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Species: Danio rerio
Genetic Insert: CBS pair domain of zebrafish CNNM1
Vector Backbone Description: Vector Backbone:pGEX-6P-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_250561 Copy
Species: Danio rerio
Genetic Insert: zebrafish PRL3
Vector Backbone Description: Vector Backbone:pET-15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_250560 Copy
Species: Danio rerio
Genetic Insert: arrestin, beta 2a
Vector Backbone Description: Vector Backbone:pCS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_251767 Copy
Species: Danio rerio
Genetic Insert: bmp7
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCS2; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19377468
Proper citation: RRID:Addgene_201909 Copy
Species: Danio rerio
Genetic Insert: Targeted mRFP protein trap; secondary marker gamma crystallin GFP
Vector Backbone Description: Backbone Marker:K.Clark; Backbone Size:7200; Vector Backbone:pKTol2-SE; Vector Types:Targetable insertional mutagen and reporter system; Bacterial Resistance:Kanamycin
Comments: This plasmid is designed to have homology domains cloned into the 5' end and 3' end. Bfuai is used to clone in the 5' homology domain, while BspQi is used to clone in the 3' homology domain.
Proper citation: RRID:Addgene_187999 Copy
Species: Danio rerio
Genetic Insert: Targeted mRFP protein trap; secondary marker gamma crystallin GFP
Vector Backbone Description: Backbone Marker:K.Clark; Backbone Size:7200; Vector Backbone:pKTol2-SE; Vector Types:Targetable insertional mutagen and reporter system; Bacterial Resistance:Kanamycin
Comments: This plasmid is designed to have homology domains cloned into the 5' end and 3' end. Bfuai is used to clone in the 5' homology domain, while BspQi is used to clone in the 3' homology domain.
Proper citation: RRID:Addgene_187998 Copy
Species: Danio rerio
Genetic Insert: Targeted 2aGal4VP16 protein trap; secondary marker insulin GFP
Vector Backbone Description: Backbone Marker:K.Clark; Backbone Size:7200; Vector Backbone:pKTol2-SE; Vector Types:Targetable insertional mutagen and reporter system; Bacterial Resistance:Kanamycin
Comments: This plasmid is designed to have homology domains cloned into the 5' end and 3' end. Bfuai is used to clone in the 5' homology domain, while BspQi is used to clone in the 3' homology domain.
Proper citation: RRID:Addgene_188028 Copy
Species: Danio rerio
Genetic Insert: Targeted 2aGal4VP16 protein trap; secondary marker cmlc2 GFP
Vector Backbone Description: Backbone Marker:K.Clark; Backbone Size:7200; Vector Backbone:pKTol2-SE; Vector Types:Targetable insertional mutagen and reporter system; Bacterial Resistance:Kanamycin
Comments: This plasmid is designed to have homology domains cloned into the 5' end and 3' end. Bfuai is used to clone in the 5' homology domain, while BspQi is used to clone in the 3' homology domain.
Proper citation: RRID:Addgene_188022 Copy
Species: Danio rerio
Genetic Insert: Targeted mRFP protein trap; secondary marker cmlc2 BFP
Vector Backbone Description: Backbone Marker:K.Clark; Backbone Size:7200; Vector Backbone:pKTol2-SE; Vector Types:Targetable insertional mutagen and reporter system; Bacterial Resistance:Kanamycin
Comments: This plasmid is designed to have homology domains cloned into the 5' end and 3' end. Bfuai is used to clone in the 5' homology domain, while BspQi is used to clone in the 3' homology domain.
Proper citation: RRID:Addgene_188001 Copy
Species: Danio rerio
Genetic Insert: Targeted mRFP protein trap; secondary marker cmlc2 BFP
Vector Backbone Description: Backbone Marker:K.Clark; Backbone Size:7200; Vector Backbone:pKTol2-SE; Vector Types:Targetable insertional mutagen and reporter system; Bacterial Resistance:Kanamycin
Comments: This plasmid is designed to have homology domains cloned into the 5' end and 3' end. Bfuai is used to clone in the 5' homology domain, while BspQi is used to clone in the 3' homology domain.
Proper citation: RRID:Addgene_188002 Copy
Species: Danio rerio
Genetic Insert: Targeted mRFP protein trap; secondary marker cmlc2 GFP
Vector Backbone Description: Backbone Marker:K.Clark; Backbone Size:7200; Vector Backbone:pKTol2-SE; Vector Types:Targetable insertional mutagen and reporter system; Bacterial Resistance:Kanamycin
Comments: This plasmid is designed to have homology domains cloned into the 5' end and 3' end. Bfuai is used to clone in the 5' homology domain, while BspQi is used to clone in the 3' homology domain.
Proper citation: RRID:Addgene_188004 Copy
Species: Danio rerio
Genetic Insert: Targeted mRFP protein trap; secondary marker cmlc2 GFP
Vector Backbone Description: Backbone Marker:K.Clark; Backbone Size:7200; Vector Backbone:pKTol2-SE; Vector Types:Targetable insertional mutagen and reporter system; Bacterial Resistance:Kanamycin
Comments: This plasmid is designed to have homology domains cloned into the 5' end and 3' end. Bfuai is used to clone in the 5' homology domain, while BspQi is used to clone in the 3' homology domain.
Proper citation: RRID:Addgene_188006 Copy
Species: Danio rerio
Genetic Insert: Targeted mRFP protein trap; secondary marker insulin BFP
Vector Backbone Description: Backbone Marker:K.Clark; Backbone Size:7200; Vector Backbone:pKTol2-SE; Vector Types:Targetable insertional mutagen and reporter system; Bacterial Resistance:Kanamycin
Comments: This plasmid is designed to have homology domains cloned into the 5' end and 3' end. Bfuai is used to clone in the 5' homology domain, while BspQi is used to clone in the 3' homology domain.
Proper citation: RRID:Addgene_188009 Copy
Species: Danio rerio
Genetic Insert: Targeted 2aGal4VP16 protein trap; secondary marker gamma crystallin BFP
Vector Backbone Description: Backbone Marker:K.Clark; Backbone Size:7200; Vector Backbone:pKTol2-SE; Vector Types:Targetable insertional mutagen and reporter system; Bacterial Resistance:Kanamycin
Comments: This plasmid is designed to have homology domains cloned into the 5' end and 3' end. Bfuai is used to clone in the 5' homology domain, while BspQi is used to clone in the 3' homology domain.
Proper citation: RRID:Addgene_188013 Copy
Species: Danio rerio
Genetic Insert: Targeted 2aGal4VP16 protein trap; secondary marker gamma crystallin GFP
Vector Backbone Description: Backbone Marker:K.Clark; Backbone Size:7200; Vector Backbone:pKTol2-SE; Vector Types:Targetable insertional mutagen and reporter system; Bacterial Resistance:Kanamycin
Comments: This plasmid is designed to have homology domains cloned into the 5' end and 3' end. Bfuai is used to clone in the 5' homology domain, while BspQi is used to clone in the 3' homology domain.
Proper citation: RRID:Addgene_188016 Copy
Species: Danio rerio
Genetic Insert: Targeted 2aGal4VP16 protein trap; secondary marker gamma crystallin GFP
Vector Backbone Description: Backbone Marker:K.Clark; Backbone Size:7200; Vector Backbone:pKTol2-SE; Vector Types:Targetable insertional mutagen and reporter system; Bacterial Resistance:Kanamycin
Comments: This plasmid is designed to have homology domains cloned into the 5' end and 3' end. Bfuai is used to clone in the 5' homology domain, while BspQi is used to clone in the 3' homology domain.
Proper citation: RRID:Addgene_188018 Copy
Species: Danio rerio
Genetic Insert: Targeted mRFP protein trap; secondary marker insulin GFP
Vector Backbone Description: Backbone Marker:K.Clark; Backbone Size:7200; Vector Backbone:pKTol2-SE; Vector Types:Targetable insertional mutagen and reporter system; Bacterial Resistance:Kanamycin
Comments: This plasmid is designed to have homology domains cloned into the 5' end and 3' end. Bfuai is used to clone in the 5' homology domain, while BspQi is used to clone in the 3' homology domain.
Proper citation: RRID:Addgene_188011 Copy
Species: Danio rerio
Genetic Insert: nanos2
Vector Backbone Description: Backbone Marker:Christian Mosimann; Backbone Size:4974; Vector Backbone:pTol2Dest_pCY; Vector Types:Zebrafish expression; Bacterial Resistance:Ampicillin
Comments: Cloned by Maya Pahima.
nos2 control elements were cloned from zebrafish ovary gDNA.
Gateway recombination: pTol2-nos2L:d2egfp-nos2,3’utr, cryaa:Venus (pCY) was generated by combining p5E-nos2promL, pME-d2GFP (Collery and Link, 2011), and pCR4-nos2,3’utr (Chapter 6) into the pTol2-R4/R3_cryaa:Venus backbone vector (Mosimann et al., 2013).
Proper citation: RRID:Addgene_226801 Copy
Species: Danio rerio
Genetic Insert: Ace2N-mNeon
Vector Backbone Description: Vector Backbone:pDestTol pA2; Vector Types:Zebrafish expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:42109300
Comments: Please visit https://doi.org/10.1101/2024.12.12.628143 for bioRxiv preprint.
Proper citation: RRID:Addgene_253618 Copy
Species: Danio rerio
Genetic Insert: nanos2
Vector Backbone Description: Backbone Marker:Florence Marlow, Amanda Heim; Backbone Size:6946; Vector Backbone:pTol2Dest_pBH; Vector Types:Zebrafish expression; Bacterial Resistance:Ampicillin
Comments: Cloned by Maya Pahima.
pTol2-nos2L:mApple, cmlc2:dsred (pBH) was generated by combining p5E-nos2prom and pME-mApple (Addgene, 195960) (Kemmler et al., 2023) into the pTol2-R4/R2_cmlc2:dsred (pBH) backbone vector (Heim et al., 2014).
Proper citation: RRID:Addgene_226797 Copy
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