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Species: Synthetic
Genetic Insert: ssAAV-Ple304-iCre
Vector Backbone Description: Vector Backbone:AAV2; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30062914
Comments: Please see the annotated GenBank file and the additional image file for the specific location of the minipromoter in this plasmid.
Proper citation: RRID:Addgene_49123 Copy
Species: Synthetic
Genetic Insert: ssAAV-Ple67-iCre-WPRE
Vector Backbone Description: Vector Backbone:AAV2; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30765420
Comments: Please see the annotated GenBank file and the additional image file for the specific location of the minipromoter in this plasmid.
Proper citation: RRID:Addgene_49122 Copy
Species: Synthetic
Genetic Insert: PercevalHR
Vector Backbone Description: Backbone Size:9222; Vector Backbone:FUGW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24096541
Proper citation: RRID:Addgene_49083 Copy
Species: Synthetic
Genetic Insert: PercevalHR
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2327; Vector Backbone:pRsetB; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24096541
Proper citation: RRID:Addgene_49081 Copy
Species: Synthetic
Genetic Insert: ssAAV-Ple155-iCre
Vector Backbone Description: Vector Backbone:AAV2; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33531684
Comments: Please see the annotated GenBank file and the additional image file for the specific location of the minipromoter in this plasmid.
Additional References:
1 Korecki, A. J. et al. Twenty-Seven Tamoxifen-Inducible iCre-Driver Mouse Strains for Eye and Brain, Including Seventeen Carrying a New Inducible-First Constitutive-Ready Allele. Genetics 211, 1155-1177 (2019).
2 de Leeuw, C. N. et al. rAAV-Compatible MiniPromoters for Restricted Expression in the Brain and Eye. Molecular Brain 9, 52 (2016).
3 Scalabrino, M. L. et al. Intravitreal delivery of a novel AAV vector targets ON bipolar cells and restores visual function in a mouse model of complete congenital stationary night blindness. Hum Mol Genet 24, 6229-6239, doi:10.1093/hmg/ddv341, ddv341 [pii] (2015).
4 de Leeuw, C. N. et al. Targeted CNS delivery using human MiniPromoters and demonstrated compatibility with adeno-associated viral vectors. Mol Ther Methods Clin Dev 1, 1-15 (2014).
Proper citation: RRID:Addgene_49116 Copy
Species: Synthetic
Genetic Insert: ssAAV-Ple253-iCre-WPRE
Vector Backbone Description: Vector Backbone:AAV2; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30062914
Comments: Please see the annotated GenBank file and the additional image file for the specific location of the minipromoter in this plasmid.
Proper citation: RRID:Addgene_49115 Copy
Species: Synthetic
Genetic Insert: ssAAV-CAGGS-iCre
Vector Backbone Description: Vector Backbone:AAV2; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30062914
Comments: Please see the annotated GenBank file and the additional image file for the specific location of the minipromoter in this plasmid.
Proper citation: RRID:Addgene_49119 Copy
Species: Synthetic
Genetic Insert: ssAAV-Ple253-iCre
Vector Backbone Description: Vector Backbone:AAV2; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30062914
Comments: Please see the annotated GenBank file and the additional image file for the specific location of the minipromoter in this plasmid.
Proper citation: RRID:Addgene_49114 Copy
Species: Synthetic
Genetic Insert: ssAAV-Ple261-iCre
Vector Backbone Description: Vector Backbone:AAV2; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31323276
Comments: Please see the annotated GenBank file and the additional image file for the specific location of the minipromoter in this plasmid.
Please see the following publication for more information:
rAAV-compatible MiniPromoters for restricted expression in the brain and eye.
de Leeuw et al. Mol Brain. 2016 May 10;9(1):52. doi: 10.1186/s13041-016-0232-4. PMID 27164903
Proper citation: RRID:Addgene_49113 Copy
Species: Synthetic
Genetic Insert: ssAAV-MCS-iCre
Vector Backbone Description: Vector Backbone:AAV2; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30062914
Comments: Please see the annotated GenBank file and the additional image file for the specific location of the minipromoter in this plasmid.
Proper citation: RRID:Addgene_49111 Copy
Species: Synthetic
Genetic Insert: ssAAV-Ple251-icre
Vector Backbone Description: Vector Backbone:AAV2; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30062914
Comments: See publication listed below for more details about the use of this plasmid and the expression pattern of iCre under the Ple251 promoter.
Please see the annotated GenBank file and the additional image file for the specific location of the minipromoter in this plasmid.
Proper citation: RRID:Addgene_49109 Copy
Species: Synthetic
Genetic Insert: mEGFP
Vector Backbone Description: Backbone Size:8754; Vector Backbone:Plasmid 12255: pWPT-GFP; Vector Types:Mammalian Expression, Lentiviral, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23798422
Comments: Vector allows for control of transgene expression at the level of translation. It is 1 vector in a set of 9 that spans a range of expression levels. Sequencing through any part of the IRES sequence is not advised. Expression of gene downstream of IRES remains constant, despite varying expression of gene upstream.
Proper citation: RRID:Addgene_49235 Copy
Species: Synthetic
Genetic Insert: mEGFP
Vector Backbone Description: Backbone Size:8754; Vector Backbone:Plasmid 12255: pWPT-GFP; Vector Types:Mammalian Expression, Lentiviral, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23798422
Comments: Vector allows for control of transgene expression at the level of translation. It is 1 vector in a set of 9 that spans a range of expression levels. Sequencing through any part of the IRES sequence is not advised. Expression of gene downstream of IRES remains constant, despite varying expression of gene upstream.
Proper citation: RRID:Addgene_49234 Copy
Species: Synthetic
Genetic Insert: mEGFP
Vector Backbone Description: Backbone Size:8754; Vector Backbone:Plasmid 12255: pWPT-GFP; Vector Types:Mammalian Expression, Lentiviral, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23798422
Comments: Vector allows for control of transgene expression at the level of translation. It is 1 vector in a set of 9 that spans a range of expression levels. Sequencing through any part of the IRES sequence is not advised. Expression of gene downstream of IRES remains constant, despite varying expression of gene upstream.
Proper citation: RRID:Addgene_49232 Copy
Species: Synthetic
Genetic Insert: mEGFP
Vector Backbone Description: Vector Backbone:pCru5; Vector Types:Mammalian Expression, Retroviral, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23798422
Comments: Vector allows for control of transgene expression at the level of translation. It is 1 vector in a set of 9 that spans a range of expression levels. Sequencing through any part of the IRES sequence is not advised. Expression of gene downstream of IRES remains constant, despite varying expression of gene upstream.
Proper citation: RRID:Addgene_49226 Copy
Species: Synthetic
Genetic Insert: mEGFP
Vector Backbone Description: Vector Backbone:pCru5; Vector Types:Mammalian Expression, Retroviral, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23798422
Comments: Vector allows for control of transgene expression at the level of translation. It is 1 vector in a set of 9 that spans a range of expression levels. Sequencing through any part of the IRES sequence is not advised. Expression of gene downstream of IRES remains constant, despite varying expression of gene upstream.
Proper citation: RRID:Addgene_49220 Copy
Species: Synthetic
Genetic Insert: mEGFP
Vector Backbone Description: Vector Backbone:pCru5; Vector Types:Mammalian Expression, Retroviral, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23798422
Comments: Vector allows for control of transgene expression at the level of translation. It is 1 vector in a set of 9 that spans a range of expression levels. Sequencing through any part of the IRES sequence is not advised. Expression of gene downstream of IRES remains constant, despite varying expression of gene upstream.
Proper citation: RRID:Addgene_49222 Copy
Species: Synthetic
Genetic Insert: FRT forward
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pBluescript; Vector Types:cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24116091
Proper citation: RRID:Addgene_49310 Copy
Species: Synthetic
Genetic Insert: luc2
Vector Backbone Description: Backbone Marker:Genscript; Backbone Size:2693; Vector Backbone:pUC57; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25177895
Proper citation: RRID:Addgene_49352 Copy
Species: Synthetic
Genetic Insert: luc2
Vector Backbone Description: Backbone Marker:Genscript; Backbone Size:2693; Vector Backbone:pUC57; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25177895
Proper citation: RRID:Addgene_49353 Copy
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