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Species: Saccharomyces cerevisiae
Genetic Insert: YPT1
Vector Backbone Description: Backbone Marker:pRS416; Backbone Size:7497; Vector Backbone:pAG416GPD; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18162536
Proper citation: RRID:Addgene_18848 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: CHC1
Vector Backbone Description: Backbone Marker:pRS416; Backbone Size:7497; Vector Backbone:pAG416GPD; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18162536
Proper citation: RRID:Addgene_18850 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SEC7
Vector Backbone Description: Backbone Marker:pRS416; Backbone Size:7497; Vector Backbone:pAG416GPD; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18162536
Proper citation: RRID:Addgene_18851 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: truncated 3-hydroxy-3-methyl-glutaryl-coenzyme A Reductase
Vector Backbone Description: Backbone Marker:Guzman et al.; Backbone Size:0; Vector Backbone:pBAD18; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17239639
Proper citation: RRID:Addgene_19041 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Protein DOM34
Vector Backbone Description: Backbone Size:5443; Vector Backbone:pET21a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17889667
Proper citation: RRID:Addgene_19111 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Protein DOM34
Vector Backbone Description: Backbone Size:5443; Vector Backbone:pET21a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17889667
Proper citation: RRID:Addgene_19112 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Protein DOM34
Vector Backbone Description: Backbone Size:5369; Vector Backbone:pET28b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17889667
Proper citation: RRID:Addgene_19102 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Protein DOM34
Vector Backbone Description: Backbone Size:5443; Vector Backbone:pET21a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17889667
Proper citation: RRID:Addgene_19109 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SSA1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5137; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18686016
Comments: Part of the HSP70 gene family
Disclaimer:
For cloning the various HSP genes, the Stratagene reference RNA for cDNA synthesis was used and genes were amplified from this mixture that originates from 10 different cell lines from different somatic origin and ultimately coming from 10 different individuals. This strategy was used to maximize the chance for the presence of individual HSP transcripts. Users must be aware that this mixture thus represents 20 complete haploid genomes, meaning that for each individual gene there might be polymorphisms that could potentially have functional consequences. The library was sequence verified only for the presence of the correct gene.
Proper citation: RRID:Addgene_19516 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: FLP
Vector Backbone Description: Backbone Size:0; Vector Backbone:pBluescript SK+; Vector Types:carries FLP recombinase cDNA; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18723890
Proper citation: RRID:Addgene_19615 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: NAB3
Vector Backbone Description: Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22564898
Proper citation: RRID:Addgene_110535 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: NAB3
Vector Backbone Description: Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22564898
Proper citation: RRID:Addgene_110533 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: NAB3
Vector Backbone Description: Vector Backbone:pRS315; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26954508
Proper citation: RRID:Addgene_110538 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: FSF1
Vector Backbone Description: Backbone Marker:Cell Biolabs, Inc.; Backbone Size:5600; Vector Backbone:pMXs-IRES-Blasticidin; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30442778
Proper citation: RRID:Addgene_110641 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HSP82
Vector Backbone Description: Backbone Marker:ATCC (#77189); Backbone Size:4453; Vector Backbone:pRS303; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9990037
Proper citation: RRID:Addgene_1110 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HSP82
Vector Backbone Description: Backbone Marker:ATCC (#77189); Backbone Size:4453; Vector Backbone:pRS303; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9990037
Proper citation: RRID:Addgene_1111 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HSP82
Vector Backbone Description: Backbone Marker:ATCC (#77189); Backbone Size:4453; Vector Backbone:pRS303; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9990037
Proper citation: RRID:Addgene_1112 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SUB2
Vector Backbone Description: Backbone Size:6018; Vector Backbone:pRS315; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11156604
Proper citation: RRID:Addgene_111302 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PRP5
Vector Backbone Description: Backbone Size:7950; Vector Backbone:yCP50; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8722763
Comments: Full plasmid sequence contains several ambiguous bases in the backbone- Unsure if these regions will affect function.
Proper citation: RRID:Addgene_111303 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SNU114
Vector Backbone Description: Backbone Size:4887; Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15911574
Proper citation: RRID:Addgene_111308 Copy
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