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Genetic Insert: miniSOG
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2818; Vector Backbone:pCR8; Vector Types:Gateway TOPO Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:26861262
Proper citation: RRID:Addgene_74111 Copy
Species: Homo sapiens
Genetic Insert: Magneto2.0
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2817; Vector Backbone:pCR8; Vector Types:Gateway vector; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:26950006
Proper citation: RRID:Addgene_74334 Copy
Species: Homo sapiens
Genetic Insert: Magneto2.0
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCR8; Vector Types:Gateway vector; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:26950006
Proper citation: RRID:Addgene_74333 Copy
Species: Streptococcus pyogenes
Genetic Insert: hCas9
Vector Backbone Description: Backbone Marker:self-made; derived from pCambia1302 generated at the Cambia Institute; Backbone Size:6676; Vector Backbone:pDGB2omega2; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:26839579
Proper citation: RRID:Addgene_75400 Copy
Species: Escherichia coli
Genetic Insert: NptII
Vector Backbone Description: Backbone Marker:self-made; derived from pCambia1302 generated at the Cambia Institute; Backbone Size:6698; Vector Backbone:pDGB3omega1R; Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:26839579
Proper citation: RRID:Addgene_75471 Copy
Species: S. pyogenes
Genetic Insert: Cas9
Vector Backbone Description: Vector Backbone:pSC101ts; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:26141150
Comments: This plasmid is used for CRISPR mediated genome editing of E. coli
Proper citation: RRID:Addgene_71541 Copy
Species: Synthetic
Genetic Insert: mRuby2_PUFa
Vector Backbone Description: Vector Backbone:pCR8/GW/TOPO; Vector Types:Other, Gateway Donor; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:26768771
Comments: Please visit http://casil.io for more information, updates, and protocols. For more information on Cheng Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/albert-cheng/
Proper citation: RRID:Addgene_71902 Copy
Genetic Insert: 4xNLS-PUFw-2xNLS
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCR8/GW/TOPO; Vector Types:; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:26768771
Comments: Please visit http://casil.io for more information, updates, and protocols. For more information on Cheng Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/albert-cheng/
Proper citation: RRID:Addgene_71905 Copy
Species: Synthetic
Genetic Insert: 4xNLS-PUFc-2xNLS
Vector Backbone Description: Vector Backbone:pCR8/GW/TOPO; Vector Types:Other, Gateway Donor; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:26768771
Comments: Please visit http://casil.io for more information, updates, and protocols. For more information on Cheng Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/albert-cheng/
Proper citation: RRID:Addgene_71906 Copy
Species: S. pyogenes
Genetic Insert: dCas9 from S. pyogenes
Vector Backbone Description: Backbone Marker:Takara; Vector Backbone:pMD19T simple; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:26689101
Proper citation: RRID:Addgene_73223 Copy
Species: S. pyogenes
Genetic Insert: dCas9 from S. pyogenes
Vector Backbone Description: Backbone Marker:Takara; Vector Backbone:pMD19T simple; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:26689101
Proper citation: RRID:Addgene_73220 Copy
Species: Synthetic
Genetic Insert: BFPKRAB_PUFa
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCR8/GW/TOPO; Vector Types:; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:26768771
Comments: Please visit http://casil.io for more information, updates and protocols
Proper citation: RRID:Addgene_73692 Copy
Species: Synthetic
Genetic Insert: ratiometric pHluorin2
Vector Backbone Description: Vector Backbone:PCR8; Vector Types:middle entry clone; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24973752
Comments: Ratiometric pHluorin2 was originally published in the following paper:
Mahon, M. (2011) pHluorin2: an enhanced, ratiometric, pH-sensitive green florescent protein. Advances in Bioscience and Biotechnology, 2, 132-137. doi: 10.4236/abb.2011.23021.
Proper citation: RRID:Addgene_73794 Copy
Vector Backbone Description: Backbone Marker:Tsuyoshi Nakagawa, Shimane University; Backbone Size:9135; Vector Backbone:pGWB400; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:26406247
Proper citation: RRID:Addgene_68062 Copy
Species: Other
Genetic Insert: Solanum lycopersicum intergenic region
Vector Backbone Description: Backbone Marker:self-made; derived from pGreenII generated at the JIC; Vector Backbone:pDGB1_omega2; Vector Types:Synthetic Biology, Unspecified; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:23669743
Comments: insert can be released with BsaI
Proper citation: RRID:Addgene_68243 Copy
Species: Other
Genetic Insert: Solanum lycopersicum intergenic region
Vector Backbone Description: Backbone Marker:self-made; derived from pGreenII generated at the JIC; Vector Backbone:pDGB1_omega1; Vector Types:Synthetic Biology, Unspecified; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:23669743
Comments: insert can be released with BsaI
Proper citation: RRID:Addgene_68242 Copy
Species: Other
Genetic Insert: empty vector
Vector Backbone Description: Backbone Marker:self-made; derived from pCambia1302 generated at the Cambia Institute; Vector Backbone:pDGB3_omega2R; Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:23669743
Proper citation: RRID:Addgene_68241 Copy
Species: Other
Genetic Insert: empty vector
Vector Backbone Description: Backbone Marker:self-made; derived from pCambia1302 generated at the Cambia Institute; Vector Backbone:pDGB3_omega1R; Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:23669743
Proper citation: RRID:Addgene_68240 Copy
Species: E. coli
Genetic Insert: Coding sequence, hygromycin phophotransferase II (Escherichia coli)
Vector Backbone Description: Vector Backbone:pICH41308 AddGene #47998; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:26616834
Proper citation: RRID:Addgene_68259 Copy
Species: Zea mays
Genetic Insert: Promoter and 5UTR Ubiquitin (Zea mays)
Vector Backbone Description: Vector Backbone:pICH41295 AddGene #47997; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:26616834
Proper citation: RRID:Addgene_68257 Copy
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