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Species: Homo sapiens
Genetic Insert: human SENP6 catalytic domain
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17591783
Proper citation: RRID:Addgene_16359 Copy
Species: Homo sapiens
Genetic Insert: human SENP2 catalytic domain
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17591783
Proper citation: RRID:Addgene_16357 Copy
Species: Homo sapiens
Genetic Insert: human SENP1 catalytic domain
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17591783
Proper citation: RRID:Addgene_16356 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Dcp2
Vector Backbone Description: Backbone Size:5827; Vector Backbone:pRP1903; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32553117
Comments: Impaired Edc3 binding sites (first HLM, and 5 additional HLMs in C-terminal domain).
5' sequencing primers:
Dcp2-seq1b:CGAATTTTACGTCTGAGGAAAG. Dcp2-seq2b: gacatagattgttgcattag. Dcp2-seq3b: cttcagcatttgaaagagca Dcp2-seq4b:gcaaaacaataatgatgaaa Dcp2-seq5b: gagaacaatttccaagacgg. Dcp2-seq7:tgaaacgcaacgacgctaca Dcp2-seq8b: GATACCCAGATCATATGAAACG
Proper citation: RRID:Addgene_163582 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Dcp2
Vector Backbone Description: Backbone Size:5827; Vector Backbone:pRP1903; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32553117
Comments: C-terminal domain (327-970) containing 5 impaired Edc3 binding sites (5 additional HLMs in C-terminal domain); N-terminal domain (1-299) including first HLM is removed.
5' sequencing primers:
Dcp2-seq1b:CGAATTTTACGTCTGAGGAAAG. Dcp2-seq2b: gacatagattgttgcattag. Dcp2-seq3b: cttcagcatttgaaagagca Dcp2-seq4b:gcaaaacaataatgatgaaa Dcp2-seq5b: gagaacaatttccaagacgg. Dcp2-seq7:tgaaacgcaacgacgctaca Dcp2-seq8b: GATACCCAGATCATATGAAACG
Proper citation: RRID:Addgene_163581 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Dcp2
Vector Backbone Description: Backbone Size:5827; Vector Backbone:pRP1903; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32553117
Comments: 5' sequencing primers: Dcp2-seq1b:CGAATTTTACGTCTGAGGAAAG. Dcp2-seq2b: gacatagattgttgcattag. Dcp2-seq3b: cttcagcatttgaaagagca Dcp2-seq4b:gcaaaacaataatgatgaaa Dcp2-seq5b: gagaacaatttccaagacgg. Dcp2-seq7:tgaaacgcaacgacgctaca Dcp2-seq8b: GATACCCAGATCATATGAAACG
Proper citation: RRID:Addgene_163585 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Dcp2
Vector Backbone Description: Backbone Size:5827; Vector Backbone:pRP1903; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32553117
Comments: Impaired Edc3 binding site (first HLM); impaired RNA cap recognition site (W50, D54).
5' sequencing primers:
Dcp2-seq1b:CGAATTTTACGTCTGAGGAAAG. Dcp2-seq2b: gacatagattgttgcattag. Dcp2-seq3b: cttcagcatttgaaagagca Dcp2-seq4b:gcaaaacaataatgatgaaa Dcp2-seq5b: gagaacaatttccaagacgg. Dcp2-seq7:tgaaacgcaacgacgctaca Dcp2-seq8b: GATACCCAGATCATATGAAACG
Proper citation: RRID:Addgene_163580 Copy
Species: Mus musculus
Genetic Insert: mouse NL2
Vector Backbone Description: Vector Backbone:pGEX4T3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31775031
Proper citation: RRID:Addgene_163623 Copy
Species: Homo sapiens
Genetic Insert: DCLK1
Vector Backbone Description: Vector Backbone:pLenti6; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32755567
Comments: Please note that the Addgene NGS sequence differs from the depositor's annotated sequence. These differences do not affect plasmid function.
Proper citation: RRID:Addgene_163625 Copy
Species: Homo sapiens
Genetic Insert: DCLK1
Vector Backbone Description: Vector Backbone:pLenti6; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32755567
Comments: Please note that the Addgene NGS sequence differs from the depositor's annotated sequence. These differences do not affect plasmid function.
Proper citation: RRID:Addgene_163626 Copy
Species: Synthetic
Genetic Insert: CsChrimson.mVenus
Vector Backbone Description: Backbone Marker:synthetic- Potter Lab; Backbone Size:8335; Vector Backbone:p10QUAS; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35442190
Comments: Read the preprint on bioRxiv: https://www.biorxiv.org/content/10.1101/2020.11.07.355651v2
Also refer to: Klapoetke, N.C., Murata, Y., Kim, S.S., Pulver, S.R., Birdsey-Benson, A., Cho, Y.K., Morimoto, T.K., Chuong, A.S., Carpenter, E.J., Tian, Z., et al. (2014). Independent optical excitation of distinct neural populations. Nat Methods 11, 338-346. PMID: 24509633, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3943671/
Proper citation: RRID:Addgene_163629 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Dcp2
Vector Backbone Description: Backbone Size:5827; Vector Backbone:pRP1903; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32553117
Comments: 5' sequencing primers: Dcp2-seq1b:CGAATTTTACGTCTGAGGAAAG. Dcp2-seq2b: gacatagattgttgcattag. Dcp2-seq3b: cttcagcatttgaaagagca Dcp2-seq4b:gcaaaacaataatgatgaaa Dcp2-seq5b: gagaacaatttccaagacgg. Dcp2-seq7:tgaaacgcaacgacgctaca Dcp2-seq8b: GATACCCAGATCATATGAAACG
Proper citation: RRID:Addgene_163574 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Dcp2
Vector Backbone Description: Backbone Size:5827; Vector Backbone:pRP1903; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32553117
Comments: Lacking C-terminal domain.
5' sequencing primers:
Dcp2-seq1b:CGAATTTTACGTCTGAGGAAAG. Dcp2-seq2b: gacatagattgttgcattag. Dcp2-seq3b: cttcagcatttgaaagagca Dcp2-seq4b:gcaaaacaataatgatgaaa Dcp2-seq5b: gagaacaatttccaagacgg. Dcp2-seq7:tgaaacgcaacgacgctaca Dcp2-seq8b: GATACCCAGATCATATGAAACG
Proper citation: RRID:Addgene_163577 Copy
Species: Other
Genetic Insert: GaLV MTR envelope
Vector Backbone Description: Backbone Marker:Addgene 15802; Backbone Size:4764; Vector Backbone:phCMV-ECO-ENV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31586074
Comments: This plasmid was generated by modification of Addgene plasmid 15802 to excise ENV-Eco and replace with ENV GALV-MTR.
Proper citation: RRID:Addgene_163612 Copy
Species: Homo sapiens
Genetic Insert: BCL2
Vector Backbone Description: Backbone Size:6574; Vector Backbone:MSCV_IRES_huDC2; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31586074
Comments: The huCD2 portion of the MSCV_IRES_huDC2 backbone was added by Dr Martin Turner, Babraham Institute, Cambridge by modification of Addgene plasmid # 27490.
Proper citation: RRID:Addgene_163611 Copy
Species: Homo sapiens
Genetic Insert: ODC
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5246; Vector Backbone:pET-28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33514717
Proper citation: RRID:Addgene_163614 Copy
Species: Synthetic
Genetic Insert: OAZ-GSY-SPM
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5246; Vector Backbone:pET-28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33514717
Proper citation: RRID:Addgene_163613 Copy
Species: Homo sapiens
Genetic Insert: TGFa-GSY-SPM
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5209; Vector Backbone:pET-28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33514717
Proper citation: RRID:Addgene_163615 Copy
Species: Synthetic
Genetic Insert: DHB:2xmKate2::3xHA
Vector Backbone Description: Backbone Marker:Bob Goldstein/Ari Pani; Backbone Size:11926; Vector Backbone:pAP088; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33350383
Comments: 2xmKate2 fusion of C. elegans codon-optimized DNA Helicase B (DHB) under the rps-27 promoter for ubiquitous expression. Plasmid is for CRISPR/Cas9-mediated single copy insertion at the ttTi4348 site on Chromosome I and can be paired with sgRNA plasmid pAP082
Proper citation: RRID:Addgene_163641 Copy
Species: Homo sapiens
Genetic Insert: mannosidase alpha class 2A member 1
Vector Backbone Description: Backbone Marker:Dr. Lu Lei's lab; Backbone Size:5523; Vector Backbone:pDmyc-neo-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34533190
Proper citation: RRID:Addgene_163648 Copy
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