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Vector Backbone Description: Backbone Marker:PMID: 17878951; Backbone Size:5605; Vector Backbone:pDEST-Spe3-RfA; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17878951
Comments: Gateway destination vector for mRNA production to overexpress a protein of interest following microinjection into ascidian eggs. Likely to be useful for zebrafish, Xenopus, urchin and cnidarian (Clytia, Nematostella...) overexpression studies as well. Backbone was modified by insertion of C terminal Venus tag at EcoR V restriction site
Proper citation: RRID:Addgene_186372 Copy
Vector Backbone Description: Backbone Marker:PMID: 17878951; Backbone Size:5610; Vector Backbone:pDEST-Spe3-RfA; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17878951
Comments: Gateway destination vector for mRNA production to overexpress a protein of interest following microinjection into ascidian eggs. Likely to be useful for zebrafish, Xenopus, urchin and cnidarian (Clytia, Nematostella...) overexpression studies as well. Backbone was modified by insertion of N terminal Venus tag at Stu I restriction site
Proper citation: RRID:Addgene_186371 Copy
Vector Backbone Description: Backbone Size:5598; Vector Backbone:pDEST-Spe4-RfA; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: Gateway destination vector for mRNA production to overexpress a protein of interest following microinjection into ascidian eggs. Likely to be useful for zebrafish, Xenopus, urchin and cnidarian (Clytia, Nematostella...) overexpression studies as well. Backbone was modified by insertion of N terminal mClover3 tag at Bgl II/Stu I restriction site
Proper citation: RRID:Addgene_186379 Copy
Vector Backbone Description: Backbone Marker:PMID: 17878951; Backbone Size:7673; Vector Backbone:pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::RfA; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17878951
Comments: Gateway destination vector for electroporation experiments. Backbone was modified by insertion of C terminal ECFP tag at EcoR V restriction site
Proper citation: RRID:Addgene_186412 Copy
Vector Backbone Description: Backbone Marker:PMID: 17878951; Backbone Size:6994; Vector Backbone:pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::RfA; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17878951
Comments: Gateway destination vector for electroporation experiments. Backbone was modified by insertion of N terminal HA tag at Stu I restriction site
Proper citation: RRID:Addgene_186411 Copy
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4342; Vector Backbone:STARR-seq luciferase INF enhancer vector_ORI; Vector Types:Mammalian Expression, hSTARR-seq screening vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:29256496
Comments: This vector has a bGH poly A sequence upstream of the ORI and a RBG polyA and a bGH poly A sequence downstream of the ORI; for blocking transcripts arising from the ORI; Core promoter is SCP1 . Please see bioRxiv for preprint article. Posted 17 July 2017. DOI: 10.1101/164590
Proper citation: RRID:Addgene_99317 Copy
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4457; Vector Backbone:STARR-seq luciferase INF enhancer vector_ORI; Vector Types:Mammalian Expression, hSTARR-seq screening vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:29256496
Comments: This vector has a bcl 2 splice acceptor and 4x polyA sequences downstream of the ORI; for blocking transcripts arising from the ORI; Core promoter is SCP1 . Please see bioRxiv for preprint article. Posted 17 July 2017. DOI: 10.1101/164590
Proper citation: RRID:Addgene_99318 Copy
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:2807; Vector Backbone:hSTARR-seq_mCP vector; Vector Types:Mammalian Expression, hSTARR-seq screening vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:29256496
Comments: This screening vector can screen a DNA library which can be inserted via the AgeI and SalI restriction sides using subsequent In-Fusion reaction (Clontech) Please see bioRxiv for preprint article. Posted 17 July 2017. DOI: 10.1101/164590
Proper citation: RRID:Addgene_99291 Copy
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:2857; Vector Backbone:hSTARR-seq_TTF2 vector; Vector Types:Mammalian Expression, hSTARR-seq screening vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:29256496
Comments: This screening vector can screen a DNA library which can be inserted via the AgeI and SalI restriction sides using subsequent In-Fusion reaction (Clontech) . Please see bioRxiv for preprint article. Posted 17 July 2017. DOI: 10.1101/164590
Proper citation: RRID:Addgene_99293 Copy
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:2857; Vector Backbone:hSTARR-seq_OCT4 3UTR vector; Vector Types:Mammalian Expression, hSTARR-seq screening vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:29256496
Comments: This screening vector can screen a DNA library which can be inserted via the AgeI and SalI restriction sides using subsequent In-Fusion reaction (Clontech) . Please see bioRxiv for preprint article. Posted 17 July 2017. DOI: 10.1101/164590
Proper citation: RRID:Addgene_99295 Copy
Vector Backbone Description: Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:26850639
Proper citation: RRID:Addgene_99911 Copy
Vector Backbone Description: Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:26850639
Proper citation: RRID:Addgene_99912 Copy
Vector Backbone Description: Vector Backbone:pMOD_B0000; Vector Types:cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:36253612
Comments: MoClo compatible
Proper citation: RRID:Addgene_176874 Copy
Vector Backbone Description: Vector Backbone:pMOD_C'0000a; Vector Types:cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:36253612
Comments: MoClo compatible
Proper citation: RRID:Addgene_176875 Copy
Vector Backbone Description: Vector Backbone:pMOD_B0000; Vector Types:cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:36253612
Comments: MoClo compatible, allows transition from MoClo cloning system into the Cermak, et al., 2017 Golden Gate assembly system
Proper citation: RRID:Addgene_176883 Copy
Vector Backbone Description: Backbone Size:8409; Vector Backbone:pLX_TRC317 (https://portals.broadinstitute.org/gpp/public/vector/details?vector=pLX_TRC317); Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:35085500
Proper citation: RRID:Addgene_178534 Copy
Vector Backbone Description: Vector Backbone:pcg2.0; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:31308540
Proper citation: RRID:Addgene_180221 Copy
Vector Backbone Description: Backbone Marker:Thermofisher; Backbone Size:7603; Vector Backbone:pCDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:35182477
Proper citation: RRID:Addgene_182926 Copy
Species: Synthetic
Genetic Insert: sgRNA-scaffold only
Vector Backbone Description: Backbone Marker:Takara; Backbone Size:2125; Vector Backbone:pMD19; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:35969224
Proper citation: RRID:Addgene_190794 Copy
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4650; Vector Backbone:pAAV-MCS; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:28060929
Proper citation: RRID:Addgene_190226 Copy
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