Backbone Description: Backbone Size:4702; Vector Backbone:pEMD-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_220301 Copy
Species: Homo sapiens
Genetic Insert: von Hippel-Lindau tumor suppressor
Vector Backbone Description: Backbone Size:8400; Vector Backbone:pLEX_307 (Plasmid #41392); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38514884
Proper citation: RRID:Addgene_220149 Copy
Species: Homo sapiens
Genetic Insert: Bcl2
Vector Backbone Description: Backbone Size:4702; Vector Backbone:pEMD-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_220304 Copy
Species: Homo sapiens
Genetic Insert: Bcl2
Vector Backbone Description: Backbone Size:4702; Vector Backbone:pEMD-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_220300 Copy
Species: Homo sapiens
Genetic Insert: TEX264
Vector Backbone Description: Backbone Marker:Thermofisher; Backbone Size:5413; Vector Backbone:pCDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32152270
Proper citation: RRID:Addgene_220204 Copy
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