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Species: Homo sapiens
Genetic Insert: Pin1
Vector Backbone Description: Backbone Size:5200; Vector Backbone:pMCSG7; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_40773 Copy
Species: Homo sapiens
Genetic Insert: DUSP6
Vector Backbone Description: Backbone Size:5200; Vector Backbone:pMCSG7; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24704509
Proper citation: RRID:Addgene_40772 Copy
Species: Mus musculus
Genetic Insert: Ctcf
Vector Backbone Description: Backbone Size:8339; Vector Backbone:FUW-TetO; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22980981
Proper citation: RRID:Addgene_40801 Copy
Genetic Insert: 6 copies of the CXCR4 small RNA target site
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:6273; Vector Backbone:psiCheck2; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21878547
Comments: This plasmid also contains a firefly reporter cassette that has been specifically designed to be an intraplasmid transfection normalization reporter.
Proper citation: RRID:Addgene_40763 Copy
Species: Homo sapiens
Genetic Insert: MAP2K1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:10200; Vector Backbone:pCEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8052857
Proper citation: RRID:Addgene_40809 Copy
Species: Homo sapiens
Genetic Insert: MAP2K1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:10200; Vector Backbone:pCEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8052857
Proper citation: RRID:Addgene_40808 Copy
Species: Homo sapiens
Genetic Insert: MAP2K2
Vector Backbone Description: Backbone Size:5300; Vector Backbone:pMM9 from pMB1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9121442
Proper citation: RRID:Addgene_40806 Copy
Species: Synthetic
Genetic Insert: mRFP
Vector Backbone Description: Backbone Size:6336; Vector Backbone:PBCAG-eGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22521325
Proper citation: RRID:Addgene_40996 Copy
Species: Mus musculus
Genetic Insert: Arl13b
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7100; Vector Backbone:pcDNA-DEST40; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21976698
Comments: In Larkins et al (2011), this is plasmid "Arl13b untagged" as referenced in Figure 2. Full-length Arl13b with stop codon (pENTR-Arl13b1) was Gateway cloned into pcDNA-DEST40. Because of the stop codon, the backbone-encoded C-terminal tags are not expressed.
Proper citation: RRID:Addgene_40874 Copy
Species: Mus musculus
Genetic Insert: Arl13b
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7780; Vector Backbone:pcDNA-DEST47; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21976698
Comments: In Larkins et al (2011), this is plasmid "Arl13b GFP tagged" as referenced in Figure 2. Full-length Arl13b without a stop codon (pENTR-Arl13b2) was Gateway cloned into pcDNA-DEST47.
Proper citation: RRID:Addgene_40872 Copy
Species: Homo sapiens
Genetic Insert: ERBB2
Vector Backbone Description: Backbone Size:5200; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22908275
Proper citation: RRID:Addgene_40987 Copy
Species: Homo sapiens
Genetic Insert: ERBB2
Vector Backbone Description: Backbone Size:5200; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22908275
Proper citation: RRID:Addgene_40982 Copy
Species: Homo sapiens
Genetic Insert: ERBB2
Vector Backbone Description: Backbone Size:5200; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22908275
Proper citation: RRID:Addgene_40978 Copy
Species: Synthetic
Genetic Insert: PiggyBac transposon 3' terminal repeats
Vector Backbone Description: Backbone Size:5551; Vector Backbone:pCAGGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22521325
Proper citation: RRID:Addgene_40973 Copy
Vector Backbone Description: Backbone Marker:Addgene plasmid 31829; Vector Backbone:pminiTol2; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: The plasmid contains Tol2 transposon.
Proper citation: RRID:Addgene_40970 Copy
Species: Mus musculus
Genetic Insert: Ellis van Creveld syndrome 2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7500; Vector Backbone:pEF5/FRT/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22981989
Proper citation: RRID:Addgene_41008 Copy
Species: Mus musculus
Genetic Insert: p160 myb binding protein
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1 myc-His A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14744933
Comments: There is a K935R mutation in this insert. The mutation is not known to affect p160MBP function.
Proper citation: RRID:Addgene_41 Copy
Species: E. coli
Genetic Insert: GFP-LacI**
Vector Backbone Description: Backbone Marker:Andrew W. Murray Lab, UCSF (Straight, Sedat, & Murray, 1998. PMID: 9813090); Backbone Size:4600; Vector Backbone:pAFS135; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21724830
Comments: The plasmid pAFS135 (Straight, Sedat, and Murray, 1998) contains GFP(S65T, V163A)-LacI(P3Y, del C-terminal 11 aa)-SV40 NLS under the control of a HIS3 promoter fragment. To construct pAT123, the HIS3 marker of AFS135 was replaced with a ScaI-DraIII fragment from RS405 containing LEU2 (a kind gift from Karine Dubrana and Susan Gasser).
The plasmid pAT222 (Addgene plasmid #40942) was obtained by site-directed mutagenesis of pAT123 to create GFP-LacI**, using the primers (base mutations in lowercase):
5’- GTGGCACAgCAACTGGCGGaCAAAggtggcggaTCGTTGCTGATTGGCGTTGCCtCCT-3’ and
5’- AGGaGGCAACGCCAATCAGCAACGAtccgccaccTTTGtCCGCCAGTTGcTGTGCCAC- 3’.
The LacI** variant was then subcloned into pAFS135 using NotI and XhoI restriction sites to create plasmid pAT253.
Proper citation: RRID:Addgene_40943 Copy
Genetic Insert: PM-FRB-mRFP-T2A-FKBP-5-ptase
Vector Backbone Description: Vector Backbone:pmRFP-C1; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22357943
Proper citation: RRID:Addgene_40896 Copy
Species: Homo sapiens
Genetic Insert: shKIBRA-B
Vector Backbone Description: Backbone Marker:Addgene 8453; Backbone Size:7032; Vector Backbone:pLKO.1 puro; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22614006
Proper citation: RRID:Addgene_40889 Copy
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