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Species: Homo sapiens
Genetic Insert: actin beta
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pENTR-TOPO; Vector Types:Donor-plasmid; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30890647
Comments: Please visit https://www.biorxiv.org/content/10.1101/445973v1 for BioRxiv preprint
Proper citation: RRID:Addgene_118383 Copy
Species: Homo sapiens
Genetic Insert: actin beta
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pENTR-TOPO; Vector Types:Donor-plasmid; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30890647
Comments: Please visit https://www.biorxiv.org/content/10.1101/445973v1 for BioRxiv preprint
Proper citation: RRID:Addgene_118380 Copy
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pDEST27; Vector Types:Mammalian Expression, Destination; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:30890647
Comments: Please visit https://www.biorxiv.org/content/10.1101/445973v1 for BioRxiv preprint
Proper citation: RRID:Addgene_118368 Copy
Species: Other
Genetic Insert: MvuTFIIB mini intein
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pRSFDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29055778
Proper citation: RRID:Addgene_118401 Copy
Species: Other
Genetic Insert: MvuTFIIB mini intein
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pRSFDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29055778
Proper citation: RRID:Addgene_118400 Copy
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pDEST27; Vector Types:Mammalian Expression, Destination; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:30890647
Comments: Please visit https://www.biorxiv.org/content/10.1101/445973v1 for BioRxiv preprint
Proper citation: RRID:Addgene_118369 Copy
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pDEST27; Vector Types:Mammalian Expression, Destination; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:30890647
Comments: Please visit https://www.biorxiv.org/content/10.1101/445973v1 for BioRxiv preprint
Proper citation: RRID:Addgene_118366 Copy
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pDEST27; Vector Types:Mammalian Expression, Destination; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:30890647
Comments: Please visit https://www.biorxiv.org/content/10.1101/445973v1 for BioRxiv preprint
Proper citation: RRID:Addgene_118371 Copy
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pDEST27; Vector Types:Mammalian Expression, Destination; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:30890647
Comments: Please visit https://www.biorxiv.org/content/10.1101/445973v1 for BioRxiv preprint
Proper citation: RRID:Addgene_118372 Copy
Species: Homo sapiens
Genetic Insert: hHSF4b
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16371476
Comments: Transcript Variant: This variant (2) uses two alternate in-frame splice sites in the 3' coding region, compared to variant 1, resulting in a longer protein (isoform b). This variant, alternatively referred to as HSF4b, functions as a transcriptional activator of the constitutive expression of heat shock genes.
Proper citation: RRID:Addgene_118357 Copy
Species: Homo sapiens
Genetic Insert: hHSF4b
Vector Backbone Description: Backbone Marker:Sadowski and Ptashne; Backbone Size:3396; Vector Backbone:pSG424; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16371476
Comments: Transcript Variant: This variant (2) uses two alternate in-frame splice sites in the 3' coding region, compared to variant 1, resulting in a longer protein (isoform b). This variant, alternatively referred to as HSF4b, functions as a transcriptional activator of the constitutive expression of heat shock genes.
Proper citation: RRID:Addgene_118359 Copy
Species: Homo sapiens
Genetic Insert: hHSF4b
Vector Backbone Description: Backbone Marker:Sadowski and Ptashne; Backbone Size:3396; Vector Backbone:pSG424; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16371476
Comments: Transcript Variant: This variant (2) uses two alternate in-frame splice sites in the 3' coding region, compared to variant 1, resulting in a longer protein (isoform b). This variant, alternatively referred to as HSF4b, functions as a transcriptional activator of the constitutive expression of heat shock genes.
Proper citation: RRID:Addgene_118358 Copy
Species: Homo sapiens
Genetic Insert: hSUMO1
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4076; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12665592
Comments: Addgene NGS results found S2A within the SUMO1 translation compared to the closest NCBI reference (NP_003343.1). The depositing laboratory affirms that to their knowledge, this mutation does not change protein function
Proper citation: RRID:Addgene_118361 Copy
Species: Homo sapiens
Genetic Insert: hHSF2 shRNA
Vector Backbone Description: Backbone Marker:Oligoengine; Backbone Size:3176; Vector Backbone:pSUPER; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17213196
Proper citation: RRID:Addgene_118346 Copy
Species: Mycobacterium tuberculosis
Genetic Insert: MscL
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5856; Vector Backbone:pET19b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30275500
Proper citation: RRID:Addgene_118342 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SPE1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3716; Vector Backbone:pET-20b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: For over expression experiments please use Escherichia coli BL21 or similar strains
Proper citation: RRID:Addgene_118343 Copy
Species: Homo sapiens
Genetic Insert: hHSF1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5522; Vector Backbone:pcDNA3.1/myc-His(-) A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19229036
Proper citation: RRID:Addgene_118350 Copy
Species: Synthetic
Genetic Insert: ytdTomato
Vector Backbone Description: Vector Backbone:pDRF1-GW; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30783202
Proper citation: RRID:Addgene_118456 Copy
Species: Synthetic
Genetic Insert: ytdtomato-T2A-mTurquoise2
Vector Backbone Description: Vector Backbone:pDRF1-GW; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30783202
Proper citation: RRID:Addgene_118451 Copy
Species: Synthetic
Genetic Insert: ymVenus
Vector Backbone Description: Vector Backbone:pFA6a; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30783202
Comments: Please note the absence of a linker region at the 5’ end of the FP. Using a FW primer upstream of the FP will introduce a stopcodon. Please use FW primers annealing on the FP itself.
Proper citation: RRID:Addgene_118459 Copy
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