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Species: Caenorhabditis elegans
Genetic Insert: phsp16.41::GFP-F64LS65T::(Gly)5Ala
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2643; Vector Backbone:pDONRP4P1R; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21637852
Proper citation: RRID:Addgene_21544 Copy
Species: Caenorhabditis elegans
Genetic Insert: unc-119:mCherry
Vector Backbone Description: Backbone Marker:Epicentre; Backbone Size:300; Vector Backbone:R6K origin PCR fragment; Vector Types:Worm Expression, Adds unc-119 marker to Amp resistant plasmids; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19520111
Comments: This plasmid has small deletions within the KanR gene. We generated these fragments by PCR so it is definitely possible that these were generated during amplification, these changes must not impair function as the construct is Kan resistant both as a plasmid and following recombination.
The other mismatches in 21596 are in a polylinker between the KanR gene and the unc-119 3’ UTR and should not cause any problems.
Proper citation: RRID:Addgene_21596 Copy
Species: Caenorhabditis elegans
Genetic Insert: pmex-5 (w/o ATG)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2643; Vector Backbone:pDONR P4-P1R; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21637852
Proper citation: RRID:Addgene_21512 Copy
Species: Caenorhabditis elegans
Genetic Insert: (Gly)5Ala::GFP-F64LS65T::tbb-2 3'UTR
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2639; Vector Backbone:pDONR P2R-P3; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21637852
Proper citation: RRID:Addgene_21510 Copy
Species: Caenorhabditis elegans
Genetic Insert: pmex-5::citrine (w introns)::(Gly)5Ala
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2643; Vector Backbone:pDONRP4P1R; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21637852
Proper citation: RRID:Addgene_21508 Copy
Species: Caenorhabditis elegans
Genetic Insert: pmex-5::GFP-F64LS65T::(Gly)5Ala
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2643; Vector Backbone:pDONRP4P1R; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21637852
Proper citation: RRID:Addgene_21507 Copy
Species: Caenorhabditis elegans
Genetic Insert: pmex-5::GFP (w introns)::(Gly)5Ala
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2643; Vector Backbone:pDONRP4P1R; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21637852
Proper citation: RRID:Addgene_21506 Copy
Species: Caenorhabditis elegans
Genetic Insert: pie-1>mCherry::PH domain (PLC-delta)
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:5712; Vector Backbone:pBS Sk-; Vector Types:bacterial propagation; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19098902
Proper citation: RRID:Addgene_21743 Copy
Species: Caenorhabditis elegans
Genetic Insert: abu-1 promoter
Vector Backbone Description: Backbone Marker:Available at Addgene (#1494); Backbone Size:4487; Vector Backbone:pPD95_75; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12186849
Comments: Addgene sequencing results show several mismatches with author's sequence.
abu-1::gfp C. elegans reporter gene. A 1.3-kb fragment of C. elegans genomic DNA immediately 5' of the predicted initiation ATG codon of abu-1 (AC3.3) was amplified by PCR using the oligonucleotides AC3.1S (5'-GGCATTGTGGCACGCATTGAACTG-3') and AC3Bam.2AS (5'-GATAGGATCCATTGTTAATATGCTTGAAGAGCTGC-3') and ligated in frame with the GFP coding region in the plasmid of pPD95.75.
Proper citation: RRID:Addgene_21824 Copy
Species: Caenorhabditis elegans
Genetic Insert: abu-1 promoter and coding region
Vector Backbone Description: Backbone Marker:Available at Addgene (#1494); Backbone Size:4487; Vector Backbone:pPD95_75; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12186849
Comments: Addgene sequencing results show there is a S408P substitution in amino acid sequence in the coding region.
abu-1::abu-1::gfp C. elegans reporter gene. The promoter and coding region of abu-1 was ligated in frame with GFP to produce abu1::abu-1::gfp
Proper citation: RRID:Addgene_21825 Copy
Species: Caenorhabditis elegans
Genetic Insert: eft-3 promoter
Vector Backbone Description: Backbone Size:2981; Vector Backbone:pCFJ1662; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37384903
Proper citation: RRID:Addgene_202331 Copy
Species: Caenorhabditis elegans
Genetic Insert: act-3p(long) promoter
Vector Backbone Description: Backbone Size:2647; Vector Backbone:pBR332; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37384903
Proper citation: RRID:Addgene_202330 Copy
Species: Caenorhabditis elegans
Genetic Insert: GLB-31
Vector Backbone Description: Vector Backbone:pET303/CT-His; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_224141 Copy
Species: Caenorhabditis elegans
Genetic Insert: GLB-21
Vector Backbone Description: Vector Backbone:pET303/CT-His; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_224140 Copy
Species: Caenorhabditis elegans
Genetic Insert: GLB-1
Vector Backbone Description: Vector Backbone:pET303/CT-His; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_224138 Copy
Species: Caenorhabditis elegans
Genetic Insert: GLB-10
Vector Backbone Description: Vector Backbone:pET303/CT-His; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_224139 Copy
Species: Caenorhabditis elegans
Genetic Insert: GtAR2
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:7216; Vector Backbone:pBSK; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38598630
Comments: Please visit for https://www.biorxiv.org/content/10.1101/2021.09.21.461278v4 bioRxiv preprint.
Proper citation: RRID:Addgene_200780 Copy
Species: Caenorhabditis elegans
Genetic Insert: GtACR2
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:8806; Vector Backbone:pBSK; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38598630
Comments: Please visit for https://www.biorxiv.org/content/10.1101/2021.09.21.461278v4 bioRxiv preprint.
Proper citation: RRID:Addgene_200781 Copy
Species: Caenorhabditis elegans
Genetic Insert: twk-40
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:9792; Vector Backbone:pBSK; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38598630
Comments: Please visit for https://www.biorxiv.org/content/10.1101/2021.09.21.461278v4 bioRxiv preprint.
Proper citation: RRID:Addgene_200160 Copy
Species: Caenorhabditis elegans
Genetic Insert: zif-1
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:10341; Vector Backbone:pBSK; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38598630
Comments: Please visit for https://www.biorxiv.org/content/10.1101/2021.09.21.461278v4 bioRxiv preprint.
Proper citation: RRID:Addgene_200043 Copy
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