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Species: Synthetic
Genetic Insert: mScarlet-I
Vector Backbone Description: Vector Backbone:pFru97; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30631309
Comments: Please visit https://www.biorxiv.org/content/early/2018/09/07/402172 for bioRxiv preprint.
Proper citation: RRID:Addgene_118497 Copy
Species: Synthetic
Genetic Insert: mCardinal
Vector Backbone Description: Vector Backbone:pAG408; Vector Types:Bacterial Expression, miniTn5 delivery vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30631309
Comments: Please visit https://www.biorxiv.org/content/early/2018/09/07/402172 for bioRxiv preprint.
Proper citation: RRID:Addgene_118522 Copy
Species: Synthetic
Genetic Insert: mScarlet-I
Vector Backbone Description: Vector Backbone:pAG408; Vector Types:Bacterial Expression, miniTn5 delivery vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30631309
Comments: Please visit https://www.biorxiv.org/content/early/2018/09/07/402172 for bioRxiv preprint.
Proper citation: RRID:Addgene_118521 Copy
Species: Synthetic
Genetic Insert: TagBFP2
Vector Backbone Description: Vector Backbone:pAG408; Vector Types:Bacterial Expression, miniTn5 delivery vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30631309
Comments: Please visit https://www.biorxiv.org/content/early/2018/09/07/402172 for bioRxiv preprint.
Proper citation: RRID:Addgene_118524 Copy
Species: Synthetic
Genetic Insert: mTurquoise2
Vector Backbone Description: Vector Backbone:pFru97; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30631309
Comments: Please visit https://www.biorxiv.org/content/early/2018/09/07/402172 for bioRxiv preprint.
Proper citation: RRID:Addgene_118485 Copy
Species: Synthetic
Genetic Insert: sYFP2
Vector Backbone Description: Vector Backbone:pFru97; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30631309
Comments: Please visit https://www.biorxiv.org/content/early/2018/09/07/402172 for bioRxiv preprint.
Proper citation: RRID:Addgene_118487 Copy
Species: Synthetic
Genetic Insert: mTurquoise2
Vector Backbone Description: Vector Backbone:pAG408; Vector Types:Bacterial Expression, miniTn5 delivery vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30631309
Comments: Please visit https://www.biorxiv.org/content/early/2018/09/07/402172 for bioRxiv preprint.
Proper citation: RRID:Addgene_118525 Copy
Species: Synthetic
Genetic Insert: mOrange2
Vector Backbone Description: Vector Backbone:pAG408; Vector Types:Bacterial Expression, miniTn5 delivery vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30631309
Comments: Please visit https://www.biorxiv.org/content/early/2018/09/07/402172 for bioRxiv preprint.
Proper citation: RRID:Addgene_118528 Copy
Species: Synthetic
Genetic Insert: TagBFP2
Vector Backbone Description: Vector Backbone:pFru97; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30631309
Comments: Please visit https://www.biorxiv.org/content/early/2018/09/07/402172 for bioRxiv preprint.
Proper citation: RRID:Addgene_118492 Copy
Species: Synthetic
Genetic Insert: mClover3
Vector Backbone Description: Vector Backbone:pFru97; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30631309
Comments: Please visit https://www.biorxiv.org/content/early/2018/09/07/402172 for bioRxiv preprint.
Proper citation: RRID:Addgene_118491 Copy
Species: Synthetic
Genetic Insert: sGFP2
Vector Backbone Description: Vector Backbone:pFru97; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30631309
Comments: Please visit https://www.biorxiv.org/content/early/2018/09/07/402172 for bioRxiv preprint.
Proper citation: RRID:Addgene_118494 Copy
Species: Synthetic
Genetic Insert: mCardinal
Vector Backbone Description: Vector Backbone:pFru97; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30631309
Comments: Please visit https://www.biorxiv.org/content/early/2018/09/07/402172 for bioRxiv preprint.
Proper citation: RRID:Addgene_118490 Copy
Species: Synthetic
Genetic Insert: sGFP2
Vector Backbone Description: Vector Backbone:pFru97; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30631309
Comments: Please visit https://www.biorxiv.org/content/early/2018/09/07/402172 for bioRxiv preprint.
Proper citation: RRID:Addgene_118510 Copy
Species: Synthetic
Genetic Insert: mScarlet-I
Vector Backbone Description: Vector Backbone:pFru97; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30631309
Comments: Please visit https://www.biorxiv.org/content/early/2018/09/07/402172 for bioRxiv preprint.
Proper citation: RRID:Addgene_118513 Copy
Species: Danio rerio
Genetic Insert: ZebrafishCommunity-bsx-Right
Vector Backbone Description: Backbone Marker:Addgene plasmid #32287; Backbone Size:6447; Vector Backbone:JDS71; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21822241
Comments: Target binding site: TATCTCTGGATCTCGAAC
It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.3kb and 5.8kb.
Proper citation: RRID:Addgene_41209 Copy
Species: Danio rerio
Genetic Insert: ZebrafishCommunity-bmp10-Left
Vector Backbone Description: Backbone Marker:Addgene plasmid #32287; Backbone Size:6447; Vector Backbone:JDS71; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21822241
Comments: Target binding site: TCAGCTCCCCGGAGAGGC
It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.3kb and 5.8kb.
Proper citation: RRID:Addgene_41206 Copy
Species: Danio rerio
Genetic Insert: ZebrafishCommunity-bbc3-(puma)-Right
Vector Backbone Description: Backbone Marker:Addgene plasmid #32288; Backbone Size:6447; Vector Backbone:JDS74; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21822241
Comments: Target binding site: TGGCCAGGCGTCCTGACG
It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.3kb and 5.8kb.
Proper citation: RRID:Addgene_41205 Copy
Species: Danio rerio
Genetic Insert: ZebrafishCommunity-avpr1b-Right
Vector Backbone Description: Backbone Marker:Addgene plasmid #32287; Backbone Size:6447; Vector Backbone:JDS71; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21822241
Comments: Target binding site: TTGGCCACATCTTCGTTC
It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.3kb and 5.8kb.
Proper citation: RRID:Addgene_41203 Copy
Species: Homo sapiens
Genetic Insert: Plk1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5527; Vector Backbone:pRc/CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7962193
Comments: cDNA fragments spanning parts of the catalytic domains of human protein kinases were amplified by PCR, as described by Schultz and Nigg (1993). A 144 bp fragment (named HsPK28) showing 88% nucleotide identity to Drosophila polo was used to probe a λgt 10 library prepared from a human nasopharyngeal carcinoma (Hitt et al., 1989). Approximately 500,000 plaques were screened by plaque hybridization (Sambrook et al., 1989), and 17 phage showing strong hybridization were purified. DNA was prepared using Lambdasorb (Promega Biotech, Madison, WI).
One plasmid (referred to as Plk1-pGEM) with a 2143 bp insert contained the entire coding sequence for a protein kinase closely related to Drosophila polo. This insert was sequenced on both strands by the method of Chen and Seeburg (1985), using the Sequenase kit (United States Biochemicals).
To express a full-length Plk1 protein, the 1998 bp fragment of Plk1-pGEM was introduced into the pRc/CMV vector (Invitrogen Corporation, San Diego), to create Plk1-CMV. An N-terminally myc-tagged Plk1-CMV was also constructed. In the resulting mycplk1 protein, the N terminus of Plk1 is extended by the oligopeptide MEQKLISEEDLNMNSCSPGS (Evan et al., 1985).
Proper citation: RRID:Addgene_41160 Copy
Species: Danio rerio
Genetic Insert: ZebrafishCommunity-avpr1b-Left
Vector Backbone Description: Backbone Marker:Addgene plasmid #32285; Backbone Size:6447; Vector Backbone:JDS70; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21822241
Comments: Target binding site: TCACGGCGAACAACACTA
It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.3kb and 5.8kb.
Proper citation: RRID:Addgene_41202 Copy
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